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Article

BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway

  • Authors:
    • Xingchun Wang
    • Minjie Li
    • Mengyao Hu
    • Ping Wei
    • Wei Zhu
  • View Affiliations / Copyright

    Affiliations: Department of The First Internal Medicine, The Cancer Hospital of Linyi, Linyi, Shandong 276001, P.R. China, Department of The Third Internal Medicine, The Cancer Hospital of Linyi, Linyi, Shandong 276001, P.R. China
  • Pages: 3046-3054
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    Published online on: March 15, 2017
       https://doi.org/10.3892/or.2017.5508
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Abstract

Non-small cell lung cancer (NSCLC) has the highest mortality rate among all solid tumors with a poor prognosis. The BMP and activin receptor membrane bound inhibitor (BAMBI) has been identified as a hallmark of NSCLC and β-sitosterol possesses antitumor potentiality. This study explores the effect of BAMBI overexpression and β-sitosterol in the context of NSCLC. The results revealed that the transfection of pcDNA‑BAMBI and β-sitosterol treatment significantly reduced the levels of autophagy markers light chain 3 (LC3) II and Beclin 1, whereas the levels of LC3 I and p62 were promoted. The reduced punctate accumulations of GFP-LC3 were detected in pcDNA-BAMBI and β-sitosterol groups, especially in pcDNA-BAMBI + β-sitosterol group. BAMBI overexpression and β-sitosterol induced G0/G1 cell cycle arrest and inhibted cell proliferation in A549 cells. In addition, the levels of transforming growth factor-β (TGF‑β)/p-Smad2/3/c-Myc pathway proteins were decreased. The TGF-β overexpression further confirmed that BAMBI overexpression and β-sitosterol treatment suppre­ssed autohagy and viability of A549 cells was through TGF-β/Smad2/3/c-Myc pathway. Finally, the tumor growth was suppressed in NSCLC xenografts, and the inhibitory effect was stronger under treatment of pcDNA-BAMBI toge­ther with β-sitosterol. These results indicate that BAMBI overexpression and β-sitosterol may serve as novel targets for the treatment of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Li M, Hu M, Wei P and Zhu W: BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway. Oncol Rep 37: 3046-3054, 2017.
APA
Wang, X., Li, M., Hu, M., Wei, P., & Zhu, W. (2017). BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway. Oncology Reports, 37, 3046-3054. https://doi.org/10.3892/or.2017.5508
MLA
Wang, X., Li, M., Hu, M., Wei, P., Zhu, W."BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway". Oncology Reports 37.5 (2017): 3046-3054.
Chicago
Wang, X., Li, M., Hu, M., Wei, P., Zhu, W."BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway". Oncology Reports 37, no. 5 (2017): 3046-3054. https://doi.org/10.3892/or.2017.5508
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Li M, Hu M, Wei P and Zhu W: BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway. Oncol Rep 37: 3046-3054, 2017.
APA
Wang, X., Li, M., Hu, M., Wei, P., & Zhu, W. (2017). BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway. Oncology Reports, 37, 3046-3054. https://doi.org/10.3892/or.2017.5508
MLA
Wang, X., Li, M., Hu, M., Wei, P., Zhu, W."BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway". Oncology Reports 37.5 (2017): 3046-3054.
Chicago
Wang, X., Li, M., Hu, M., Wei, P., Zhu, W."BAMBI overexpression together with β-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-β/Smad2/3 pathway". Oncology Reports 37, no. 5 (2017): 3046-3054. https://doi.org/10.3892/or.2017.5508
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