MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells

  • Authors:
    • Yanke Chen
    • Xiaofei Wang
    • Jiwen Cheng
    • Zhen Wang
    • Ting Jiang
    • Ni Hou
    • Na Liu
    • Tusheng Song
    • Chen Huang
  • View Affiliations

  • Published online on: October 4, 2016     https://doi.org/10.3892/or.2016.5144
  • Pages: 3379-3386
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Abstract

Growing evidence indicates that some abnormally expressed microRNAs (miRNAs) influence tumorigenesis and progression. Previous studies reported that miR-20a is among the frequently altered miRNAs in human hepatocellular carcinoma (HCC), but its expression pattern and role in HCC remain controversial. In the present study, we demonstrated that miR-20a-5p exhibited aberrant expression in HCC tissues compared with paired non-tumor tissues: 52% of the tumor samples showed a greater increase. Overexpression of miR-20a contributed to HCC cell proliferation and migration in vitro, and treatment with anti-miR20a-5p caused the opposite effects. Further studies revealed RUNX3, an important tumor-suppressor, as a direct target of miR-20a-5p. We observed that the level of RUNX3 was sharply reduced in both mRNA and protein in HCC tissues compared with paired non-tumor tissues. Collectively, our results support the viewpoint that miR-20-5p has an oncogenic property, miR-20a overexpression contributed to HCC cell proliferation and migration through reducing the translation of RUNX3. The data provide a new mechanism of miR-20a regulating RUNX3 in HCC.
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December-2016
Volume 36 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen Y, Wang X, Cheng J, Wang Z, Jiang T, Hou N, Liu N, Song T and Huang C: MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells. Oncol Rep 36: 3379-3386, 2016
APA
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N. ... Huang, C. (2016). MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells. Oncology Reports, 36, 3379-3386. https://doi.org/10.3892/or.2016.5144
MLA
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N., Liu, N., Song, T., Huang, C."MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells". Oncology Reports 36.6 (2016): 3379-3386.
Chicago
Chen, Y., Wang, X., Cheng, J., Wang, Z., Jiang, T., Hou, N., Liu, N., Song, T., Huang, C."MicroRNA-20a-5p targets RUNX3 to regulate proliferation and migration of human hepatocellular cancer cells". Oncology Reports 36, no. 6 (2016): 3379-3386. https://doi.org/10.3892/or.2016.5144