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March-2017 Volume 37 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells

  • Authors:
    • Hui Xu
    • Mingping Qian
    • Bingkun Zhao
    • Chenyang Wu
    • Niraj Maskey
    • Hongming Song
    • Dengfeng Li
    • Jialu Song
    • Kaiyao Hua
    • Lin Fang
  • View Affiliations / Copyright

    Affiliations: Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China
  • Pages: 1619-1626
    |
    Published online on: January 25, 2017
       https://doi.org/10.3892/or.2017.5404
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Abstract

RAB1A acts as an oncogene in various cancers, and emerging evidence has verified that RAB1A is an mTORC1 activator in hepatocellular and colorectal cancer, but the role of RAB1A in breast cancer remains unclear. In this investigation, RAB1A siRNA was successfully transfected in MDA-MB-231 and BT-549 human triple-negative breast cancer cells, and verified by real‑time quantitative polymerase chain reaction and western blotting. Then, MTT cell proliferation, colony formation, cell invasion and wound healing assays were performed to characterize the function of RAB1A in the breast cancer cell lines. Downregulation of RAB1A inhibited cellular growth, cell migration, cell invasion and cell epithelial-mesenchymal transition. Furthermore, compared with NC siRNA transfected cells, RAB1A siRNA transfected breast cancer cells inhibited the phosphorylation of S6K1, the effector molecular of mTORC1. Collectively, our data suggested that RAB1A acts as an oncogene by regulating cellular proliferation, growth, invasion and metastasis via activation of mTORC1 pathway in triple-negative breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xu H, Qian M, Zhao B, Wu C, Maskey N, Song H, Li D, Song J, Hua K, Fang L, Fang L, et al: Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells. Oncol Rep 37: 1619-1626, 2017.
APA
Xu, H., Qian, M., Zhao, B., Wu, C., Maskey, N., Song, H. ... Fang, L. (2017). Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells. Oncology Reports, 37, 1619-1626. https://doi.org/10.3892/or.2017.5404
MLA
Xu, H., Qian, M., Zhao, B., Wu, C., Maskey, N., Song, H., Li, D., Song, J., Hua, K., Fang, L."Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells". Oncology Reports 37.3 (2017): 1619-1626.
Chicago
Xu, H., Qian, M., Zhao, B., Wu, C., Maskey, N., Song, H., Li, D., Song, J., Hua, K., Fang, L."Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells". Oncology Reports 37, no. 3 (2017): 1619-1626. https://doi.org/10.3892/or.2017.5404
Copy and paste a formatted citation
x
Spandidos Publications style
Xu H, Qian M, Zhao B, Wu C, Maskey N, Song H, Li D, Song J, Hua K, Fang L, Fang L, et al: Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells. Oncol Rep 37: 1619-1626, 2017.
APA
Xu, H., Qian, M., Zhao, B., Wu, C., Maskey, N., Song, H. ... Fang, L. (2017). Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells. Oncology Reports, 37, 1619-1626. https://doi.org/10.3892/or.2017.5404
MLA
Xu, H., Qian, M., Zhao, B., Wu, C., Maskey, N., Song, H., Li, D., Song, J., Hua, K., Fang, L."Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells". Oncology Reports 37.3 (2017): 1619-1626.
Chicago
Xu, H., Qian, M., Zhao, B., Wu, C., Maskey, N., Song, H., Li, D., Song, J., Hua, K., Fang, L."Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells". Oncology Reports 37, no. 3 (2017): 1619-1626. https://doi.org/10.3892/or.2017.5404
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