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Article

TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression

  • Authors:
    • Naiwen Zhang
    • Xiaojun Bi
    • Yu Zeng
    • Yuyan Zhu
    • Zhe Zhang
    • Yang Liu
    • Jianfeng Wang
    • Xuejie Li
    • Jianbin Bi
    • Chuize Kong
  • View Affiliations / Copyright

    Affiliations: Institute of Urology, Department of Urology, The First Affiliated Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China
  • Pages: 977-983
    |
    Published online on: June 21, 2016
       https://doi.org/10.3892/or.2016.4889
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Abstract

Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine that is reported to regulate cellular motility and invasive capability during tumor progression. Fascin1, an actin-bundling protein, increases cell motility, migration and adhesion. To investigate the function of TGF-β1 and test whether fascin1 is an important mediator of the tumor response to TGF-β1 in bladder carcinoma cells, real-time RT-PCR and western blot analysis were used to test changes in fascin1 expression after TGF-β1 (10 ng/ml) treatment in T24 and BIU87 cells. Small interfering RNA (siRNA) technique was performed to silence fascin1. Cell viability and biological behavior changes were evaluated by cell growth (MTT), wound-healing and Matrigel invasion assays. In the present study, we found that the mRNA and protein levels of fascin1 in the T24 and BIU87 cells were significantly increased after 10 ng/ml TGF-β1 treatment (p<0.05). The proliferation of T24 cells (p=0.005) was also significantly increased, while no significant change was observed in BIU87 cells (p=0.318). In addition, the migratory and invasive potential of the two cell lines were promoted. Furthermore, we successfully silenced fascin1, and observed that fascin1 siRNA significantly attenuated the migration and invasiveness induced by TGF-β1. The findings suggested that TGF-β1 can promote invasion and migration of T24 and BIU87 bladder carcinoma cells, and the increase in fascin1 expression may be the key point of this impact of TGF-β1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang N, Bi X, Zeng Y, Zhu Y, Zhang Z, Liu Y, Wang J, Li X, Bi J, Kong C, Kong C, et al: TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression. Oncol Rep 36: 977-983, 2016.
APA
Zhang, N., Bi, X., Zeng, Y., Zhu, Y., Zhang, Z., Liu, Y. ... Kong, C. (2016). TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression. Oncology Reports, 36, 977-983. https://doi.org/10.3892/or.2016.4889
MLA
Zhang, N., Bi, X., Zeng, Y., Zhu, Y., Zhang, Z., Liu, Y., Wang, J., Li, X., Bi, J., Kong, C."TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression". Oncology Reports 36.2 (2016): 977-983.
Chicago
Zhang, N., Bi, X., Zeng, Y., Zhu, Y., Zhang, Z., Liu, Y., Wang, J., Li, X., Bi, J., Kong, C."TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression". Oncology Reports 36, no. 2 (2016): 977-983. https://doi.org/10.3892/or.2016.4889
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang N, Bi X, Zeng Y, Zhu Y, Zhang Z, Liu Y, Wang J, Li X, Bi J, Kong C, Kong C, et al: TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression. Oncol Rep 36: 977-983, 2016.
APA
Zhang, N., Bi, X., Zeng, Y., Zhu, Y., Zhang, Z., Liu, Y. ... Kong, C. (2016). TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression. Oncology Reports, 36, 977-983. https://doi.org/10.3892/or.2016.4889
MLA
Zhang, N., Bi, X., Zeng, Y., Zhu, Y., Zhang, Z., Liu, Y., Wang, J., Li, X., Bi, J., Kong, C."TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression". Oncology Reports 36.2 (2016): 977-983.
Chicago
Zhang, N., Bi, X., Zeng, Y., Zhu, Y., Zhang, Z., Liu, Y., Wang, J., Li, X., Bi, J., Kong, C."TGF-β1 promotes the migration and invasion of bladder carcinoma cells by increasing fascin1 expression". Oncology Reports 36, no. 2 (2016): 977-983. https://doi.org/10.3892/or.2016.4889
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