Transcriptional network in ovarian cancer cell line SKOV3 treated with Pinellia pedatisecta Schott extract

Zhou,Li; Xu,Teng; Zhang,Ying; Zhu,Mei; Zhu,Wen; Wang,Ziqiang; Gu,Hangzhi; Wang,Hanchu; Li,Peizhen; Ying,Jun; Yang,Lei; Ren,Ping; Li,Jinsong; Xu,Zuyuan; Ni,Liyan; Bao,Qiyu; Chen,Jindong

doi:10.3892/or.2016.4779

Transcriptional network in ovarian cancer cell line SKOV3 treated with Pinellia pedatisecta Schott extract

Authors:
- Li Zhou
- Teng Xu
- Ying Zhang
- Mei Zhu
- Wen Zhu
- Ziqiang Wang
- Hangzhi Gu
- Hanchu Wang
- Peizhen Li
- Jun Ying
- Lei Yang
- Ping Ren
- Jinsong Li
- Zuyuan Xu
- Liyan Ni
- Qiyu Bao
- Jindong Chen
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Affiliations: The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, University of Rochester Medical Center, Rochester, NY 14642, USA
Published online on: April 28, 2016 https://doi.org/10.3892/or.2016.4779
Pages: 462-470

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Abstract

Ovarian cancer is the most lethal disease among the malignant tumors of female reproductive organs. Few successful therapeutic options exist for patients with ovarian cancer. The common therapeutic methods are surgical operation, chemotherapy, radiotherapy, and combination of these treatments. In recent years, studies have indicated that Pinellia pedatisecta Schott (PPS), a traditional Chinese medicine, could inhibit tumor growth. In this study, we demonstrated that PPS extract could induce apoptosis in SKOV3 cells in a dose- and time-dependent manner. We further conducted transcriptome sequencing on PPS extract-treated SKOV3 cells along with controls, and identified 1,754 transcripts whose expression differs at least 3-fold over the controls. These differentially expressed transcripts include the apoptosis-related genes such as the caspase family members, and were significantly enriched in steroid biosynthesis in the KEGG pathway database compared with the transcriptome background. Most of the differentially expressed transcripts from this pathway were upregulated in PPS extract-treated cell line, indicating that PPS extract-induced apoptosis was accompanied by increased steroid biosynthesis (e.g. zymosterol). These results suggest that PPS extract could be a new cytostatic therapeutic agent for ovarian cancer.

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