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Article

Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells

  • Authors:
    • Kyoung-Jin Min
    • Min Ae Han
    • Shin Kim
    • Jong-Wook Park
    • Taeg Kyu Kwon
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Keimyung University, Dalseo-Gu, Daegu 704-701, Republic of Korea
  • Pages: 2348-2354
    |
    Published online on: March 6, 2017
       https://doi.org/10.3892/or.2017.5490
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Abstract

Osthole, an active constituent isolated from the fruit of Cnidium monnieri (L.) Cusson, has been shown to induce various beneficial biochemical effects such as anti-inflammatory and antitumor. In the present study, we examined whether osthole could sensitize TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human renal carcinoma Caki cells. We found that osthole and TRAIL alone, had no effect on apoptosis, but combined treatment with osthole and TRAIL markedly induced apoptosis in Caki (renal carcinoma), U251MG (glioma) and MDA-MB-231 (breast carcinoma) cells. In contrast, combined treatment with osthole and TRAIL did not induce apoptosis in normal human skin fibroblast cells. Osthole induced downregulation of cellular FLICE-like inhibitory protein (c-FLIP) expression, and overexpression of c-FLIP markedly blocked apoptosis induced by the combined treatment with osthole and TRAIL. In addition, osthole markedly reduced mitochondrial membrane potential levels, and increased cytosolic cytochrome c release in combined treatment with osthole and TRAIL. Therefore, these data suggest that osthole may be an efficient TRAIL sensitizer.
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Copy and paste a formatted citation
Spandidos Publications style
Min K, Han MA, Kim S, Park J and Kwon TK: Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells. Oncol Rep 37: 2348-2354, 2017.
APA
Min, K., Han, M.A., Kim, S., Park, J., & Kwon, T.K. (2017). Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells. Oncology Reports, 37, 2348-2354. https://doi.org/10.3892/or.2017.5490
MLA
Min, K., Han, M. A., Kim, S., Park, J., Kwon, T. K."Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells". Oncology Reports 37.4 (2017): 2348-2354.
Chicago
Min, K., Han, M. A., Kim, S., Park, J., Kwon, T. K."Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells". Oncology Reports 37, no. 4 (2017): 2348-2354. https://doi.org/10.3892/or.2017.5490
Copy and paste a formatted citation
x
Spandidos Publications style
Min K, Han MA, Kim S, Park J and Kwon TK: Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells. Oncol Rep 37: 2348-2354, 2017.
APA
Min, K., Han, M.A., Kim, S., Park, J., & Kwon, T.K. (2017). Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells. Oncology Reports, 37, 2348-2354. https://doi.org/10.3892/or.2017.5490
MLA
Min, K., Han, M. A., Kim, S., Park, J., Kwon, T. K."Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells". Oncology Reports 37.4 (2017): 2348-2354.
Chicago
Min, K., Han, M. A., Kim, S., Park, J., Kwon, T. K."Osthole enhances TRAIL-mediated apoptosis through downregulation of c-FLIP expression in renal carcinoma Caki cells". Oncology Reports 37, no. 4 (2017): 2348-2354. https://doi.org/10.3892/or.2017.5490
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