MicroRNA-330-5p negatively regulates ITGA5 expression in human colorectal cancer

Yoo,Hye-In; Kim,Bong-Kyu; Yoon,Sungjoo  Kim

doi:10.3892/or.2016.5092

MicroRNA-330-5p negatively regulates ITGA5 expression in human colorectal cancer

Authors:
- Hye-In Yoo
- Bong-Kyu Kim
- Sungjoo Kim Yoon
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Affiliations: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Seochogu 137-701, Republic of Korea
Published online on: September 15, 2016 https://doi.org/10.3892/or.2016.5092
Pages: 3023-3029

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Abstract

Colorectal cancer (CRC), one of the most prevalent malignant cancers, has high rates pf incidence and is the fourth leading cause of cancer-related deaths for both men and women worldwide. MicroRNAs (miRNAs) play critical roles in the development of various types of cancers. miRNA‑330-5p has been implicated in the progression of prostate, neuronal and pancreatic cancers by regulating proliferation, migration, invasion and epithelial-mesenchymal transition of cells. The purpose of the present study was to investigate the expression of miR-330-5p in CRC and identify its target gene(s) that may act in CRC tumorigenesis. We found that miR-330-5p expression was significantly lower in CRC tissues than that in adjacent non-tumorous tissues. Furthermore, we identified integrin α5 (ITGA5) as a new target of miR-330-5p and found that it inhibits ITGA5 expression by directly binding to the 3' untranslated region of ITGA5 mRNA. These results suggest that downregulation of miR-330-5p expression may affect CRC development via modulation of ITGA5 expression.

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