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EpCAM-independent capture of circulating tumor cells with a ‘universal CTC-chip’

  • Authors:
    • Yasuhiro Chikaishi
    • Kazue Yoneda
    • Takashi Ohnaga
    • Fumihiro Tanaka

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  • Published online on: November 8, 2016     https://doi.org/10.3892/or.2016.5235
  • Pages: 77-82
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Abstract

Capture of circulating tumor cells (CTCs), which are shed from the primary tumor site and circulate in the blood, remains a technical challenge. CellSearch® is the only clinically approved CTC detection system, but has provided only modest sensitivity in detecting CTCs mainly because epithelial cell adhesion molecule (EpCAM)-negative tumor cells may not be captured. To achieve more sensitive CTC‑capture, we have developed a novel microfluidic platform, a ʻCTC-chipʼ comprised of light-curable resins that has a unique advantage in that any capture antibody is easily conjugated. In the present study, we showed that EpCAM-negative tumor cells as well as EpCAM-positive cells were captured with the novel ʻuniversal CTC-chipʼ as follows: i) human lung cancer cells (PC-9), with strong EpCAM expression, were efficiently captured with the CTC-chip coated with an anti-EpCAM antibody (with an average capture efficiency of 101% when tumor cells were spiked in phosphate‑buffered saline (PBS) and 88% when spiked in blood); ii) human mesothelioma cells (ACC-MESO-4), with no EpCAM expression but with podoplanin expression, were captured with the CTC-chip coated with an anti-podoplanin antibody (average capture efficiency of 78% when tumor cells were spiked in PBS and 38% when spiked in blood), whereas ACC-MESO-4 cells were not captured with the CTC-chip coated with the anti-EpCAM antibody. These results indicate that the novel ʻCTC-chipʼ can be useful in sensitive EpCAM-independent detection of CTCs, which may provide new insights into personalized medicine.
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January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Journal Metrics
  • Impact Factor: 4.2
  • Ranked Oncology
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Chikaishi Y, Yoneda K, Ohnaga T and Tanaka F: EpCAM-independent capture of circulating tumor cells with a ‘universal CTC-chip’. Oncol Rep 37: 77-82, 2017
APA
Chikaishi, Y., Yoneda, K., Ohnaga, T., & Tanaka, F. (2017). EpCAM-independent capture of circulating tumor cells with a ‘universal CTC-chip’. Oncology Reports, 37, 77-82. https://doi.org/10.3892/or.2016.5235
MLA
Chikaishi, Y., Yoneda, K., Ohnaga, T., Tanaka, F."EpCAM-independent capture of circulating tumor cells with a ‘universal CTC-chip’". Oncology Reports 37.1 (2017): 77-82.
Chicago
Chikaishi, Y., Yoneda, K., Ohnaga, T., Tanaka, F."EpCAM-independent capture of circulating tumor cells with a ‘universal CTC-chip’". Oncology Reports 37, no. 1 (2017): 77-82. https://doi.org/10.3892/or.2016.5235