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Article

Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation

  • Authors:
    • Dong Liu
    • Rong Zhang
    • Jie Wu
    • Yansong Pu
    • Xiaoran Yin
    • Yan Cheng
    • Jing Wu
    • Cheng Feng
    • Yumei Luo
    • Jun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Gastroenterology, Shaanxi Provincal People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of General Surgery, Shaanxi Provincal People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Oncology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 1779-1785
    |
    Published online on: February 7, 2017
       https://doi.org/10.3892/or.2017.5426
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Abstract

Interleukin-17A (IL-17A), a pro-inflammatory cytokine secreted primarily by Th17 cells, has been proved to be involved in the microenvironment of certain inflammation-related tumors. However, the role of IL-17A in cancer development has always been controversial. In this study, we investigated the effect of IL-17A on the regulation of esophageal adenocarcinoma (EAC) cell invasiveness and related molecular mechanism. Surface IL-17 receptor (IL-17R) expression on human EAC cell line OE19 was examined using flow cytometry. The effect of IL-17A on cell proliferation was measured by MTT assay. Cell migration and invasive ability in vitro were assessed by wound-healing and Matrigel-coated Transwell invasion assay. Intracellular reactive oxygen species (ROS) levels were determined by flow cytometry and fluorescence microscope. The protein expression levels of MMP-2, MMP-9, NF-κB and p-IκB-α were detected by western blotting. Our results showed that IL-17A promoted migration and invasion of OE19 cells in a dose-dependent manner, however it had less effect on OE19 cell proliferation. Furthermore, IL-17A treatment significantly upregulated the expression of MMP-2 and MMP-9, stimulated intracellular ROS production, increased IκB-α phosphorylation and NF-κB nuclear translocation. Nevertheless, IL-17A-induced expression of MMP-2/9 and OE19 cell invasiveness were both inhibited by pretreatment with N-acetyl-L-cysteine (NAC, a ROS scavenger) or pyrrolidine dithiocarbamate (PDTC, a NF-κB inhibitor). In conclusion, these findings demonstrate that IL-17A can promote the migration and invasiveness of EAC cells through ROS/NF-κB/MMP-2/9 signaling pathway activation, indicating that IL-17A may be a potential therapeutic target for EAC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu D, Zhang R, Wu J, Pu Y, Yin X, Cheng Y, Wu J, Feng C, Luo Y, Zhang J, Zhang J, et al: Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation. Oncol Rep 37: 1779-1785, 2017.
APA
Liu, D., Zhang, R., Wu, J., Pu, Y., Yin, X., Cheng, Y. ... Zhang, J. (2017). Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation. Oncology Reports, 37, 1779-1785. https://doi.org/10.3892/or.2017.5426
MLA
Liu, D., Zhang, R., Wu, J., Pu, Y., Yin, X., Cheng, Y., Wu, J., Feng, C., Luo, Y., Zhang, J."Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation". Oncology Reports 37.3 (2017): 1779-1785.
Chicago
Liu, D., Zhang, R., Wu, J., Pu, Y., Yin, X., Cheng, Y., Wu, J., Feng, C., Luo, Y., Zhang, J."Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation". Oncology Reports 37, no. 3 (2017): 1779-1785. https://doi.org/10.3892/or.2017.5426
Copy and paste a formatted citation
x
Spandidos Publications style
Liu D, Zhang R, Wu J, Pu Y, Yin X, Cheng Y, Wu J, Feng C, Luo Y, Zhang J, Zhang J, et al: Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation. Oncol Rep 37: 1779-1785, 2017.
APA
Liu, D., Zhang, R., Wu, J., Pu, Y., Yin, X., Cheng, Y. ... Zhang, J. (2017). Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation. Oncology Reports, 37, 1779-1785. https://doi.org/10.3892/or.2017.5426
MLA
Liu, D., Zhang, R., Wu, J., Pu, Y., Yin, X., Cheng, Y., Wu, J., Feng, C., Luo, Y., Zhang, J."Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation". Oncology Reports 37.3 (2017): 1779-1785.
Chicago
Liu, D., Zhang, R., Wu, J., Pu, Y., Yin, X., Cheng, Y., Wu, J., Feng, C., Luo, Y., Zhang, J."Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation". Oncology Reports 37, no. 3 (2017): 1779-1785. https://doi.org/10.3892/or.2017.5426
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