Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line

  • Authors:
    • Caixia Wang
    • Wenjuan Xu
    • Wenjin Hao
    • Bingsheng Wang
    • Qiusheng Zheng
  • View Affiliations

  • Published online on: June 2, 2016     https://doi.org/10.3892/or.2016.4844
  • Pages: 1150-1156
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

High malignant potential and low susceptibility to treatment are characteristics of malignant melanoma. Alternol, a novel compound purified from microbial fermentation products obtained from the bark of the yew tree, exhibits a variety of antitumor activities. Based on these findings, the aim of the present study was to extend the knowledge on the antineoplastic effect of alternol in the mouse melanoma B16F0 cell line. Alternol significantly inhibited the proliferation and colony formation of B16F0 cells in a dose-dependent manner as detected by MTT and soft agar colony formation assays. NaOH alkaline lysis and oxidation of Dopa indicated that alternol enhanced the melanin content and tyrosinase activity of the B16F0 cells and results also showed a dose‑response relationship. Morphologic changes accompanied by extended dendrites were discovered in the B16F0 cells after treatment with alternol. Furthermore, the mRNA levels of tyrosinase, Trp1 and Trp2 were increased by alternol. Our results confirmed that alternol possesses marked antineoplastic properties against melanoma cells, indicating that this microbial fermentation product is a promising agent for the differentiation therapy of cancer. The inhibition of cell proliferation and colony formation by alternol was associated with both cytotoxicity and induction of differentiation.
View Figures
View References

Related Articles

Journal Cover

August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Xu W, Hao W, Wang B and Zheng Q: Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line. Oncol Rep 36: 1150-1156, 2016
APA
Wang, C., Xu, W., Hao, W., Wang, B., & Zheng, Q. (2016). Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line. Oncology Reports, 36, 1150-1156. https://doi.org/10.3892/or.2016.4844
MLA
Wang, C., Xu, W., Hao, W., Wang, B., Zheng, Q."Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line". Oncology Reports 36.2 (2016): 1150-1156.
Chicago
Wang, C., Xu, W., Hao, W., Wang, B., Zheng, Q."Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line". Oncology Reports 36, no. 2 (2016): 1150-1156. https://doi.org/10.3892/or.2016.4844