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Article

Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells

  • Authors:
    • Zhengguang Wang
    • Mingsi Deng
    • Zhendong Liu
    • Song Wu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Orthodontics, The Stomatological Hospital of Changsha, Changsha, Hunan 410005, P.R. China
  • Pages: 3010-3018
    |
    Published online on: March 16, 2017
       https://doi.org/10.3892/or.2017.5511
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Abstract

Hypoxia, a dominant feature in cancer occurrence and evolution, exists throughout the progression of most malignant tumors. This study focused on the mechanism of hypoxia-induced miR-210 upregulation, and the miR-210 functions in schwannoma. We detected microvascular density, vascular endothelial growth factor (VEGF) and miR-210 expression levels using schwannoma tissue mciroarray. The results showed that miR-210 expression was significantly associated with VEGF. Moreover, the cytological tests showed that hypoxia induced miR-210 expression, while reduce ephrin-A3 expression. The bisulfate genomic sequencing PCR results showed that miR-210 promoter region was hypermethylated in RT4-D6P2T in normoxia, while demethylated in hypoxia, and the region included the hypoxia-inducible factor-1α (HIF-1α) response element site. Cellular function research showed that hypoxia resulted in RT4-D6P2T apoptosis, higher autophage and invasion. Besides, hypoxia can affect HIF-1α/VEGF-mediated angiogenesis. To learn about the specific functions of miR-210, we found that with miR-210 inhibition, tumor cell apoptosis increased, autophagy and angiogenesis reduced, and the cell cycle was arrested. Hypoxia promoted miR-210 expression through promoter demethylation, then consequently enhanced tumor cell proliferation and autophagy, increasing tumor cell angiogenesis. Thus, miR-210 could be a potential marker for judging tumor malignancy and be taken as an effective target for clinical auxiliary treatment of neurilemmoma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Deng M, Liu Z and Wu S: Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells. Oncol Rep 37: 3010-3018, 2017.
APA
Wang, Z., Deng, M., Liu, Z., & Wu, S. (2017). Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells. Oncology Reports, 37, 3010-3018. https://doi.org/10.3892/or.2017.5511
MLA
Wang, Z., Deng, M., Liu, Z., Wu, S."Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells". Oncology Reports 37.5 (2017): 3010-3018.
Chicago
Wang, Z., Deng, M., Liu, Z., Wu, S."Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells". Oncology Reports 37, no. 5 (2017): 3010-3018. https://doi.org/10.3892/or.2017.5511
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Deng M, Liu Z and Wu S: Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells. Oncol Rep 37: 3010-3018, 2017.
APA
Wang, Z., Deng, M., Liu, Z., & Wu, S. (2017). Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells. Oncology Reports, 37, 3010-3018. https://doi.org/10.3892/or.2017.5511
MLA
Wang, Z., Deng, M., Liu, Z., Wu, S."Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells". Oncology Reports 37.5 (2017): 3010-3018.
Chicago
Wang, Z., Deng, M., Liu, Z., Wu, S."Hypoxia-induced miR-210 promoter demethylation enhances proliferation, autophagy and angiogenesis of schwannoma cells". Oncology Reports 37, no. 5 (2017): 3010-3018. https://doi.org/10.3892/or.2017.5511
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