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Article

MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3

  • Authors:
    • Yonggang Zhu
    • Hongguang Zhao
    • Min Rao
    • Songbai Xu
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Nuclear Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Gastroenterology, The People's Republic Hospital of Jilin Province, Changchun, Jilin 130021, P.R. China, Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 2185-2192
    |
    Published online on: February 16, 2017
       https://doi.org/10.3892/or.2017.5458
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Abstract

A growing body of evidence suggests that microRNA-365 (miR-365) played crucial role in the initiation and development of many types of cancers. However, the biological role of miR-365 in human glioma remains unclear. Herein, the aims of this study were to investigate the role and underlying mechanisms of miR-365 in glioma by a series of in vitro and in vivo experiments. We found that miR-365 was strongly downregulated in malignant glioma tissues and cell lines. Restoration of the expression of miR-365 in glioma cells significantly inhibited cell proliferation, migration and invasion in vitro and tumor growth in vivo. Notably, phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was proved to be a direct target of miR-365 in glioma cells, and its mRNA expression was inversely correlated with miR-365 expression in clinical glioma tissues. PIK3R3 overexpression in miR-365 expressing cells could rescue proliferation, migration and invasion inhibition of miR-365. In addition, miR-365 was able to inhibit the phosphorylation of AKT and mTOR in vitro and in vivo, which are key participants in the AKT/mTOR pathway. These results suggest that miR-365 functioned as a tumor suppressor in glioma by targeting PIK3R3, suggesting that miR-365 has potential as therapeutic targets for glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu Y, Zhao H, Rao M and Xu S: MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3. Oncol Rep 37: 2185-2192, 2017.
APA
Zhu, Y., Zhao, H., Rao, M., & Xu, S. (2017). MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3. Oncology Reports, 37, 2185-2192. https://doi.org/10.3892/or.2017.5458
MLA
Zhu, Y., Zhao, H., Rao, M., Xu, S."MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3". Oncology Reports 37.4 (2017): 2185-2192.
Chicago
Zhu, Y., Zhao, H., Rao, M., Xu, S."MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3". Oncology Reports 37, no. 4 (2017): 2185-2192. https://doi.org/10.3892/or.2017.5458
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu Y, Zhao H, Rao M and Xu S: MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3. Oncol Rep 37: 2185-2192, 2017.
APA
Zhu, Y., Zhao, H., Rao, M., & Xu, S. (2017). MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3. Oncology Reports, 37, 2185-2192. https://doi.org/10.3892/or.2017.5458
MLA
Zhu, Y., Zhao, H., Rao, M., Xu, S."MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3". Oncology Reports 37.4 (2017): 2185-2192.
Chicago
Zhu, Y., Zhao, H., Rao, M., Xu, S."MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3". Oncology Reports 37, no. 4 (2017): 2185-2192. https://doi.org/10.3892/or.2017.5458
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