MicroRNA-382 inhibits prostate cancer cell proliferation and metastasis through targeting COUP-TFII

  • Authors:
    • Wei Zhang
    • Jianzhou Liu
    • Jianxin Qiu
    • Xiaoliang Fu
    • Qisheng Tang
    • Fan Yang
    • Zhiguang Zhao
    • He Wang
  • View Affiliations

  • Published online on: October 3, 2016     https://doi.org/10.3892/or.2016.5141
  • Pages: 3707-3715
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Abstract

MicroRNAs (miRNAs) have emerged as important regulators in cancer that are implicated in regulation of various cellular processes. miR-382 has been proposed as a tumor suppressor by several recent studies. However, the function of miR-382 in prostate cancer remains unknown. In this study, we aimed to investigate the potential function of miR-382 in prostate cancer. We found that miR-382 was significantly decreased in prostate cancer specimens and cancer cell lines. The overexpression of miR-382 in prostate cancer cells markedly inhibited cell proliferation, migration, and invasion. In contrast, miR-382 suppression exhibited an opposite effect. Target analysis predicted that chicken ovalbumin upstream promoter transcription factor II (COUP‑TFII) was a direct target of miR-382. This prediction was experimentally confirmed by dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and western blot analysis. Our results further demonstrated that miR-382 inhibited the downstream genes of COUP‑TFII, including Snail and matrix metalloproteinase 2 (MMP2). Moreover, the restoration of COUP‑TFII expression significantly blocked the inhibitory effect of miR-382 on cell proliferation, migration, and invasion, and Snail expression. Taken together, this study suggests that miR-382 inhibits prostate cancer cell proliferation and metastasis through inhibiting COUP‑TFII, representing an important new mechanism for understanding prostate cancer pathogenesis and providing a novel therapeutic candidate target for prostate cancer therapy.
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December-2016
Volume 36 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang W, Liu J, Qiu J, Fu X, Tang Q, Yang F, Zhao Z and Wang H: MicroRNA-382 inhibits prostate cancer cell proliferation and metastasis through targeting COUP-TFII . Oncol Rep 36: 3707-3715, 2016
APA
Zhang, W., Liu, J., Qiu, J., Fu, X., Tang, Q., Yang, F. ... Wang, H. (2016). MicroRNA-382 inhibits prostate cancer cell proliferation and metastasis through targeting COUP-TFII . Oncology Reports, 36, 3707-3715. https://doi.org/10.3892/or.2016.5141
MLA
Zhang, W., Liu, J., Qiu, J., Fu, X., Tang, Q., Yang, F., Zhao, Z., Wang, H."MicroRNA-382 inhibits prostate cancer cell proliferation and metastasis through targeting COUP-TFII ". Oncology Reports 36.6 (2016): 3707-3715.
Chicago
Zhang, W., Liu, J., Qiu, J., Fu, X., Tang, Q., Yang, F., Zhao, Z., Wang, H."MicroRNA-382 inhibits prostate cancer cell proliferation and metastasis through targeting COUP-TFII ". Oncology Reports 36, no. 6 (2016): 3707-3715. https://doi.org/10.3892/or.2016.5141