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Article

Nuclear EpICD expression and its role in hepatocellular carcinoma

  • Authors:
    • Shin Young Park
    • Jun Sang Bae
    • Eun Jung Cha
    • Hyun Hee Chu
    • Jang Sihn Sohn
    • Woo Sung Moon
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Konyang University College of Medicine, Daejeon 35365, Republic of Korea, Department of Pathology, Chonbuk National University, Medical School Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital and Research Institute for Endocrine Sciences, Jeonju 561-756, Republic of Korea
  • Pages: 197-204
    |
    Published online on: May 6, 2016
       https://doi.org/10.3892/or.2016.4789
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Abstract

Regulated intramembrane proteolysis of epithelial cell adhesion molecule (EpCAM) results in shedding of the extracellular domain (EpEX) and release of the intra­cellular domain (EpICD) into the cytoplasm. Released EpICD associates with FHL2, β-catenin and Lef-1 to form a nuclear complex and triggers oncogenic signaling. This study was conducted to examine the nuclear expression of EpICD in hepatocellular carcinoma (HCC) and to assess the role of EpICD in HCC. EpICD immunoexpression was examined in 100 cases of HCC using tissue microarrays and correlated with clinicopathological parameters. We also examined the role of EpICD in HCC using EpICD cDNA transfected HCC cell line and EpCAM silenced HCC cell line by small interfering RNA (siRNA). Nuclear expression of EpICD was observed in 19 of 100 (19%) cases. Nuclear expression of EpICD significantly correlated with nuclear expression of β-catenin, and Ki-67 labeling index. In addition, nuclear expression of EpICD was associated with higher histologic grade and advanced T category. Forced overexpression of EpICD in the HCC cell significantly increased the cell proliferation, migration and invasion. The overexpression of EpICD also increased the expression levels of the active form of β-catenin and c-myc and cyclin D1. In contrast, downregulation of EpCAM by siRNA decreased the cell proliferation, migration, invasion and the expression of active form of β-catenin, c-myc and cyclin D1. Our present data suggest that EpICD plays important roles in HCC progression by modulating expression of target genes of EpCAM.
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Copy and paste a formatted citation
Spandidos Publications style
Park SY, Bae JS, Cha EJ, Chu HH, Sohn JS and Moon WS: Nuclear EpICD expression and its role in hepatocellular carcinoma. Oncol Rep 36: 197-204, 2016.
APA
Park, S.Y., Bae, J.S., Cha, E.J., Chu, H.H., Sohn, J.S., & Moon, W.S. (2016). Nuclear EpICD expression and its role in hepatocellular carcinoma. Oncology Reports, 36, 197-204. https://doi.org/10.3892/or.2016.4789
MLA
Park, S. Y., Bae, J. S., Cha, E. J., Chu, H. H., Sohn, J. S., Moon, W. S."Nuclear EpICD expression and its role in hepatocellular carcinoma". Oncology Reports 36.1 (2016): 197-204.
Chicago
Park, S. Y., Bae, J. S., Cha, E. J., Chu, H. H., Sohn, J. S., Moon, W. S."Nuclear EpICD expression and its role in hepatocellular carcinoma". Oncology Reports 36, no. 1 (2016): 197-204. https://doi.org/10.3892/or.2016.4789
Copy and paste a formatted citation
x
Spandidos Publications style
Park SY, Bae JS, Cha EJ, Chu HH, Sohn JS and Moon WS: Nuclear EpICD expression and its role in hepatocellular carcinoma. Oncol Rep 36: 197-204, 2016.
APA
Park, S.Y., Bae, J.S., Cha, E.J., Chu, H.H., Sohn, J.S., & Moon, W.S. (2016). Nuclear EpICD expression and its role in hepatocellular carcinoma. Oncology Reports, 36, 197-204. https://doi.org/10.3892/or.2016.4789
MLA
Park, S. Y., Bae, J. S., Cha, E. J., Chu, H. H., Sohn, J. S., Moon, W. S."Nuclear EpICD expression and its role in hepatocellular carcinoma". Oncology Reports 36.1 (2016): 197-204.
Chicago
Park, S. Y., Bae, J. S., Cha, E. J., Chu, H. H., Sohn, J. S., Moon, W. S."Nuclear EpICD expression and its role in hepatocellular carcinoma". Oncology Reports 36, no. 1 (2016): 197-204. https://doi.org/10.3892/or.2016.4789
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