miR-107 functions as a tumor suppressor in human esophageal squamous cell carcinoma and targets Cdc42

  • Authors:
    • Priyanka Sharma
    • Neeru Saini
    • Rinu Sharma
  • View Affiliations

  • Published online on: April 3, 2017     https://doi.org/10.3892/or.2017.5546
  • Pages: 3116-3127
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Abstract

Previously, we reported significantly decreased expression of tissue and circulating miR-107 in esophageal cancer (EC). However, its role in esophageal tumorigenesis still remains elusive. Therefore, the aim of the present study was to analyze the role of miR-107 in esophageal squamous cell carcinoma (ESCC). The role of miR-107 in ESCC was evaluated using MTT assay, cell cycle analysis by flow cytometry, annexin assay, colony formation assay and scratch assay. Overexpression of miR-107 in KYSE-410 cells suppressed cell proliferation at 72 h post-transfection (p=0.0001). Moreover, a significant increase in the G0/G1 population (p<0.001) and a significant decrease in the G2/M (p=0.032) population was also observed in the miR-107-treated cells as compared to the negative control (NC). Notably, miR-107 overexpression attenuated the colony formation potential of ESCC cells by 41.83% as compared to the NC (p=0.007). miR-107 mimic inhibited ESCC cell migration in a time-dependent manner, reducing the wound closure to only 50.41±7.23% at 72 h post-transfection (p=0.041). Further analysis by Matrigel invasion assay revealed a significant decrease in the migratory and invasive abilities of the KYSE-410 cells at 72 h post miR-107 transfection. qRT-PCR analysis showed decreased expression of one of the newly identified targets of miR-107, Cdc42, at the mRNA level. Further validation by western blotting confirmed a significant reduction in the identified target at the protein level. In addition, the relative luciferase activity of the reporter containing Cdc42 3'UTR was significantly decreased upon miR-107 co-transfection, indicating it to be a direct target of miR-107. Our results herein document that miR-107 functions as a tumor suppressor and inhibits the proliferation, migration and invasion of ESCC cells. Moreover, this is the first report showing Cdc42 as a downstream target of miR-107.
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May-2017
Volume 37 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sharma P, Saini N and Sharma R: miR-107 functions as a tumor suppressor in human esophageal squamous cell carcinoma and targets Cdc42 . Oncol Rep 37: 3116-3127, 2017
APA
Sharma, P., Saini, N., & Sharma, R. (2017). miR-107 functions as a tumor suppressor in human esophageal squamous cell carcinoma and targets Cdc42 . Oncology Reports, 37, 3116-3127. https://doi.org/10.3892/or.2017.5546
MLA
Sharma, P., Saini, N., Sharma, R."miR-107 functions as a tumor suppressor in human esophageal squamous cell carcinoma and targets Cdc42 ". Oncology Reports 37.5 (2017): 3116-3127.
Chicago
Sharma, P., Saini, N., Sharma, R."miR-107 functions as a tumor suppressor in human esophageal squamous cell carcinoma and targets Cdc42 ". Oncology Reports 37, no. 5 (2017): 3116-3127. https://doi.org/10.3892/or.2017.5546