MicroRNA-130a promotes the metastasis and epithelial-mesenchymal transition of osteosarcoma by targeting PTEN

Chen,Jiansong; Yan,Dingding; Wu,Weiliang; Zhu,Jian; Ye,Wensong; Shu,Qiang

doi:10.3892/or.2016.4719

MicroRNA-130a promotes the metastasis and epithelial-mesenchymal transition of osteosarcoma by targeting PTEN

Authors:
- Jiansong Chen
- Dingding Yan
- Weiliang Wu
- Jian Zhu
- Wensong Ye
- Qiang Shu
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Affiliations: Department of Orthopaedics, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China, Department of Gynecologic Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, P.R. China, Department of Tumor Surgery, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China
Published online on: March 31, 2016 https://doi.org/10.3892/or.2016.4719
Pages: 3285-3292

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Abstract

MicroRNAs, which serve as post-transcriptional modulators of numerous genes, have been found to be important regulators during the pathogenesis of osteosarcoma. This study demonstrates for the first time that microRNA-130a (miR-130a) is significantly upregulated in osteosarcoma, and associated with the metastasis of osteosarcoma. Elevated level of miR-130a was closely correlated with poor clinical features and prognosis of osteosarcoma patients. In vitro assays revealed that miR-130a could potentiate the migration, invasion and the epithelial-mesenchymal transtion (EMT) of osteosarcoma cells. Moreover, phosphatase and tensin homolog (PTEN) was confirmed as not only a direct downstream target but also a functional mediator of miR-130a. MiR-130a exerted promoting effects on metastatic behavior and EMT of osteosarcoma cells through suppressing PTEN expression. Based on these findings, we conclude that miR-130a is a promising prognostic biomarker for osteosarcoma patients, and targeting miR-130a may be a potential treatment option for osteosarcoma patients with metastasis.

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