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Article Open Access

miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2

  • Authors:
    • Jiangtu He
    • Jieying Mai
    • Yan Li
    • Linyi Chen
    • Hui Xu
    • Xiaofei Zhu
    • Qiuhui Pan
  • View Affiliations / Copyright

    Affiliations: The Research Center for Immunology, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Central Laboratory, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China, Department of Clinical Laboratory, Maternal and Child Health Care of Zaozhuang, Zaozhuang, Shandong 277102, P.R. China, Department of Ophthalmology, Tai Zhou Hospital of Zhejiang Province, Taizhou, Zhejiang 317099, P.R China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2672-2678
    |
    Published online on: April 5, 2017
       https://doi.org/10.3892/or.2017.5558
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Abstract

Many reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutical target for many diseases, even of human cancers; however, there are no reports on the role of miR-597 in human cancers. In the present study, by detecting mRNA expression with qRT-PCR, compared with the adjacent normal tissues we found that miR-597 was significantly downregulated in breast cancer tissues. By using the MTT assay, the cell wound-healing assay and the cell invasion assay, we demonstrated that miR-597 mimics were able to suppress breast cancer cell proliferation, migration and invasion. Additionally, with flow cytometry, we found that mir-597 influenced the growth of breast cancer cells through regulating the G1-S phase transition. Furthermore, we identified one binding site for miR-597 at the 3'UTR of the FOSL2 gene, using bioinformatics methods and the luciferase reporter assay, it was confirmed that FOSL2 was a direct target of miR-597. Moreover, overexpression of FOSL2 in MDA-MB‑231 and SK-BR-3 cells can block the vast majority of the miR-597 roles, suggesting that miR-597 acts as a tumor suppressor in breast cancer cells by the downregulation of FOSL2. Additionally, we also found a negative correlation between the expression of FOSL2 and miR-597 in the tumor samples. This new regulatory mechanism in breast cancer may provide another method for diagnosis and therapy.
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Copy and paste a formatted citation
Spandidos Publications style
He J, Mai J, Li Y, Chen L, Xu H, Zhu X and Pan Q: miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncol Rep 37: 2672-2678, 2017.
APA
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., & Pan, Q. (2017). miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncology Reports, 37, 2672-2678. https://doi.org/10.3892/or.2017.5558
MLA
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., Pan, Q."miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2". Oncology Reports 37.5 (2017): 2672-2678.
Chicago
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., Pan, Q."miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2". Oncology Reports 37, no. 5 (2017): 2672-2678. https://doi.org/10.3892/or.2017.5558
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Mai J, Li Y, Chen L, Xu H, Zhu X and Pan Q: miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncol Rep 37: 2672-2678, 2017.
APA
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., & Pan, Q. (2017). miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncology Reports, 37, 2672-2678. https://doi.org/10.3892/or.2017.5558
MLA
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., Pan, Q."miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2". Oncology Reports 37.5 (2017): 2672-2678.
Chicago
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., Pan, Q."miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2". Oncology Reports 37, no. 5 (2017): 2672-2678. https://doi.org/10.3892/or.2017.5558
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