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Article Open Access

Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas

  • Authors:
    • Lene E. Johannessen
    • Ioannis Panagopoulos
    • Sven-Petter Haugvik
    • Ivar Prydz Gladhaug
    • Sverre Heim
    • Francesca Micci
  • View Affiliations / Copyright

    Affiliations: Section for Cancer Cytogenetics, Institute for Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University Hospital, 0310 Oslo, Norway, Department of Hepato-Pancreato-Biliary Surgery, Rikshospitalet, Oslo University Hospital, 0372 Oslo, Norway
    Copyright: © Johannessen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2653-2662
    |
    Published online on: September 23, 2016
       https://doi.org/10.3892/or.2016.5132
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Abstract

Fusion transcripts arising from the combination of exons residing on neighboring genes on the same chromosome may give rise to chimeric or novel proteins. Such read-through transcripts have been detected in different cancers where they may be of pathogenetic interest. In this study, we describe for the first time the expression of a read-through transcript in insulinomas, a functioning neuroendocrine pancreatic neoplasm. The read-through transcript INS-IGF2, composed of exons from the two genes proinsulin precursor (INS) and insulin‑like growth factor 2 (IGF2), both mapping to chromosomal subband 11p15.5, was highly expressed in the two insulinomas analyzed. More precisely, version 2 of the INS-IGF2 transcript was expressed, indicating possible expression of the chimeric INS-IGF2 protein. We further identified a novel splice variant of the INS-IGF2 read-through transcript in one of the insulinomas, composed of exon 1 of INS3 and exons of IGF2. In the same tumor, we found high expression of INS3 and the presence of the A allele at SNP rs689. SNP rs689 has been previously described to regulate splicing of the INS transcript, indicating that this regulatory mechanism also affects splicing of INS-IGF2. The identification of the INS-IGF2 read-through transcript specifically in tumor tissue but not in normal pancreatic tissue suggests that high expression of INS-IGF2 could be neoplasia‑specific. These results may have potential clinical applications given that the read-through transcript could be used as a biomarker in insulinoma patients.
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Copy and paste a formatted citation
Spandidos Publications style
Johannessen LE, Panagopoulos I, Haugvik S, Gladhaug IP, Heim S and Micci F: Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas. Oncol Rep 36: 2653-2662, 2016.
APA
Johannessen, L.E., Panagopoulos, I., Haugvik, S., Gladhaug, I.P., Heim, S., & Micci, F. (2016). Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas. Oncology Reports, 36, 2653-2662. https://doi.org/10.3892/or.2016.5132
MLA
Johannessen, L. E., Panagopoulos, I., Haugvik, S., Gladhaug, I. P., Heim, S., Micci, F."Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas". Oncology Reports 36.5 (2016): 2653-2662.
Chicago
Johannessen, L. E., Panagopoulos, I., Haugvik, S., Gladhaug, I. P., Heim, S., Micci, F."Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas". Oncology Reports 36, no. 5 (2016): 2653-2662. https://doi.org/10.3892/or.2016.5132
Copy and paste a formatted citation
x
Spandidos Publications style
Johannessen LE, Panagopoulos I, Haugvik S, Gladhaug IP, Heim S and Micci F: Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas. Oncol Rep 36: 2653-2662, 2016.
APA
Johannessen, L.E., Panagopoulos, I., Haugvik, S., Gladhaug, I.P., Heim, S., & Micci, F. (2016). Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas. Oncology Reports, 36, 2653-2662. https://doi.org/10.3892/or.2016.5132
MLA
Johannessen, L. E., Panagopoulos, I., Haugvik, S., Gladhaug, I. P., Heim, S., Micci, F."Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas". Oncology Reports 36.5 (2016): 2653-2662.
Chicago
Johannessen, L. E., Panagopoulos, I., Haugvik, S., Gladhaug, I. P., Heim, S., Micci, F."Upregulation of INS-IGF2 read-through expression and identification of a novel INS-IGF2 splice variant in insulinomas". Oncology Reports 36, no. 5 (2016): 2653-2662. https://doi.org/10.3892/or.2016.5132
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