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Article

CIRH1A augments the proliferation of RKO colorectal cancer cells

  • Authors:
    • Feng Guo
    • Jian-Jun Chen
    • Wei-Jun Tang
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, P.R. China
  • Pages: 2375-2381
    |
    Published online on: March 8, 2017
       https://doi.org/10.3892/or.2017.5497
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Abstract

Accumulating evidence suggests that ribosomal proteins may have extraribosomal functions in various physiological and pathological processes, including cancer. We analyzed the expression of the CIRH1A ribosomal protein in colorectal carcinoma and para-carcinoma samples by bioinformatics analyses of data extracted from The Cancer Genome Atlas and in colorectal cancer cell lines in vitro by qPCR. CIRH1A was highly expressed in carcinoma samples and colorectal cancer cells. We also transduced the RKO colorectal cancer (CRC) cell line with lentivirus-mediated small interfering RNAs (siRNAs) and studied the impact that this knockdown of CIRH1A expression had on cell growth. RNA interference (RNAi)-mediated inhibition of CIRH1A expression significantly suppressed proliferation and increased apoptosis of transduced cells, and tended to arrest them in G1 phase. Our data suggest that CIRH1A plays a critical role in the proliferation, cell cycle distribution, and apoptosis of human malignant colorectal cells, and might therefore be a potential target for therapeutic strategies.
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1 

Khan K, Cunningham D and Chau I: Targeting angiogenic pathways in colorectal cancer: Complexities, challenges and future directions. Curr Drug Targets. 18:56–71. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B and Starling N: Colorectal cancer. Lancet. 375:1030–1047. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Qian WF, Guan WX, Gao Y, Tan JF, Qiao ZM, Huang H and Xia CL: Inhibition of STAT3 by RNA interference suppresses angiogenesis in colorectal carcinoma. Braz J Med Biol Res. 44:1222–1230. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Freed EF and Baserga SJ: The C-terminus of Utp4, mutated in childhood cirrhosis, is essential for ribosome biogenesis. Nucleic Acids Res. 38:4798–4806. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Sondalle SB and Baserga SJ: Human diseases of the SSU processome. Biochim Biophys Acta. 1842:758–764. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Doudna JA and Rath VL: Structure and function of the eukaryotic ribosome: The next frontier. Cell. 109:153–156. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Henras AK, Soudet J, Gérus M, Lebaron S, Caizergues-Ferrer M, Mougin A and Henry Y: The post-transcriptional steps of eukaryotic ribosome biogenesis. Cell Mol Life Sci. 65:2334–2359. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Dragon F, Gallagher JE, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, et al: A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 417:967–970. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL and Baserga SJ: RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev. 18:2506–2517. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Phipps KR, Charette J and Baserga SJ: The small subunit processome in ribosome biogenesis - progress and prospects. Wiley Interdiscip Rev RNA. 2:1–21. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Yu B, Mitchell GA and Richter A: Nucleolar localization of cirhin, the protein mutated in North American Indian childhood cirrhosis. Exp Cell Res. 311:218–228. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Chagnon P, Michaud J, Mitchell G, Mercier J, Marion JF, Drouin E, Rasquin-Weber A, Hudson TJ and Richter A: A missense mutation (R565W) in cirhin (FLJ14728) in North American Indian childhood cirrhosis. Am J Hum Genet. 71:1443–1449. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Drouin E, Russo P, Tuchweber B, Mitchell G and Rasquin-Weber A: North American Indian cirrhosis in children: A review of 30 cases. J Pediatr Gastroenterol Nutr. 31:395–404. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Wilkins BJ, Lorent K, Matthews RP and Pack M: p53-mediated biliary defects caused by knockdown of cirh1a, the zebrafish homolog of the gene responsible for North American Indian Childhood Cirrhosis. PLoS One. 8:e776702013. View Article : Google Scholar : PubMed/NCBI

17 

Muzny DM, Bainbridge MN, Chang K, Dinh HH, Drummond JA, Fowler G, Kovar CL, Lewis LR, Morgan MB, Newsham IF, et al: Cancer Genome Atlas Network: Comprehensive molecular characterization of human colon and rectal cancer. Nature. 487:330–337. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Robinson MD and Oshlack A: A scaling normalization method for differential expression analysis of RNA-seq data. Genome Biol. 11:R252010. View Article : Google Scholar : PubMed/NCBI

19 

Robinson MD, McCarthy DJ and Smyth GK: edgeR: A Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 26:139–140. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Yu D, Huber W and Vitek O: Shrinkage estimation of dispersion in Negative Binomial models for RNA-seq experiments with small sample size. Bioinformatics. 29:1275–1282. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Robinson MD and Smyth GK: Small-sample estimation of negative binomial dispersion, with applications to SAGE data. Biostatistics. 9:321–332. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Lund SP, Nettleton D, McCarthy DJ and Smyth GK: Detecting differential expression in RNA-sequence data using quasi-likelihood with shrunken dispersion estimates. Stat Appl Genet Mol Biol. 11:307–314. 2012.

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Lois C, Hong EJ, Pease S, Brown EJ and Baltimore D: Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science. 295:868–872. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

26 

Zhou Y, Su Z, Huang Y, Sun T, Chen S, Wu T, Chen G, Xie X, Li B and Du Z: The Zfx gene is expressed in human gliomas and is important in the proliferation and apoptosis of the human malignant glioma cell line U251. J Exp Clin Cancer Res. 30:1142011. View Article : Google Scholar : PubMed/NCBI

27 

Milner AE, Levens JM and Gregory CD: Flow cytometric methods of analyzing apoptotic cells. Methods Mol Biol. 80:347–354. 1998. View Article : Google Scholar : PubMed/NCBI

28 

Bétard C, Rasquin-Weber A, Brewer C, Drouin E, Clark S, Verner A, Darmond-Zwaig C, Fortin J, Mercier J, Chagnon P, et al: Localization of a recessive gene for North American Indian childhood cirrhosis to chromosome region 16q22-and identification of a shared haplotype. Am J Hum Genet. 67:222–228. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Prieto JL and McStay B: Recruitment of factors linking transcription and processing of pre-rRNA to NOR chromatin is UBF-dependent and occurs independent of transcription in human cells. Genes Dev. 21:2041–2054. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Richter A, Mitchell GA and Rasquin A: North American Indian childhood cirrhosis (NAIC). Med Sci (Paris). 23:1002–1007. 2007.(In French). View Article : Google Scholar : PubMed/NCBI

31 

Yu B, Mitchell GA and Richter A: Cirhin up-regulates a canonical NF-kappaB element through strong interaction with Cirip/HIVEP1. Exp Cell Res. 315:3086–3098. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Freed EF, Prieto JL, McCann KL, McStay B and Baserga SJ: NOL11, implicated in the pathogenesis of North American Indian childhood cirrhosis, is required for pre-rRNA transcription and processing. PLoS Genet. 8:e10028922012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guo F, Chen J and Tang W: CIRH1A augments the proliferation of RKO colorectal cancer cells. Oncol Rep 37: 2375-2381, 2017.
APA
Guo, F., Chen, J., & Tang, W. (2017). CIRH1A augments the proliferation of RKO colorectal cancer cells. Oncology Reports, 37, 2375-2381. https://doi.org/10.3892/or.2017.5497
MLA
Guo, F., Chen, J., Tang, W."CIRH1A augments the proliferation of RKO colorectal cancer cells". Oncology Reports 37.4 (2017): 2375-2381.
Chicago
Guo, F., Chen, J., Tang, W."CIRH1A augments the proliferation of RKO colorectal cancer cells". Oncology Reports 37, no. 4 (2017): 2375-2381. https://doi.org/10.3892/or.2017.5497
Copy and paste a formatted citation
x
Spandidos Publications style
Guo F, Chen J and Tang W: CIRH1A augments the proliferation of RKO colorectal cancer cells. Oncol Rep 37: 2375-2381, 2017.
APA
Guo, F., Chen, J., & Tang, W. (2017). CIRH1A augments the proliferation of RKO colorectal cancer cells. Oncology Reports, 37, 2375-2381. https://doi.org/10.3892/or.2017.5497
MLA
Guo, F., Chen, J., Tang, W."CIRH1A augments the proliferation of RKO colorectal cancer cells". Oncology Reports 37.4 (2017): 2375-2381.
Chicago
Guo, F., Chen, J., Tang, W."CIRH1A augments the proliferation of RKO colorectal cancer cells". Oncology Reports 37, no. 4 (2017): 2375-2381. https://doi.org/10.3892/or.2017.5497
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