Open Access

miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2

  • Authors:
    • Jiangtu He
    • Jieying Mai
    • Yan Li
    • Linyi Chen
    • Hui Xu
    • Xiaofei Zhu
    • Qiuhui Pan
  • View Affiliations

  • Published online on: April 5, 2017     https://doi.org/10.3892/or.2017.5558
  • Pages: 2672-2678
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Many reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutical target for many diseases, even of human cancers; however, there are no reports on the role of miR-597 in human cancers. In the present study, by detecting mRNA expression with qRT-PCR, compared with the adjacent normal tissues we found that miR-597 was significantly downregulated in breast cancer tissues. By using the MTT assay, the cell wound-healing assay and the cell invasion assay, we demonstrated that miR-597 mimics were able to suppress breast cancer cell proliferation, migration and invasion. Additionally, with flow cytometry, we found that mir-597 influenced the growth of breast cancer cells through regulating the G1-S phase transition. Furthermore, we identified one binding site for miR-597 at the 3'UTR of the FOSL2 gene, using bioinformatics methods and the luciferase reporter assay, it was confirmed that FOSL2 was a direct target of miR-597. Moreover, overexpression of FOSL2 in MDA-MB‑231 and SK-BR-3 cells can block the vast majority of the miR-597 roles, suggesting that miR-597 acts as a tumor suppressor in breast cancer cells by the downregulation of FOSL2. Additionally, we also found a negative correlation between the expression of FOSL2 and miR-597 in the tumor samples. This new regulatory mechanism in breast cancer may provide another method for diagnosis and therapy.
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May-2017
Volume 37 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
He J, Mai J, Li Y, Chen L, Xu H, Zhu X and Pan Q: miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncol Rep 37: 2672-2678, 2017
APA
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., & Pan, Q. (2017). miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncology Reports, 37, 2672-2678. https://doi.org/10.3892/or.2017.5558
MLA
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., Pan, Q."miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2". Oncology Reports 37.5 (2017): 2672-2678.
Chicago
He, J., Mai, J., Li, Y., Chen, L., Xu, H., Zhu, X., Pan, Q."miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2". Oncology Reports 37, no. 5 (2017): 2672-2678. https://doi.org/10.3892/or.2017.5558