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Article

miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer

  • Authors:
    • Guanli Huang
    • Xiangjian Chen
    • Yefeng Cai
    • Xiaobo Wang
    • Chungen Xing
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
  • Pages: 571-578
    |
    Published online on: November 28, 2016
       https://doi.org/10.3892/or.2016.5278
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Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in cancer processes. Although miR-20a has been reported to be altered in a range of cancers, the role of miR-20a in colorectal cancer is not fully characterized, and the relationship between miR-20a dysregulation and the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sensitivity is not defined. In the present study, we demonstrated significant upregulation of miR-20a in the serum of colorectal cancer patients, tumor tissues and cell lines by quantitative RT-PCR analysis. Furthermore, we found that the TRAIL-induced apoptosis was associated with the expression level of miR-20a in colorectal cancer. The knockdown of miR-20a by inhibitors increased the antitumor effect of TRAIL via caspase-8 dependent pathway. BID, which is a pro-apoptotic member of the Bcl-2 family, was found to be directly regulated by miR-20a in SW480 cells. The knockdown of miR-20a inhibited the translocation of tBID to the mitochondria, which induced the mitochondrial pathway of apoptosis. Notably, we found that the knockdown of miR-20a also reversed the resistance of TRAIL in established TRAIL-resistant SW480 cells by tBID-mitochondria pathway. Therefore, our results suggest that miR-20a acts as a tumor promoter in colorectal cancer, and the understanding of the miR-20a might be a potential therapeutic target for colorectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Huang G, Chen X, Cai Y, Wang X and Xing C: miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncol Rep 37: 571-578, 2017.
APA
Huang, G., Chen, X., Cai, Y., Wang, X., & Xing, C. (2017). miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncology Reports, 37, 571-578. https://doi.org/10.3892/or.2016.5278
MLA
Huang, G., Chen, X., Cai, Y., Wang, X., Xing, C."miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer". Oncology Reports 37.1 (2017): 571-578.
Chicago
Huang, G., Chen, X., Cai, Y., Wang, X., Xing, C."miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer". Oncology Reports 37, no. 1 (2017): 571-578. https://doi.org/10.3892/or.2016.5278
Copy and paste a formatted citation
x
Spandidos Publications style
Huang G, Chen X, Cai Y, Wang X and Xing C: miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncol Rep 37: 571-578, 2017.
APA
Huang, G., Chen, X., Cai, Y., Wang, X., & Xing, C. (2017). miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncology Reports, 37, 571-578. https://doi.org/10.3892/or.2016.5278
MLA
Huang, G., Chen, X., Cai, Y., Wang, X., Xing, C."miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer". Oncology Reports 37.1 (2017): 571-578.
Chicago
Huang, G., Chen, X., Cai, Y., Wang, X., Xing, C."miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer". Oncology Reports 37, no. 1 (2017): 571-578. https://doi.org/10.3892/or.2016.5278
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