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Article

Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway

  • Authors:
    • Pai-Yun Li
    • Jing Lv
    • Wei-Wei Qi
    • Shu-Fen Zhao
    • Li-Bin Sun
    • Ning Liu
    • Jie Sheng
    • Wen-Sheng Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
  • Pages: 448-454
    |
    Published online on: May 13, 2016
       https://doi.org/10.3892/or.2016.4805
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Abstract

Tetraspanins are a heterogeneous group of 4-transmembrane proteins that recruit other cell surface receptors and signaling proteins into tetraspanin-enriched microdomains (TEMs). TEMs of various types are involved in the regulation of cell growth, migration and invasion of several tumor cell types, both as suppressors or promotors. Tetraspanin 9 (Tspan9, NET-5, PP1057), a member of the transmembrane 4 superfamily (TM4SF) of tetraspanins, reportedly regulates platelet function in concert with other platelet tetraspanins and their associated proteins. Our previous study demonstrated that Tspan9 is also expressed in gastric cancer (GC), but the role of Tspan9 in GC has not been well characterized. In this study, we investigated the influence of Tspan9 on proliferation, migration and invasion of human gastric cancer SGC7901 cells using CCK-8 assay, cell cycle analysis, wound-healing assay and Transwell assay. Western blot analysis and ELISA assay were also performed to identify the potential mechanisms involved. The proliferation, migration and invasion of human gastric cancer SGC7901 cells were significantly inhibited by overexpression of Tspan9. In addition, Tspan9 downregulated the phosphorylation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and the secretion levels of proteins related to tumor metastasis, such as matrix metalloproteinase-9 (MMP-9) and urokinase plasminogen activator (uPA). Our study indicated that Tspan9 inhibited SGC7901 cell proliferation, migration and invasion through the ERK1/2 pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li P, Lv J, Qi W, Zhao S, Sun L, Liu N, Sheng J and Qiu W: Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway. Oncol Rep 36: 448-454, 2016.
APA
Li, P., Lv, J., Qi, W., Zhao, S., Sun, L., Liu, N. ... Qiu, W. (2016). Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway. Oncology Reports, 36, 448-454. https://doi.org/10.3892/or.2016.4805
MLA
Li, P., Lv, J., Qi, W., Zhao, S., Sun, L., Liu, N., Sheng, J., Qiu, W."Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway". Oncology Reports 36.1 (2016): 448-454.
Chicago
Li, P., Lv, J., Qi, W., Zhao, S., Sun, L., Liu, N., Sheng, J., Qiu, W."Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway". Oncology Reports 36, no. 1 (2016): 448-454. https://doi.org/10.3892/or.2016.4805
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Lv J, Qi W, Zhao S, Sun L, Liu N, Sheng J and Qiu W: Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway. Oncol Rep 36: 448-454, 2016.
APA
Li, P., Lv, J., Qi, W., Zhao, S., Sun, L., Liu, N. ... Qiu, W. (2016). Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway. Oncology Reports, 36, 448-454. https://doi.org/10.3892/or.2016.4805
MLA
Li, P., Lv, J., Qi, W., Zhao, S., Sun, L., Liu, N., Sheng, J., Qiu, W."Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway". Oncology Reports 36.1 (2016): 448-454.
Chicago
Li, P., Lv, J., Qi, W., Zhao, S., Sun, L., Liu, N., Sheng, J., Qiu, W."Tspan9 inhibits the proliferation, migration and invasion of human gastric cancer SGC7901 cells via the ERK1/2 pathway". Oncology Reports 36, no. 1 (2016): 448-454. https://doi.org/10.3892/or.2016.4805
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