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Article

Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells

  • Authors:
    • Jia Wang
    • Xianyao Wan
    • Yifan Gao
    • Mintao Zhong
    • Li Sha
    • Ben Liu
    • Wei Zhang
    • Li Tian
    • Wenjing Ruan
    • Shuyun Cao
    • Min Huang
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116021, P.R. China, Department of Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China
  • Pages: 441-447
    |
    Published online on: May 23, 2016
       https://doi.org/10.3892/or.2016.4830
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Abstract

Latcripin-13 domain, isolated from the transcriptome of Lentinula edodes C91-3, contains a regulator of chromosome condensation (RCC1) domain/β-lactamase-inhibitor protein II (BLIP-II) and a plant homeodomain (PHD). Latcripin-13 domain has been shown to have antitumor effects. However, the underlying molecular pharmacology is largely unknown. We report here that Latcripin-13 domain induced cell cycle arrest in the G1 phase and caused the apoptosis of human lung carcinoma A549 cells via the GSK3β-cyclin D1 and caspase-8/NF-κB signaling pathways. Western blot analysis showed that Latcripin-13 domain decreased cyclin D1 and cyclin-dependent kinase 4 (CDK4), while it increased the ratio of GSK3β/phosphorylated GSK3β. Importantly, Latcripin-13 domain induced nuclear fragmentation and chromatin condensation in the A549 cells. In addition, treatment of the A549 cells with Latcripin-13 domain resulted in the loss of mitochondrial membrane potential, accompanied by an increase in the Bax/Bcl-2 ratio and activation of caspase-3, -8, and -9. Intriguingly, western blot analysis revealed that NF-κB was significantly downregulated by Latcripin-13 domain. These results demonstrated that Latcripin-13 domain induced apoptosis and cell cycle arrest at G1 phase in the A549 cells, providing a mechanism for the antitumor effects of Latcripin-13 domain.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Wan X, Gao Y, Zhong M, Sha L, Liu B, Zhang W, Tian L, Ruan W, Cao S, Cao S, et al: Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells. Oncol Rep 36: 441-447, 2016.
APA
Wang, J., Wan, X., Gao, Y., Zhong, M., Sha, L., Liu, B. ... Huang, M. (2016). Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells. Oncology Reports, 36, 441-447. https://doi.org/10.3892/or.2016.4830
MLA
Wang, J., Wan, X., Gao, Y., Zhong, M., Sha, L., Liu, B., Zhang, W., Tian, L., Ruan, W., Cao, S., Huang, M."Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells". Oncology Reports 36.1 (2016): 441-447.
Chicago
Wang, J., Wan, X., Gao, Y., Zhong, M., Sha, L., Liu, B., Zhang, W., Tian, L., Ruan, W., Cao, S., Huang, M."Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells". Oncology Reports 36, no. 1 (2016): 441-447. https://doi.org/10.3892/or.2016.4830
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Wan X, Gao Y, Zhong M, Sha L, Liu B, Zhang W, Tian L, Ruan W, Cao S, Cao S, et al: Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells. Oncol Rep 36: 441-447, 2016.
APA
Wang, J., Wan, X., Gao, Y., Zhong, M., Sha, L., Liu, B. ... Huang, M. (2016). Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells. Oncology Reports, 36, 441-447. https://doi.org/10.3892/or.2016.4830
MLA
Wang, J., Wan, X., Gao, Y., Zhong, M., Sha, L., Liu, B., Zhang, W., Tian, L., Ruan, W., Cao, S., Huang, M."Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells". Oncology Reports 36.1 (2016): 441-447.
Chicago
Wang, J., Wan, X., Gao, Y., Zhong, M., Sha, L., Liu, B., Zhang, W., Tian, L., Ruan, W., Cao, S., Huang, M."Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells". Oncology Reports 36, no. 1 (2016): 441-447. https://doi.org/10.3892/or.2016.4830
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