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Article

MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer

  • Authors:
    • Qin Liu
    • Wei Wang
    • Xiongfa Yang
    • Dongxiao Zhao
    • Fangqiong Li
    • Hai Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, P.R. China, Key Laboratory of Organosilicon Chemistry and Material Technology of the Chinese Education Ministry, Hangzhou Normal University, Hangzhou, Zhejiang 310012, P.R. China
  • Pages: 189-196
    |
    Published online on: May 6, 2016
       https://doi.org/10.3892/or.2016.4788
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Abstract

MicroRNAs (miRNAs) function as genetic modulators that regulate gene expression and are involved in a wide range of biological roles, including tumor cell migration and invasion. In the present study, we demonstrated that the migration and invasion activity in MDA-MB-231 breast cancer cells could be directly influenced by altering miR-146a expression. The expression of RhoA and miR-146a in the breast cancer cells showed an inverse correlation. Upregulation of miR-146a in the MDA-MB‑231 breast cancer cells by transfection of miR-146a mimics resulted in decreased RhoA protein levels. Conversely, downregulation of miR-146a by transfection of miR-146a inhibitor resulted in increased RhoA protein levels. To confirm the fact that RhoA is a potential target of miR-146a, luciferase reporter containing the RhoA 3' untranslated region (3'UTR) was constructed. The results demonstrated that the luciferase reporter activity was reduced after overexpression of miR-146a. Moreover, the luciferase reporter which was constructed with the RhoA 3'UTR mutant did not show significantly altered luciferase reporter activity. Furthermore, after treatment with the RhoA inhibitor exoenzyme C3 transferase protein, the migratory capacity of the MDA-MB-231 cells was not significantly altered even though the amount of miR-146a was changed. Our results indicate that miR-146a functions as a tumor suppressor in breast cancer cells. Downregulation of the expression of miR-146a increased the migration of MDA-MB-231 cells, due to the upregulation of RhoA expression.
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1 

Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T and Thun MJ: Cancer statistics, 2008. CA Cancer J Clin. 58:71–96. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Zegers MM and Friedl P: Rho GTPases in collective cell migration. Small GTPases. 5:e289972014. View Article : Google Scholar : PubMed/NCBI

3 

Gonzalez-Billault C, Muñoz-Llancao P, Henriquez DR, Wojnacki J, Conde C and Caceres A: The role of small GTPases in neuronal morphogenesis and polarity. Cytoskeleton Hoboken. 69:464–485. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Chircop M: Rho GTPases as regulators of mitosis and cytokinesis in mammalian cells. Small GTPases. 5(5)2014. View Article : Google Scholar : PubMed/NCBI

5 

Jordan SN and Canman JC: Rho GTPases in animal cell cytokinesis: An occupation by the one percent. Cytoskeleton Hoboken. 69:919–930. 2012. View Article : Google Scholar : PubMed/NCBI

6 

O'Connor K and Chen M: Dynamic functions of RhoA in tumor cell migration and invasion. Small GTPases. 4:141–147. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Orgaz JL, Herraiz C and Sanz-Moreno V: Rho GTPases modulate malignant transformation of tumor cells. Small GTPases. 5:e290192014. View Article : Google Scholar : PubMed/NCBI

8 

Ariake K, Ohtsuka H, Motoi F, Douchi D, Oikawa M, Rikiyama T, Fukase K, Katayose Y, Egawa S and Unno M: GCF2/LRRFIP1 promotes colorectal cancer metastasis and liver invasion through integrin-dependent RhoA activation. Cancer Lett. 325:99–107. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Cai K, Mulatz K, Ard R, Nguyen T and Gee SH: increased diacylglycerol kinase ζ expression in human metastatic colon cancer cells augments Rho GTPase activity and contributes to enhanced invasion. BMC Cancer. 14:2082014. View Article : Google Scholar

10 

Alho I, Costa L, Bicho M and Coelho C: Low molecular weight protein tyrosine phosphatase isoforms regulate breast cancer cells migration through a RhoA dependent mechanism. PLoS One. 8:e763072013. View Article : Google Scholar : PubMed/NCBI

11 

Dulong C, Fang YJ, Gest C, Zhou MH, Patte-Mensah C, Mensah-Nyagan AG, Vannier JP, Lu H, Soria C, Cazin L, et al: The small GTPase RhoA regulates the expression and function of the sodium channel Nav1.5 in breast cancer cells. Int J Oncol. 44:539–547. 2014.

12 

Hu T, Guo H, Wang W, Yu S, Han L, Jiang L, Ma J, Yang C, Guo Q and Nan K: Loss of p57 expression and RhoA overexpression are associated with poor survival of patients with hepatocellular carcinoma. Oncol Rep. 30:1707–1714. 2013.PubMed/NCBI

13 

Wang SC, Lin XL, Li J, Zhang TT, Wang HY, Shi JW, Yang S, Zhao WT, Xie RY, Wei F, et al: MicroRNA-122 triggers mesenchymal-epithelial transition and suppresses hepatocellular carcinoma cell motility and invasion by targeting RhoA. PLoS One. 9:e1013302014. View Article : Google Scholar : PubMed/NCBI

14 

Duan JT, Wang XM, Zhang SQ and Zhao GJ: Effect of RhoA gene silencing on proliferation and migration of gastric MGC-803 cells. Int J Clin Exp Med. 8:14410–14415. 2015.PubMed/NCBI

15 

Zhou J, Hayakawa Y, Wang TC and Bass AJ: RhoA mutations identified in diffuse gastric cancer. Cancer Cell. 26:9–11. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Guan R, Xu X, Chen M, Hu H, Ge H, Wen S, Zhou S and Pi R: Advances in the studies of roles of Rho/Rho-kinase in diseases and the development of its inhibitors. Eur J Med Chem. 70:613–622. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Yu OM and Brown JH: G Protein-coupled receptor and RhoA-stimulated transcriptional responses: Links to inflammation, differentiation, and cell proliferation. Mol Pharmacol. 88:171–180. 2015. View Article : Google Scholar : PubMed/NCBI

18 

De Ruyck K, Duprez F, Ferdinande L, Mbah C, Rios-Velazquez E, Hoebers F, Praet M, Deron P, Bonte K, Speel EJ, et al: A let-7 microRNA polymorphism in the KRAS3′-UTR is prognostic in oropharyngeal cancer. Cancer Epidemiol. 38:591–598. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Kong W, Yang H, He L, Zhao JJ, Coppola D, Dalton WS and Cheng JQ: MicroRNA-155 is regulated by the transforming growth factor beta/Smad pathway and contributes to epithelial cell plasticity by targeting RhoA. Mol Cell Biol. 28:6773–6784. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Liu M, Lang N, Chen X, Tang Q, Liu S, Huang J, Zheng Y and Bi F: miR-185 targets RhoA and Cdc42 expression and inhibits the proliferation potential of human colorectal cells. Cancer Lett. 301:151–160. 2011. View Article : Google Scholar

21 

Liu C and Tang DG: MicroRNA regulation of cancer stem cells. Cancer Res. 71:5950–5954. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Nair VS, Maeda LS and Ioannidis JP: Clinical outcome prediction by microRNAs in human cancer: A systematic review. J Natl Cancer Inst. 104:528–540. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Tang J, Ahmad A and Sarkar FH: The role of microRNAs in breast cancer migration, invasion and metastasis. Int J Mol Sci. 13:13414–13437. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Bhaumik D, Scott GK, Schokrpur S, Patil CK, Campisi J and Benz CC: Expression of microRNA-146 suppresses NF-kappaB activity with reduction of metastatic potential in breast cancer cells. Oncogene. 27:5643–5647. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Chen G, Umelo IA, Lv S, Teugels E, Fostier K, Kronenberger P, Dewaele A, Sadones J, Geers C and De Grève J: miR-146a inhibits cell growth, cell migration and induces apoptosis in non-small cell lung cancer cells. PLoS One. 8:e603172013. View Article : Google Scholar : PubMed/NCBI

26 

Sun Q, Zhao X, Liu X, Wang Y, Huang J, Jiang B, Chen Q and Yu J: miR-146a functions as a tumor suppressor in prostate cancer by targeting Rac1. Prostate. 74:1613–1621. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Zheng T, Chou J, Zhang F, Liu Y, Ni H, Li X, Zheng L, Tang T, Jin L and Xi T: CXCR4 3′UTR functions as a ceRNA in promoting metastasis, proliferation and survival of MCF-7 cells by regulating miR-146a activity. Eur J Cell Biol. 94:458–469. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Citi S, Spadaro D, Schneider Y, Stutz J and Pulimeno P: Regulation of small GTPases at epithelial cell-cell junctions. Mol Membr Biol. 28:427–444. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Wojnacki J, Quassollo G, Marzolo MP and Cáceres A: Rho GTPases at the crossroad of signaling networks in mammals: Impact of Rho-GTPases on microtubule organization and dynamics. Small GTPases. 5:e284302014. View Article : Google Scholar : PubMed/NCBI

30 

Lovat F, Valeri N and Croce CM: MicroRNAs in the pathogenesis of cancer. Semin Oncol. 38:724–733. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Hou Z, Xie L, Yu L, Qian X and Liu B: MicroRNA-146a is downregulated in gastric cancer and regulates cell proliferation and apoptosis. Med Oncol. 29:886–892. 2012. View Article : Google Scholar

32 

Li H, Xie S, Liu M, Chen Z, Liu X, Wang L, Li D and Zhou Y: The clinical significance of downregulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p in gastric cancer tumorigenesis. Int J Oncol. 45:197–208. 2014.PubMed/NCBI

33 

Li Y, Vandenboom TG II, Wang Z, Kong D, Ali S, Philip PA and Sarkar FH: miR-146a suppresses invasion of pancreatic cancer cells. Cancer Res. 70:1486–1495. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Matsuoka T and Yashiro M: Rho/ROCK signaling in motility and metastasis of gastric cancer. World J Gastroenterol. 20:13756–13766. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Baranwal S and Alahari SK: Rho GTPase effector functions in tumor cell invasion and metastasis. Curr Drug Targets. 12:1194–1201. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Duluc L and Wojciak-Stothard B: Rho GTPases in the regulation of pulmonary vascular barrier function. Cell Tissue Res. 355:675–685. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Ellenbroek SI and Collard JG: Rho GTPases: Functions and association with cancer. Clin Exp Metastasis. 24:657–672. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Street CA and Bryan BA: Rho kinase proteins - pleiotropic modulators of cell survival and apoptosis. Anticancer Res. 31:3645–3657. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Q, Wang W, Yang X, Zhao D, Li F and Wang H: MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer. Oncol Rep 36: 189-196, 2016.
APA
Liu, Q., Wang, W., Yang, X., Zhao, D., Li, F., & Wang, H. (2016). MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer. Oncology Reports, 36, 189-196. https://doi.org/10.3892/or.2016.4788
MLA
Liu, Q., Wang, W., Yang, X., Zhao, D., Li, F., Wang, H."MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer". Oncology Reports 36.1 (2016): 189-196.
Chicago
Liu, Q., Wang, W., Yang, X., Zhao, D., Li, F., Wang, H."MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer". Oncology Reports 36, no. 1 (2016): 189-196. https://doi.org/10.3892/or.2016.4788
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Q, Wang W, Yang X, Zhao D, Li F and Wang H: MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer. Oncol Rep 36: 189-196, 2016.
APA
Liu, Q., Wang, W., Yang, X., Zhao, D., Li, F., & Wang, H. (2016). MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer. Oncology Reports, 36, 189-196. https://doi.org/10.3892/or.2016.4788
MLA
Liu, Q., Wang, W., Yang, X., Zhao, D., Li, F., Wang, H."MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer". Oncology Reports 36.1 (2016): 189-196.
Chicago
Liu, Q., Wang, W., Yang, X., Zhao, D., Li, F., Wang, H."MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer". Oncology Reports 36, no. 1 (2016): 189-196. https://doi.org/10.3892/or.2016.4788
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