Open Access

The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer

  • Authors:
    • Gang Chen
    • Hefen Yu
    • Lucy Satherley
    • Catherine Zabkiewicz
    • Jeyna Resaul
    • Huishan Zhao
    • Hu Mu
    • Xiuyi Zhi
    • Junqi He
    • Lin Ye
    • Wen G. Jiang
  • View Affiliations

  • Published online on: March 31, 2017     https://doi.org/10.3892/or.2017.5538
  • Pages: 2695-2701
  • Copyright : © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

The downstream of tyrosine kinase 7 (DOK7) is an adaptor protein mediating signalling transduction between receptors and intracellular downstream molecules. Reduced expression of DOK7 has been observed in breast cancer. The present study aimed to investigate the role played by DOK7 in lung cancer. The expression of DOK7 at both mRNA and protein levels was evaluated in human lung cancer. A reduced expression of DOK7 transcripts was seen in lung cancers compared with normal lung tissues. Kaplan-Meier analyses showed that the reduced expression of DOK7 was associated with poorer overall survival and progression-free survival of patients with lung cancer. A further western blot analysis revealed a predominant expression of DOK7 isoform 1 (DOK7V1) in normal lung tissues, which was reduced in lung cancer. Forced overexpression of DOK7V1 in lung cancer cell lines, A549 and H3122 resulted in a decrease of in vitro cell proliferation and migration, while adhesion to extracellular matrix was enhanced following the expression. In conclusion, DOK7 was reduced in lung cancer and reduced DOK7 expression was associated with poorer survival. DOK7 isoform 1 plays an inhibitory role on the proliferation and migration of lung cancer cells in which Akt pathway may be involved.
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May-2017
Volume 37 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen G, Yu H, Satherley L, Zabkiewicz C, Resaul J, Zhao H, Mu H, Zhi X, He J, Ye L, Ye L, et al: The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer. Oncol Rep 37: 2695-2701, 2017.
APA
Chen, G., Yu, H., Satherley, L., Zabkiewicz, C., Resaul, J., Zhao, H. ... Jiang, W.G. (2017). The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer. Oncology Reports, 37, 2695-2701. https://doi.org/10.3892/or.2017.5538
MLA
Chen, G., Yu, H., Satherley, L., Zabkiewicz, C., Resaul, J., Zhao, H., Mu, H., Zhi, X., He, J., Ye, L., Jiang, W. G."The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer". Oncology Reports 37.5 (2017): 2695-2701.
Chicago
Chen, G., Yu, H., Satherley, L., Zabkiewicz, C., Resaul, J., Zhao, H., Mu, H., Zhi, X., He, J., Ye, L., Jiang, W. G."The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer". Oncology Reports 37, no. 5 (2017): 2695-2701. https://doi.org/10.3892/or.2017.5538