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Article

Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells

  • Authors:
    • Wei Xiong
    • Depei Gao
    • Yunfeng Li
    • Xin Liu
    • Peiling Dai
    • Jiyong Qin
    • Guanshun Wang
    • Kangming Li
    • Han Bai
    • Wenhui Li
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Department of Medical Imaging, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Department of Colorectal Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China
  • Pages: 2142-2150
    |
    Published online on: August 12, 2016
       https://doi.org/10.3892/or.2016.5022
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Abstract

Alternative splicing is a key mechanism that regulates protein diversity and has been found to be associated with colon cancer progression and metastasis. However, the function of alternative splicing in chemoradiation‑resistant colon cancer remains elusive. In this study, we constructed a chemoradiation‑resistant colon cancer cell line. Through RNA-sequencing of normal and chemoradiation‑resistant colon cancer cells (HCT116), we found 818 genes that were highly expressed in the normal HCT116 cells, whereas 285 genes were highly expressed in the chemoradiation-resistant HCT116 (RCR-HCT116) cells. Gene ontology (GO) analysis showed that genes that were highly expressed in the HCT116 cells were enriched in GO categories related to cell cycle and cell division, whereas genes that were highly expressed in the RCR-HCT116 cells were associated with regulation of system processes and response to wounding. Analysis of alternative splicing events revealed that exon skipping was significantly increased in the chemoradiation‑resistant colon cancer cells. Moreover, we identified 323 alternative splicing events in 293 genes that were significantly different between the two different HCT116 cell types. These alternative splicing‑related genes were clustered functionally into several groups related with DNA replication, such as deoxyribonucleotide metabolic/catabolic processes, response to DNA damage stimulus and helicase activity. These findings enriched our knowledge by elucidating the function of alternative splicing in chemoradiation-resistant colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong W, Gao D, Li Y, Liu X, Dai P, Qin J, Wang G, Li K, Bai H, Li W, Li W, et al: Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells. Oncol Rep 36: 2142-2150, 2016.
APA
Xiong, W., Gao, D., Li, Y., Liu, X., Dai, P., Qin, J. ... Li, W. (2016). Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells. Oncology Reports, 36, 2142-2150. https://doi.org/10.3892/or.2016.5022
MLA
Xiong, W., Gao, D., Li, Y., Liu, X., Dai, P., Qin, J., Wang, G., Li, K., Bai, H., Li, W."Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells". Oncology Reports 36.4 (2016): 2142-2150.
Chicago
Xiong, W., Gao, D., Li, Y., Liu, X., Dai, P., Qin, J., Wang, G., Li, K., Bai, H., Li, W."Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells". Oncology Reports 36, no. 4 (2016): 2142-2150. https://doi.org/10.3892/or.2016.5022
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong W, Gao D, Li Y, Liu X, Dai P, Qin J, Wang G, Li K, Bai H, Li W, Li W, et al: Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells. Oncol Rep 36: 2142-2150, 2016.
APA
Xiong, W., Gao, D., Li, Y., Liu, X., Dai, P., Qin, J. ... Li, W. (2016). Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells. Oncology Reports, 36, 2142-2150. https://doi.org/10.3892/or.2016.5022
MLA
Xiong, W., Gao, D., Li, Y., Liu, X., Dai, P., Qin, J., Wang, G., Li, K., Bai, H., Li, W."Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells". Oncology Reports 36.4 (2016): 2142-2150.
Chicago
Xiong, W., Gao, D., Li, Y., Liu, X., Dai, P., Qin, J., Wang, G., Li, K., Bai, H., Li, W."Genome-wide profiling of chemoradiation‑induced changes in alternative splicing in colon cancer cells". Oncology Reports 36, no. 4 (2016): 2142-2150. https://doi.org/10.3892/or.2016.5022
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