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November-2016 Volume 36 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth

  • Authors:
    • Feng Yan
    • Xiaoming Wang
    • Mingchen Zhu
    • Xiaoya Hu
  • View Affiliations / Copyright

    Affiliations: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, P.R. China, Department of Clinical Laboratory, Nanjing Medical University Cancer Hospital and Jiangsu Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 2793-2799
    |
    Published online on: September 23, 2016
       https://doi.org/10.3892/or.2016.5126
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Abstract

Cyclin Y (CCNY) is a newly identified PFTK1 interacting protein and has been found to be associated with the proliferation and tumorigenesis of human non-small cell lung cancer. In the present study, we analyzed the expression levels of CCNY in 65 cases of breast cancer (BC) tissues and in four BC cell lines, BT-474, MDA-MB-231, T-47D and MCF-7. Lentivirus-mediated short hairpin RNA (shRNA) was employed to knock down CCNY expression in MCF-7 and MDA-MB-231 cells. The effects of CCNY depletion on cell growth were examined by MTT, colony formation and flow cytometry assays. The results showed that immunohistochemical expression of CCNY in tumor tissues is stronger than that in normal tissues. CCNY was also expressed in all four BC cells. The knockdown of CCNY resulted in a significant reduction in cell proliferation and colony formation ability. Cell cycle analysis showed that CCNY knockdown arrested MDA-MB‑231 cells in the G0/G1 phase. Furthermore, depletion of CCNY inhibited BC cell growth via the activation of Bad and GSK3β, as well as cleavages of PARP and caspase-3 in a p53-dependent manner. Therefore, we believe that CCNY has biological effect in BC development, and its inhibition via an RNA interference lentiviral system may provide a therapeutic option for BC.
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Copy and paste a formatted citation
Spandidos Publications style
Yan F, Wang X, Zhu M and Hu X: RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth. Oncol Rep 36: 2793-2799, 2016.
APA
Yan, F., Wang, X., Zhu, M., & Hu, X. (2016). RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth. Oncology Reports, 36, 2793-2799. https://doi.org/10.3892/or.2016.5126
MLA
Yan, F., Wang, X., Zhu, M., Hu, X."RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth". Oncology Reports 36.5 (2016): 2793-2799.
Chicago
Yan, F., Wang, X., Zhu, M., Hu, X."RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth". Oncology Reports 36, no. 5 (2016): 2793-2799. https://doi.org/10.3892/or.2016.5126
Copy and paste a formatted citation
x
Spandidos Publications style
Yan F, Wang X, Zhu M and Hu X: RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth. Oncol Rep 36: 2793-2799, 2016.
APA
Yan, F., Wang, X., Zhu, M., & Hu, X. (2016). RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth. Oncology Reports, 36, 2793-2799. https://doi.org/10.3892/or.2016.5126
MLA
Yan, F., Wang, X., Zhu, M., Hu, X."RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth". Oncology Reports 36.5 (2016): 2793-2799.
Chicago
Yan, F., Wang, X., Zhu, M., Hu, X."RNAi-mediated downregulation of cyclin Y to attenuate human breast cancer cell growth". Oncology Reports 36, no. 5 (2016): 2793-2799. https://doi.org/10.3892/or.2016.5126
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