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Article

Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells

  • Authors:
    • Li Liang
    • Jiean Huang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
  • Pages: 1333-1338
    |
    Published online on: July 11, 2016
       https://doi.org/10.3892/or.2016.4927
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Abstract

Oxymatrine, a traditional Chinese herb extracted from Sophora flavescens Ait., displays strong anti-inflammatory and anticancer activities, but how oxymatrine exhibits anticarcinogenic effects in human colorectal cancer (CRC) remains uncertain. The present study aimed to elucidate the exact mechanism by which oxymatrine exhibits anticarcinogenic effects in CRC using the human colon cancer RKO cell line as the experimental model. CRC cells were treated with oxymatrine, and cell proliferation, migration and invasion were examined by colorimetric MTT, Transwell chamber and wound healing assays, respectively. In addition, epithelial-mesenchymal transition (EMT) markers and p65 were assessed by western blot analysis. Our study demonstrated that oxymatrine hindered the proliferation, migration and invasion of the CRC cells. Mechanistically, we found that oxymatrine modulated the expression of EMT markers including E-cadherin, Snail and N-cadherin, and reduced expression of p65 which is crucial to NF-κB activation. In conclusion, our results indicate that oxymatrine reduces the activation of the NF-κB signaling pathway and inhibits CRC invasion by modulating EMT.
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Copy and paste a formatted citation
Spandidos Publications style
Liang L and Huang J: Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells. Oncol Rep 36: 1333-1338, 2016.
APA
Liang, L., & Huang, J. (2016). Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells. Oncology Reports, 36, 1333-1338. https://doi.org/10.3892/or.2016.4927
MLA
Liang, L., Huang, J."Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells". Oncology Reports 36.3 (2016): 1333-1338.
Chicago
Liang, L., Huang, J."Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells". Oncology Reports 36, no. 3 (2016): 1333-1338. https://doi.org/10.3892/or.2016.4927
Copy and paste a formatted citation
x
Spandidos Publications style
Liang L and Huang J: Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells. Oncol Rep 36: 1333-1338, 2016.
APA
Liang, L., & Huang, J. (2016). Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells. Oncology Reports, 36, 1333-1338. https://doi.org/10.3892/or.2016.4927
MLA
Liang, L., Huang, J."Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells". Oncology Reports 36.3 (2016): 1333-1338.
Chicago
Liang, L., Huang, J."Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells". Oncology Reports 36, no. 3 (2016): 1333-1338. https://doi.org/10.3892/or.2016.4927
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