CXCR3A contributes to the invasion and metastasis of gastric cancer cells

  • Authors:
    • Chenggang Yang
    • Wanlei Zheng
    • Wenfeng Du
  • View Affiliations

  • Published online on: July 19, 2016     https://doi.org/10.3892/or.2016.4953
  • Pages: 1686-1692
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Abstract

CXCR3, belonging to CXC chemokine receptors, has been identified to be overexpressed in various kinds of tumors. There are three mRNA variants of CXCR3 (CXCR3A, CXCR3B and CXCR3alt) in human cells. The functions of major CXCR3 isoforms (CXCR3A, CXCR3B) have been reported in some tumors including prostate and breast cancer. However, the effects of CXCR3A and CXCR3B on gastric cancer cell progression remain unknown. The present investigation found that CXCR3A mRNA level was upregulated but CXCR3B mRNA level was downregulated in gastric cancer cells and tissues. In vitro growth analysis showed that CXCR3A acted as a positive mediator in regulating cell growth, whereas CXCR3B exerted the opposite effect. In vitro invasion and migration assays showed that CXCL10 promoted gastric cancer cell invasion and migration via CXCR3A, but not CXCR3B. Moreover, knockdown of CXCR3A inhibited cell growth and metastasis in vivo. Additionally, CXCR3A knockdown attenuated matrix metalloproteinase (MMP)‑13 and IL‑6 expression, and reduced ERK1/2 activation. Together, these data suggest that CXCR3A contributes to the growth, invasion and metastasis of gastric cancer cells in vitro and in vivo, and thus may be a key mediator of gastric cancer progression.
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September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang C, Zheng W and Du W: CXCR3A contributes to the invasion and metastasis of gastric cancer cells. Oncol Rep 36: 1686-1692, 2016
APA
Yang, C., Zheng, W., & Du, W. (2016). CXCR3A contributes to the invasion and metastasis of gastric cancer cells. Oncology Reports, 36, 1686-1692. https://doi.org/10.3892/or.2016.4953
MLA
Yang, C., Zheng, W., Du, W."CXCR3A contributes to the invasion and metastasis of gastric cancer cells". Oncology Reports 36.3 (2016): 1686-1692.
Chicago
Yang, C., Zheng, W., Du, W."CXCR3A contributes to the invasion and metastasis of gastric cancer cells". Oncology Reports 36, no. 3 (2016): 1686-1692. https://doi.org/10.3892/or.2016.4953