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Article

Anticancer effect of thalidomide in vitro on human osteosarcoma cells

  • Authors:
    • Jianwei Zhu
    • Ya Yang
    • Sihong Liu
    • Huihua Xu
    • Yong Wu
    • Guiqiang Zhang
    • Yuxuan Wang
    • Yan Wang
    • Yamin Liu
    • Qifeng Guo
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, The Nursing College, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China
  • Pages: 3545-3551
    |
    Published online on: October 11, 2016
       https://doi.org/10.3892/or.2016.5158
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Abstract

Osteosarcoma is a high‑grade malignant tumor frequently found in children and adolescents. Thalidomide has been reported for treatment of various malignancies. Thalidomide was added to osteosarcoma cells and studied by cytotoxicity assay, evaluating apoptosis, cell cycle arrest, mitochondrial membrane potential (ΔΨm), and reactive oxygen species (ROS) levels and the expression of Bcl‑2, Bax, caspase‑3 and NF‑κB. The results showed that thalidomide could inhibit the proliferation of MG‑63 and U2OS cells in a concentration‑ and time‑dependent manner. Morphological changes of apoptosis were also observed. Thalidomide increased the apoptosis rate of MG‑63 cells and induced cell cycle arrest by increasing the number of cells in the G0/G1 phase and decreasing the percentage of S phase in MG‑63 cells. Further investigation showed that a disruption of ΔΨm and upregulation of ROS were induced by thalidomide in high concentration. By western blot analysis, thalidomide resulted in the decreasing expression of Bcl‑2 and NF‑κB, and the increasing expression of Bcl‑2/Bax and caspase‑3. Here, we provide evidence that thalidomide could cause apoptosis in osteosarcoma cells. Taken together, these results indicate that thalidomide could be an antitumor drug in the therapy of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Yang Y, Liu S, Xu H, Wu Y, Zhang G, Wang Y, Wang Y, Liu Y, Guo Q, Guo Q, et al: Anticancer effect of thalidomide in vitro on human osteosarcoma cells. Oncol Rep 36: 3545-3551, 2016.
APA
Zhu, J., Yang, Y., Liu, S., Xu, H., Wu, Y., Zhang, G. ... Guo, Q. (2016). Anticancer effect of thalidomide in vitro on human osteosarcoma cells. Oncology Reports, 36, 3545-3551. https://doi.org/10.3892/or.2016.5158
MLA
Zhu, J., Yang, Y., Liu, S., Xu, H., Wu, Y., Zhang, G., Wang, Y., Wang, Y., Liu, Y., Guo, Q."Anticancer effect of thalidomide in vitro on human osteosarcoma cells". Oncology Reports 36.6 (2016): 3545-3551.
Chicago
Zhu, J., Yang, Y., Liu, S., Xu, H., Wu, Y., Zhang, G., Wang, Y., Wang, Y., Liu, Y., Guo, Q."Anticancer effect of thalidomide in vitro on human osteosarcoma cells". Oncology Reports 36, no. 6 (2016): 3545-3551. https://doi.org/10.3892/or.2016.5158
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Yang Y, Liu S, Xu H, Wu Y, Zhang G, Wang Y, Wang Y, Liu Y, Guo Q, Guo Q, et al: Anticancer effect of thalidomide in vitro on human osteosarcoma cells. Oncol Rep 36: 3545-3551, 2016.
APA
Zhu, J., Yang, Y., Liu, S., Xu, H., Wu, Y., Zhang, G. ... Guo, Q. (2016). Anticancer effect of thalidomide in vitro on human osteosarcoma cells. Oncology Reports, 36, 3545-3551. https://doi.org/10.3892/or.2016.5158
MLA
Zhu, J., Yang, Y., Liu, S., Xu, H., Wu, Y., Zhang, G., Wang, Y., Wang, Y., Liu, Y., Guo, Q."Anticancer effect of thalidomide in vitro on human osteosarcoma cells". Oncology Reports 36.6 (2016): 3545-3551.
Chicago
Zhu, J., Yang, Y., Liu, S., Xu, H., Wu, Y., Zhang, G., Wang, Y., Wang, Y., Liu, Y., Guo, Q."Anticancer effect of thalidomide in vitro on human osteosarcoma cells". Oncology Reports 36, no. 6 (2016): 3545-3551. https://doi.org/10.3892/or.2016.5158
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