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December-2016 Volume 36 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis

  • Authors:
    • Xiu-Bo Sang
    • Kai-Xuan Sun
    • Li-Li Wang
    • Shuo Chen
    • Dan‑Dan Wu
    • Zhi-Hong Zong
    • Yang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Gynecology, The First Affiliated Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China
  • Pages: 3267-3274
    |
    Published online on: October 11, 2016
       https://doi.org/10.3892/or.2016.5164
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Abstract

Cancer stem cells are considered to be the root cause of tumor initiation, metastasis, recurrence and therapeutic resistance. Recent studies have reported that RhoC plays a critical role in regulating cancer stem cells; however, its function in ovarian cancer stem cells (OCSCs) remains unknown. The ovarian cancer cell line A2780, and the paclitaxel-resistant A2780 cell line (A2780-PTX) were obtained. A2780 cells were used to isolate and identify the highly invasive A2780-PM cells, and A2780-PTX cells were used to isolate and identify the highly drug-resistant and highly invasive A2780-PTX-PM cells by Transwell assay. MTT, Transwell and wound healing assays were used to compare the differences in cell proliferation, invasion and migration ability among the four cell lines. Immunofluorescence was used to detect the expression of stem cell markers CD117 and CD133. OCSCs were sorted by flow cytometry. Following si-RhoC transfection of the OCSCs, cell proliferation, drug resistance, invasion and migration ability and RhoC, CD117 and CD133 expression levels were assayed. RT-PCR was used to assess RhoC, CD117, CD133 and matrix metalloproteinase 9 (MMP9) mRNA expression levels. A2780-PM and A2780‑PTX-PM cells exhibited higher cell proliferation, drug resistance, and invasion and migration ability than the A2780 and A2780-PTX cell lines. Furthermore, CD133 and CD117 expression levels were higher in the A2780-PM and A2780‑PTX-PM cells than levels in the A2780 and A2780-PTX cells. Transfection of si-RhoC in OCSCs suppressed the proliferation, drug resistance, invasion, migration and CD117 and CD133 expression levels. Furthermore, the expression levels of RhoC, CD117, CD133, MDR1, and MMP9 mRNA were downregulated in the transfected population. Taken together, our results demonstrated that RhoC downregulation may inhibit the proliferation, drug resistance, invasion and migration of OCSCs, and RhoC may play an important role in the formation of OCSCs.
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1 

Baldwin LA, Huang B, Miller RW, Tucker T, Goodrich ST, Podzielinski I, DeSimone CP, Ueland FR, van Nagell JR and Seamon LG: Ten-year relative survival for epithelial ovarian cancer. Obstet Gynecol. 120:612–618. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Bae T, Weon KY, Lee JW, Eum KH, Kim S and Choi JW: Restoration of paclitaxel resistance by CDK1 intervention in drug-resistant ovarian cancer. Carcinogenesis. 36:1561–1571. 2015.PubMed/NCBI

3 

Yoo YD and Kwon YT: Molecular mechanisms controlling asymmetric and symmetric self-renewal of cancer stem cells. J Anal Sci Technol. 6:282015. View Article : Google Scholar : PubMed/NCBI

4 

Beachy PA, Karhadkar SS and Berman DM: Tissue repair and stem cell renewal in carcinogenesis. Nature. 432:324–331. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Levina V, Marrangoni AM, DeMarco R, Gorelik E and Lokshin AE: Drug-selected human lung cancer stem cells: Cytokine network, tumorigenic and metastatic properties. PLoS One. 3:e30772008. View Article : Google Scholar : PubMed/NCBI

6 

Klonisch T, Wiechec E, Hombach-Klonisch S, Ande SR, Wesselborg S, Schulze-Osthoff K and Los M: Cancer stem cell markers in common cancers - therapeutic implications. Trends Mol Med. 14:450–460. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Li F, Tiede B, Massagué J and Kang Y: Beyond tumorigenesis: Cancer stem cells in metastasis. Cell Res. 17:3–14. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Reya T, Morrison SJ, Clarke MF and Weissman IL: Stem cells, cancer, and cancer stem cells. Nature. 414:105–111. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Vezzoni L and Parmiani G: Limitations of the cancer stem cell theory. Cytotechnology. 58:3–9. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Sahai E and Marshall CJ: RHO-GTPases and cancer. Nat Rev Cancer. 2:133–142. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Hakem A, Sanchez-Sweatman O, You-Ten A, Duncan G, Wakeham A, Khokha R and Mak TW: RhoC is dispensable for embryogenesis and tumor initiation but essential for metastasis. Genes Dev. 19:1974–1979. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Kleer CG, Teknos TN, Islam M, Marcus B, Lee JS, Pan Q, Merajver SD and Rho C: RhoC GTPase expression as a potential marker of lymph node metastasis in squamous cell carcinomas of the head and neck. Clin Cancer Res. 12:4485–4490. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Islam M, Sharma S and Teknos TN: RhoC regulates cancer stem cells in head and neck squamous cell carcinoma by overexpressing IL-6 and phosphorylation of STAT3. PLoS One. 9:e885272014. View Article : Google Scholar : PubMed/NCBI

14 

Rosenthal DT, Zhang J, Bao L, Zhu L, Wu Z, Toy K, Kleer CG and Merajver SD: RhoC impacts the metastatic potential and abundance of breast cancer stem cells. PLoS One. 7:e409792012. View Article : Google Scholar : PubMed/NCBI

15 

Chen S, Chen X, Xiu YL, Sun KX and Zhao Y and Zhao Y: Inhibition of ovarian epithelial carcinoma tumorigenesis and progression by microRNA 106b mediated through the RhoC pathway. PLoS One. 10:e01257142015. View Article : Google Scholar : PubMed/NCBI

16 

Chen X, Chen S, Xiu YL, Sun KX, Zong ZH and Zhao Y: RhoC is a major target of microRNA-93-5P in epithelial ovarian carcinoma tumorigenesis and progression. Mol Cancer. 14:312015. View Article : Google Scholar : PubMed/NCBI

17 

Chen S, Wang J, Gou WF, Xiu YL, Zheng HC, Zong ZH, Takano Y and Zhao Y: The involvement of RhoA and Wnt-5a in the tumorigenesis and progression of ovarian epithelial carcinoma. Int J Mol Sci. 14:24187–24199. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Lukenda A, Dotlic S, Vukojevic N, Saric B, Vranic S and Zarkovic K: Expression and prognostic value of putative cancer stem cell markers CD117 and CD15 in choroidal and ciliary body melanoma. J Clin Pathol. 69:234–239. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Wright MH, Calcagno AM, Salcido CD, Carlson MD, Ambudkar SV and Varticovski L: Brca1 breast tumors contain distinct CD44+/CD24 and CD133+ cells with cancer stem cell characteristics. Breast Cancer Res. 10:R102008. View Article : Google Scholar : PubMed/NCBI

20 

Xie SL, Zhu MG, Chen GF, Wang GY and Lv GY: Effects of Ras homolog gene family, member C gene silencing combined with rapamycin on hepatocellular carcinoma cell growth. Mol Med Rep. 12:5077–5085. 2015.PubMed/NCBI

21 

Mitin N, Rossman KL, Currin R, Anne S, Marshall TW, Bear JE, Bautch VL and Der CJ: The RhoGEF TEM4 regulates endothelial cell migration by suppressing actomyosin contractility. PLoS One. 8:e662602013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sang X, Sun K, Wang L, Chen S, Wu DD, Zong Z and Zhao Y: Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis. Oncol Rep 36: 3267-3274, 2016.
APA
Sang, X., Sun, K., Wang, L., Chen, S., Wu, D., Zong, Z., & Zhao, Y. (2016). Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis. Oncology Reports, 36, 3267-3274. https://doi.org/10.3892/or.2016.5164
MLA
Sang, X., Sun, K., Wang, L., Chen, S., Wu, D., Zong, Z., Zhao, Y."Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis". Oncology Reports 36.6 (2016): 3267-3274.
Chicago
Sang, X., Sun, K., Wang, L., Chen, S., Wu, D., Zong, Z., Zhao, Y."Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis". Oncology Reports 36, no. 6 (2016): 3267-3274. https://doi.org/10.3892/or.2016.5164
Copy and paste a formatted citation
x
Spandidos Publications style
Sang X, Sun K, Wang L, Chen S, Wu DD, Zong Z and Zhao Y: Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis. Oncol Rep 36: 3267-3274, 2016.
APA
Sang, X., Sun, K., Wang, L., Chen, S., Wu, D., Zong, Z., & Zhao, Y. (2016). Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis. Oncology Reports, 36, 3267-3274. https://doi.org/10.3892/or.2016.5164
MLA
Sang, X., Sun, K., Wang, L., Chen, S., Wu, D., Zong, Z., Zhao, Y."Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis". Oncology Reports 36.6 (2016): 3267-3274.
Chicago
Sang, X., Sun, K., Wang, L., Chen, S., Wu, D., Zong, Z., Zhao, Y."Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis". Oncology Reports 36, no. 6 (2016): 3267-3274. https://doi.org/10.3892/or.2016.5164
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