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Article

ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma

  • Authors:
    • Ming-Ke Qiu
    • Shu-Qing Wang
    • Chang Pan
    • Yang Wang
    • Zhi-Wei Quan
    • Ying-Bin Liu
    • Jing-Min Ou
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China
  • Pages: 2987-2993
    |
    Published online on: March 21, 2017
       https://doi.org/10.3892/or.2017.5515
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Abstract

Gene expression was examined in hemangiomas (HA), benign, birthmark-like tumors occurring in infancy, and confirmed in HA-derived endothelial cells (HDEC), for which cell proliferation and apoptosis were also assessed. Protein and mRNA accumulation of Rho-associated protein kinase (ROCK), vascular endothelial growth factor (VEGF), Ki-67 and proliferating cell nuclear antigen was significantly higher in proliferating phase HAs than in involuting phase HAs. In contrast, p53 and caspase-3 exhibited higher levels of accumulation in involuting than proliferating HAs. Cell apoptotic indexes were low in proliferating phase HAs and increased in involuting phase HAs. HDECs were treated with the ROCK inhibitor Y-27632. Y-27632 induced p53 expression and downregulated VEGF expression, significantly inhibited cell proliferation, and induced cell apoptosis in HA cells. The inhibitor effects were confirmed in HAs from HDEC-injected nude mice. These results indicated that ROCK is involved in p53-mediated apoptosis and VEGF expression in HA cells and suggested that such inhibition may be exploited for future HA therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu M, Wang S, Pan C, Wang Y, Quan Z, Liu Y and Ou J: ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma. Oncol Rep 37: 2987-2993, 2017.
APA
Qiu, M., Wang, S., Pan, C., Wang, Y., Quan, Z., Liu, Y., & Ou, J. (2017). ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma. Oncology Reports, 37, 2987-2993. https://doi.org/10.3892/or.2017.5515
MLA
Qiu, M., Wang, S., Pan, C., Wang, Y., Quan, Z., Liu, Y., Ou, J."ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma". Oncology Reports 37.5 (2017): 2987-2993.
Chicago
Qiu, M., Wang, S., Pan, C., Wang, Y., Quan, Z., Liu, Y., Ou, J."ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma". Oncology Reports 37, no. 5 (2017): 2987-2993. https://doi.org/10.3892/or.2017.5515
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu M, Wang S, Pan C, Wang Y, Quan Z, Liu Y and Ou J: ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma. Oncol Rep 37: 2987-2993, 2017.
APA
Qiu, M., Wang, S., Pan, C., Wang, Y., Quan, Z., Liu, Y., & Ou, J. (2017). ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma. Oncology Reports, 37, 2987-2993. https://doi.org/10.3892/or.2017.5515
MLA
Qiu, M., Wang, S., Pan, C., Wang, Y., Quan, Z., Liu, Y., Ou, J."ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma". Oncology Reports 37.5 (2017): 2987-2993.
Chicago
Qiu, M., Wang, S., Pan, C., Wang, Y., Quan, Z., Liu, Y., Ou, J."ROCK inhibition as a potential therapeutic target involved in apoptosis in hemangioma". Oncology Reports 37, no. 5 (2017): 2987-2993. https://doi.org/10.3892/or.2017.5515
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