miR-491-5p suppresses cell growth and invasion by targeting Notch3 in nasopharyngeal carcinoma

  • Authors:
    • Qi Zhang
    • Qiang Li
    • Tao Xu
    • Hui Jiang
    • Lin-Gen Xu
  • View Affiliations

  • Published online on: March 29, 2016     https://doi.org/10.3892/or.2016.4713
  • Pages: 3541-3547
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRNAs) have critical roles in the progression of nasopharyngeal carcinoma (NPC), a highly invasive and metastatic cancer that is widely prevalent in Southern China. miR-491-5p has been implicated in multiple types of cancer; however, its biological role and underlying mechanism in NPC have not been fully explored. In the present study, we found that miR-491-5p was downregulated in NPC tissues and cell lines compared with the corresponding normal counterparts. Overexpression of miR-491-5p significantly inhibited cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. Using miRNA target prediction algorithms and reporter assays, we showed that miR-491-5p suppressed Notch3 expression both at the mRNA and protein level through directly targeting the 3' untranslated region (3'-UTR) of Notch3 mRNA. Overexpression of Notch3 significantly reversed the tumor-suppressive effects of miR‑491-5p. Taken together, the present study reveals a mechanistic link between miR-491-5p and Notch3 in the pathogenesis of NPC and that miR-491-5p has potential as a therapeutic target for NPC.
View Figures
View References

Related Articles

Journal Cover

June-2016
Volume 35 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Li Q, Xu T, Jiang H and Xu L: miR-491-5p suppresses cell growth and invasion by targeting Notch3 in nasopharyngeal carcinoma. Oncol Rep 35: 3541-3547, 2016
APA
Zhang, Q., Li, Q., Xu, T., Jiang, H., & Xu, L. (2016). miR-491-5p suppresses cell growth and invasion by targeting Notch3 in nasopharyngeal carcinoma. Oncology Reports, 35, 3541-3547. https://doi.org/10.3892/or.2016.4713
MLA
Zhang, Q., Li, Q., Xu, T., Jiang, H., Xu, L."miR-491-5p suppresses cell growth and invasion by targeting Notch3 in nasopharyngeal carcinoma". Oncology Reports 35.6 (2016): 3541-3547.
Chicago
Zhang, Q., Li, Q., Xu, T., Jiang, H., Xu, L."miR-491-5p suppresses cell growth and invasion by targeting Notch3 in nasopharyngeal carcinoma". Oncology Reports 35, no. 6 (2016): 3541-3547. https://doi.org/10.3892/or.2016.4713