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Article

Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin

  • Authors:
    • Zeting Yuan
    • Xiaojing Shi
    • Yanyan Qiu
    • Tingting Jia
    • Xia Yuan
    • Yu Zou
    • Cheng Liu
    • Hui Yu
    • Yuxia Yuan
    • Xue He
    • Ke Xu
    • Peihao Yin
  • View Affiliations / Copyright

    Affiliations: Interventional Cancer Institute of Chinese Integrative Medicine, Putuo Hospital, Shanghai 200062, P.R. China, Department of Pharmacy, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China
  • Pages: 1815-1825
    |
    Published online on: January 30, 2017
       https://doi.org/10.3892/or.2017.5410
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Abstract

Cinobufagin (CBF) is isolated from the skin and posterior auricular glands of the Asiatic toad (Bufo gargarizans). This study investigated the reversal effect of CBF on P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) in colon cancer. The effect of CBF on the cytotoxicity of anticancer drugs in P-gp overexpressing LoVo/ADR, HCT116/L, Cao-2/ADR cells and their parental cells was determined using CCK-8 assay. Apoptosis of anti-cancer drugs and accumulation of doxorubicin (DOX) and Rhodamine 123 (Rho123) in P-gp overexpressing cells were evaluated by flow cytometry. Results indicated that CBF significantly enhanced the sensitivity of P-gp substrate drugs on P-gp overexpressing cells, but had no effect on their parental cells. CBF enhanced the effect of DOX against P-gp-overexpressing LoVo/ADR cell xenografts in nude mice. Moreover, CBF also increased cell apoptosis of chemotherapy agents and intracellular accumulation of DOX and Rho123 in the MDR cells. Further research on the mechanisms revealed non-competitive inhibition of P-gp ATPase activity, but without altering the expression of P-gp. These findings demonstrated that CBF could be further developed into a safe and potent P-gp modulator for combination use with anticancer drugs in cancer chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan Z, Shi X, Qiu Y, Jia T, Yuan X, Zou Y, Liu C, Yu H, Yuan Y, He X, He X, et al: Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin. Oncol Rep 37: 1815-1825, 2017.
APA
Yuan, Z., Shi, X., Qiu, Y., Jia, T., Yuan, X., Zou, Y. ... Yin, P. (2017). Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin. Oncology Reports, 37, 1815-1825. https://doi.org/10.3892/or.2017.5410
MLA
Yuan, Z., Shi, X., Qiu, Y., Jia, T., Yuan, X., Zou, Y., Liu, C., Yu, H., Yuan, Y., He, X., Xu, K., Yin, P."Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin". Oncology Reports 37.3 (2017): 1815-1825.
Chicago
Yuan, Z., Shi, X., Qiu, Y., Jia, T., Yuan, X., Zou, Y., Liu, C., Yu, H., Yuan, Y., He, X., Xu, K., Yin, P."Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin". Oncology Reports 37, no. 3 (2017): 1815-1825. https://doi.org/10.3892/or.2017.5410
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Z, Shi X, Qiu Y, Jia T, Yuan X, Zou Y, Liu C, Yu H, Yuan Y, He X, He X, et al: Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin. Oncol Rep 37: 1815-1825, 2017.
APA
Yuan, Z., Shi, X., Qiu, Y., Jia, T., Yuan, X., Zou, Y. ... Yin, P. (2017). Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin. Oncology Reports, 37, 1815-1825. https://doi.org/10.3892/or.2017.5410
MLA
Yuan, Z., Shi, X., Qiu, Y., Jia, T., Yuan, X., Zou, Y., Liu, C., Yu, H., Yuan, Y., He, X., Xu, K., Yin, P."Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin". Oncology Reports 37.3 (2017): 1815-1825.
Chicago
Yuan, Z., Shi, X., Qiu, Y., Jia, T., Yuan, X., Zou, Y., Liu, C., Yu, H., Yuan, Y., He, X., Xu, K., Yin, P."Reversal of P-gp-mediated multidrug resistance in colon cancer by cinobufagin". Oncology Reports 37, no. 3 (2017): 1815-1825. https://doi.org/10.3892/or.2017.5410
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