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Article

High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation

  • Authors:
    • Changlei Su
    • Bomiao Zhang
    • Wenzhi Liu
    • Hongqun Zheng
    • Lingyu Sun
    • Jinxue Tong
    • Tian Wang
    • Xiaofeng Jiang
    • Hongyan Liang
    • Li Xue
    • Qifan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Hospital of Harbin Medical University, Harbin 150001, P.R. China, Department of Colorectal Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150040, P.R. China, College of Automation, Harbin Engineering University, Harbin 150001, P.R. China, Department of Surgical Oncology, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China, Clinical Laboratory, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China
  • Pages: 1048-1054
    |
    Published online on: May 30, 2016
       https://doi.org/10.3892/or.2016.4841
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Abstract

Slightly increased pressure stimulates tumor cell adhesion and proliferation. In the present study, we aimed to evaluate the effects of high pressure on gene expression and the biological behavior of gastric cancer cells. After incubation for 30 min at 37˚C under ambient and increased pressure, one portion of SGC7901 cells was used for cell proliferation and apoptosis assays, cell cycle analysis, adhesion invasion or migration assays. The other portion of cells was harvested for detection of matrix metalloproteinase-2 (MMP-2), inhibitor of DNA binding-1 (ID1), sonic Hedgehog (SHH) and E-cadherin expression by western blotting or RT-PCR. In addition, we investigated the effects of high pressure on SGC7901 cell ultrastructure by transmission electron microscopy. We found that the adhesion fold under increased pressure of 760 and 1,520 mmHg was 2.39±1.05 (P<0.05) and 2.47±0.85 (P<0.01) as compared with the control, respectively. The invasion fold was 3.42±2.06 (P<0.05) and 5.13±2.49 (P<0.01) as compared with the control, respectively. The migration was 1.65±0.20 (P<0.001) and 2.53±0.50 (P<0.001) as compared with the control, respectively. At increased pressure, MMP-2 and ID1 expression increased significantly, while the expression of SHH decreased significantly. However, we did not find significant change in proliferation, apoptosis, cell cycle or ultrastructure of the SGC7901 cells under high pressure. In conclusion, high pressure promoted the adhesion, invasion and migration of SGC7901 cells. Moreover, the present study suggests that the pressure-augmented invasion and migration may be related to the increase in MMP-2 expression. Moreover, high pressure may suppress SGC7901 cell differentiation, which may result from the change in SHH and ID1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Su C, Zhang B, Liu W, Zheng H, Sun L, Tong J, Wang T, Jiang X, Liang H, Xue L, Xue L, et al: High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation. Oncol Rep 36: 1048-1054, 2016.
APA
Su, C., Zhang, B., Liu, W., Zheng, H., Sun, L., Tong, J. ... Zhang, Q. (2016). High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation. Oncology Reports, 36, 1048-1054. https://doi.org/10.3892/or.2016.4841
MLA
Su, C., Zhang, B., Liu, W., Zheng, H., Sun, L., Tong, J., Wang, T., Jiang, X., Liang, H., Xue, L., Zhang, Q."High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation". Oncology Reports 36.2 (2016): 1048-1054.
Chicago
Su, C., Zhang, B., Liu, W., Zheng, H., Sun, L., Tong, J., Wang, T., Jiang, X., Liang, H., Xue, L., Zhang, Q."High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation". Oncology Reports 36, no. 2 (2016): 1048-1054. https://doi.org/10.3892/or.2016.4841
Copy and paste a formatted citation
x
Spandidos Publications style
Su C, Zhang B, Liu W, Zheng H, Sun L, Tong J, Wang T, Jiang X, Liang H, Xue L, Xue L, et al: High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation. Oncol Rep 36: 1048-1054, 2016.
APA
Su, C., Zhang, B., Liu, W., Zheng, H., Sun, L., Tong, J. ... Zhang, Q. (2016). High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation. Oncology Reports, 36, 1048-1054. https://doi.org/10.3892/or.2016.4841
MLA
Su, C., Zhang, B., Liu, W., Zheng, H., Sun, L., Tong, J., Wang, T., Jiang, X., Liang, H., Xue, L., Zhang, Q."High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation". Oncology Reports 36.2 (2016): 1048-1054.
Chicago
Su, C., Zhang, B., Liu, W., Zheng, H., Sun, L., Tong, J., Wang, T., Jiang, X., Liang, H., Xue, L., Zhang, Q."High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation". Oncology Reports 36, no. 2 (2016): 1048-1054. https://doi.org/10.3892/or.2016.4841
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