Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
February-2017 Volume 37 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2017 Volume 37 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β

  • Authors:
    • Sai Li
    • Jincheng Li
    • Tian Hu
    • Chuhong Zhang
    • Xiu Lv
    • Sha He
    • Hanxing Yan
    • Yixi Tan
    • Meiling Wen
    • Mingsheng Lei
    • Jianhong Zuo
  • View Affiliations / Copyright

    Affiliations: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China, Medical School, Shaoyang University, Shaoyang, Hunan 422000, P.R. China, Department of Respiratory and Critical Care Medicine, The People's Hospital of Zhangjiajie City, Zhangjiajie, Hunan 427000, P.R. China
  • Pages: 849-856
    |
    Published online on: December 7, 2016
       https://doi.org/10.3892/or.2016.5295
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Laryngeal carcinoma (LC) is one of the most common malignant tumors of all head and neck squamous cell carcinomas (HNSCCs). However, the molecular mechanism and genetic basis of the development of LC have not been fully elucidated. To explore the possible mechanism, targeted proteomic analysis was performed on Bcl-2-associated proteins from LC cells. According to our results, 35 proteins associated with Bcl-2 were identified and Hsp90β was confirmed by co-immunoprecipitation and western blot analysis. Protein‑protein interaction (PPI) analysis indicated that Bcl-2‑Hsp90β interactions may be involved in the anti-apoptotic progression of LC. Further results revealed that disruption of the Bcl-2-Hsp90β interaction inhibited the anti-apoptotic ability of Bcl-2 and decreased the caspase activation in LC, which has broad implications for the better understanding of tumor formation, tumor cell survival and development of metastasis due to Bcl-2. Collectively, we report the mechanism by which Bcl-2 functions in LC as an anti-apoptotic factor in relation to its association with proteins and potentially identify a Bcl-2/Hsp90β axis as a novel target for LC therapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jiang LY, Lian M, Wang H, Fang JG and Wang Q: Inhibitory effects of 5-Aza-2-deoxycytidine and trichostatin A in combination with p53-expressing adenovirus on human laryngocarcinoma cells. Chin J Cancer Res. 24:232–237. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Zuo J, Ishikawa T, Boutros S, Xiao Z, Humtsoe JO and Kramer RH: Bcl-2 overexpression induces a partial epithelial to mesenchymal transition and promotes squamous carcinoma cell invasion and metastasis. Mol Cancer Res. 8:170–182. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Kang MH and Reynolds CP: Bcl-2 inhibitors: Targeting mitochondrial apoptotic pathways in cancer therapy. Clin Cancer Res. 15:1126–1132. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Lu S, Yu L, Mu Y, Ma J, Tian J, Xu W and Wang H: Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells. Mol Med Rep. 10:53–60. 2014.PubMed/NCBI

5 

Wu M, Zhang H, Hu J, Weng Z, Li C, Li H, Zhao Y, Mei X, Ren F and Li L: Isoalantolactone inhibits UM-SCC-10A cell growth via cell cycle arrest and apoptosis induction. PLoS One. 8:e760002013. View Article : Google Scholar : PubMed/NCBI

6 

Rahman MA, Amin AR, Wang D, Koenig L, Nannapaneni S, Chen Z, Wang Z, Sica G, Deng X, Chen ZG, et al: RRM2 regulates Bcl-2 in head and neck and lung cancers: A potential target for cancer therapy. Clin Cancer Res. 19:3416–3428. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Kawano K, Kantak SS, Murai M, Yao CC and Kramer RH: Integrin α3β1 engagement disrupts intercellular adhesion. Exp Cell Res. 262:180–196. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Nurmenniemi S, Sinikumpu T, Alahuhta I, Salo S, Sutinen M, Santala M, Risteli J, Nyberg P and Salo T: A novel organotypic model mimics the tumor microenvironment. Am J Pathol. 175:1281–1291. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Zuo JH, Zhu W, Li MY, Li XH, Yi H, Zeng GQ, Wan XX, He QY, Li JH, Qu JQ, et al: Activation of EGFR promotes squamous carcinoma SCC10A cell migration and invasion via inducing EMT-like phenotype change and MMP-9-mediated degradation of E-cadherin. J Cell Biochem. 112:2508–2517. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Cheng AL, Huang WG, Chen ZC, Peng F, Zhang PF, Li MY, Li F, Li JL, Li C, Yi H, et al: Identification of novel nasopharyngeal carcinoma biomarkers by laser capture microdissection and proteomic analysis. Clin Cancer Res. 14:435–445. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Huang WG, Cheng AL, Chen ZC, Peng F, Zhang PF, Li MY, Li F, Li JL, Li C, Yi H, et al: Targeted proteomic analysis of 14-3-3σ in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 42:137–147. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Hu Z, Hung JH, Wang Y, Chang YC, Huang CL, Huyck M and DeLisi C: VisANT 3.5: Multi-scale network visualization, analysis and inference based on the gene ontology. Nucleic Acids Res. 37:(Web Server). W115–W121. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Zuo J, Wen M, Lei M, Peng X, Yang X and Liu Z: MiR-210 links hypoxia with cell proliferation regulation in human Laryngocarcinoma cancer. J Cell Biochem. 116:1039–1049. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Hardwick JM and Soane L: Multiple functions of BCL-2 family proteins. Cold Spring Harb Perspect Biol. 5:a0087222013. View Article : Google Scholar : PubMed/NCBI

15 

Yip KW and Reed JC: Bcl-2 family proteins and cancer. Oncogene. 27:6398–6406. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Yu L and Liu S: Autophagy contributes to modulating the cytotoxicities of Bcl-2 homology domain-3 mimetics. Semin Cancer Biol. 23:553–560. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Marquez RT and Xu L: Bcl-2:Beclin 1 complex: multiple, mechanisms regulating autophagy/apoptosis toggle switch. Am J Cancer Res. 2:214–221. 2012.PubMed/NCBI

18 

Karnak D and Xu L: Chemosensitization of prostate cancer by modulating Bcl-2 family proteins. Curr Drug Targets. 11:699–707. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Buggins AG and Pepper CJ: The role of Bcl-2 family proteins in chronic lymphocytic leukaemia. Leuk Res. 34:837–842. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Mena S, Benlloch M, Ortega A, Carretero J, Obrador E, Asensi M, Petschen I, Brown BD and Estrela JM: Bcl-2 and glutathione depletion sensitizes B16 melanoma to combination therapy and eliminates metastatic disease. Clin Cancer Res. 13:2658–2666. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Xie L, Qin W, Li J, He X, Zhang H, Yao G, Shu H, Yao M, Wan D and Gu J: BNIPL-2 promotes the invasion and metastasis of human hepatocellular carcinoma cells. Oncol Rep. 17:605–610. 2007.PubMed/NCBI

22 

Planas-Silva MD, Bruggeman RD, Grenko RT and Smith JS: Overexpression of c-Myc and Bcl-2 during progression and distant metastasis of hormone-treated breast cancer. Exp Mol Pathol. 82:85–90. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Olsson Åkefeldt S, Ismail MB, Valentin H, Aricò M, Henter JI and Delprat C: Targeting BCL2 family in human myeloid dendritic cells: A challenge to cure diseases with chronic inflammations associated with bone loss. Clin Dev Immunol. 2013:7013052013.PubMed/NCBI

24 

Hu Z, Snitkin ES and DeLisi C: VisANT: An integrative framework for networks in systems biology. Brief Bioinform. 9:317–325. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Hu Z, Chang YC, Wang Y, Huang CL, Liu Y, Tian F, Granger B and Delisi C: VisANT 4.0: Integrative network platform to connect genes, drugs, diseases and therapies. Nucleic Acids Res. 41:(Web Server). W225–W231. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Suderman M and Hallett M: Tools for visually exploring biological networks. Bioinformatics. 23:2651–2659. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Hance MW, Nolan KD and Isaacs JS: The double-edged sword: Conserved functions of extracellular hsp90 in wound healing and cancer. Cancers (Basel). 6:1065–1097. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Tsaytler PA, Krijgsveld J, Goerdayal SS, Rüdiger S and Egmond MR: Novel Hsp90 partners discovered using complementary proteomic approaches. Cell Stress Chaperones. 14:629–638. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Calamia V, de Andrés MC, Oreiro N, Ruiz-Romero C and Blanco FJ: Hsp90b inhibition modulates nitric oxide production and nitric oxide-induced apoptosis in human chondrocytes. BMC Musculoskelet Disord. 12:2372011. View Article : Google Scholar : PubMed/NCBI

30 

Jia W, Yu C, Rahmani M, Krystal G, Sausville EA, Dent P and Grant S: Synergistic antileukemic interactions between 17-AAG and UCN-01 involve interruption of RAF/MEK- and AKT-related pathways. Blood. 102:1824–1832. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Fan Z, Tardif G, Hum D, Duval N, Pelletier JP and Martel-Pelletier J: Hsp90β and p130cas: Novel regulatory factors of MMP-13 expression in human osteoarthritic chondrocytes. Ann Rheum Dis. 68:976–982. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Li J and Buchner J: Structure, function and regulation of the hsp90 machinery. Biomed J. 36:106–117. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Whitesell L and Lin NU: HSP90 as a platform for the assembly of more effective cancer chemotherapy. Biochim Biophys Acta. 1823:756–766. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li S, Li J, Hu T, Zhang C, Lv X, He S, Yan H, Tan Y, Wen M, Lei M, Lei M, et al: Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β. Oncol Rep 37: 849-856, 2017.
APA
Li, S., Li, J., Hu, T., Zhang, C., Lv, X., He, S. ... Zuo, J. (2017). Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β. Oncology Reports, 37, 849-856. https://doi.org/10.3892/or.2016.5295
MLA
Li, S., Li, J., Hu, T., Zhang, C., Lv, X., He, S., Yan, H., Tan, Y., Wen, M., Lei, M., Zuo, J."Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β". Oncology Reports 37.2 (2017): 849-856.
Chicago
Li, S., Li, J., Hu, T., Zhang, C., Lv, X., He, S., Yan, H., Tan, Y., Wen, M., Lei, M., Zuo, J."Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β". Oncology Reports 37, no. 2 (2017): 849-856. https://doi.org/10.3892/or.2016.5295
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Li J, Hu T, Zhang C, Lv X, He S, Yan H, Tan Y, Wen M, Lei M, Lei M, et al: Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β. Oncol Rep 37: 849-856, 2017.
APA
Li, S., Li, J., Hu, T., Zhang, C., Lv, X., He, S. ... Zuo, J. (2017). Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β. Oncology Reports, 37, 849-856. https://doi.org/10.3892/or.2016.5295
MLA
Li, S., Li, J., Hu, T., Zhang, C., Lv, X., He, S., Yan, H., Tan, Y., Wen, M., Lei, M., Zuo, J."Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β". Oncology Reports 37.2 (2017): 849-856.
Chicago
Li, S., Li, J., Hu, T., Zhang, C., Lv, X., He, S., Yan, H., Tan, Y., Wen, M., Lei, M., Zuo, J."Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β". Oncology Reports 37, no. 2 (2017): 849-856. https://doi.org/10.3892/or.2016.5295
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team