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Article

MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells

  • Authors:
    • Yanke Chen
    • Xiaofei Wang
    • Jiwen Cheng
    • Zhen Wang
    • Ting Jiang
    • Ni Hou
    • Na Liu
    • Tusheng Song
    • Chen Huang
  • View Affiliations / Copyright

    Affiliations: Experiment Center of Biomedical Research, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Genetics and Cell Biology, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China, Departments of Hepatobiliary Surgery of The First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Health Science Center, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 3379-3386
    |
    Published online on: October 4, 2016
       https://doi.org/10.3892/or.2016.5144
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Abstract

Growing evidence indicates that some abnormally expressed microRNAs (miRNAs) influence tumorigenesis and progression. Previous studies reported that miR-20a is among the frequently altered miRNAs in human hepatocellular carcinoma (HCC), but its expression pattern and role in HCC remain controversial. In the present study, we demonstrated that miR-20a-5p exhibited aberrant expression in HCC tissues compared with paired non-tumor tissues: 52% of the tumor samples showed a greater increase. Overexpression of miR-20a contributed to HCC cell proliferation and migration in vitro, and treatment with anti-miR20a-5p caused the opposite effects. Further studies revealed RUNX3, an important tumor-suppressor, as a direct target of miR-20a-5p. We observed that the level of RUNX3 was sharply reduced in both mRNA and protein in HCC tissues compared with paired non-tumor tissues. Collectively, our results support the viewpoint that miR-20-5p has an oncogenic property, miR-20a overexpression contributed to HCC cell proliferation and migration through reducing the translation of RUNX3. The data provide a new mechanism of miR-20a regulating RUNX3 in HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Wang X, Cheng J, Wang Z, Jiang T, Hou N, Liu N, Song T and Huang C: MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells. Oncol Rep 36: 3379-3386, 2016.
APA
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N. ... Huang, C. (2016). MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells. Oncology Reports, 36, 3379-3386. https://doi.org/10.3892/or.2016.5144
MLA
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N., Liu, N., Song, T., Huang, C."MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells". Oncology Reports 36.6 (2016): 3379-3386.
Chicago
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N., Liu, N., Song, T., Huang, C."MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells". Oncology Reports 36, no. 6 (2016): 3379-3386. https://doi.org/10.3892/or.2016.5144
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Wang X, Cheng J, Wang Z, Jiang T, Hou N, Liu N, Song T and Huang C: MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells. Oncol Rep 36: 3379-3386, 2016.
APA
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N. ... Huang, C. (2016). MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells. Oncology Reports, 36, 3379-3386. https://doi.org/10.3892/or.2016.5144
MLA
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N., Liu, N., Song, T., Huang, C."MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells". Oncology Reports 36.6 (2016): 3379-3386.
Chicago
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N., Liu, N., Song, T., Huang, C."MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells". Oncology Reports 36, no. 6 (2016): 3379-3386. https://doi.org/10.3892/or.2016.5144
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