Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells

Wang,Jia; Wan,Xianyao; Gao,Yifan; Zhong,Mintao; Sha,Li; Liu,Ben; Zhang,Wei; Tian,Li; Ruan,Wenjing; Cao,Shuyun; Huang,Min

doi:10.3892/or.2016.4830

Latcripin-13 domain induces apoptosis and cell cycle arrest at the G1 phase in human lung carcinoma A549 cells

Authors:
- Jia Wang
- Xianyao Wan
- Yifan Gao
- Mintao Zhong
- Li Sha
- Ben Liu
- Wei Zhang
- Li Tian
- Wenjing Ruan
- Shuyun Cao
- Min Huang
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Affiliations: Department of Critical Care Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116021, P.R. China, Department of Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China
Published online on: May 23, 2016 https://doi.org/10.3892/or.2016.4830
Pages: 441-447

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Abstract

Latcripin-13 domain, isolated from the transcriptome of Lentinula edodes C91-3, contains a regulator of chromosome condensation (RCC1) domain/β-lactamase-inhibitor protein II (BLIP-II) and a plant homeodomain (PHD). Latcripin-13 domain has been shown to have antitumor effects. However, the underlying molecular pharmacology is largely unknown. We report here that Latcripin-13 domain induced cell cycle arrest in the G1 phase and caused the apoptosis of human lung carcinoma A549 cells via the GSK3β-cyclin D1 and caspase-8/NF-κB signaling pathways. Western blot analysis showed that Latcripin-13 domain decreased cyclin D1 and cyclin-dependent kinase 4 (CDK4), while it increased the ratio of GSK3β/phosphorylated GSK3β. Importantly, Latcripin-13 domain induced nuclear fragmentation and chromatin condensation in the A549 cells. In addition, treatment of the A549 cells with Latcripin-13 domain resulted in the loss of mitochondrial membrane potential, accompanied by an increase in the Bax/Bcl-2 ratio and activation of caspase-3, -8, and -9. Intriguingly, western blot analysis revealed that NF-κB was significantly downregulated by Latcripin-13 domain. These results demonstrated that Latcripin-13 domain induced apoptosis and cell cycle arrest at G1 phase in the A549 cells, providing a mechanism for the antitumor effects of Latcripin-13 domain.

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