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Article

Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma

  • Authors:
    • Mitsuyoshi Ohshima
    • Keisuke Sugahara
    • Kiyohiro Kasahara
    • Akira Katakura
  • View Affiliations / Copyright

    Affiliations: Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, Chiyoda, Tokyo 101-0061, Japan
  • Pages: 2727-2734
    |
    Published online on: April 6, 2017
       https://doi.org/10.3892/or.2017.5561
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Abstract

The aim of the present study was to characterize the metabolic systems in Japanese patients with oral squamous cell carcinoma (OSCC) using capillary electrophoresis-mass spectrometry (CE-MS) metabolome analysis of saliva samples. A previous study showed variations among ethnicities and tumor sites in the saliva metabolome of patients with OSCC using CE-MS. In the present study, saliva was obtained from 22 Japanese patients with OSCC and from 21 healthy controls who visited the Department of Dentistry, Oral and Maxillofacial Surgery, Tokyo Dental Collage Ichikawa General Hospital, Tokyo, Japan, and all samples were subject to comprehensive quantitative metabolome analysis using CE-MS. A total of 499 metabolites were detected as CE-MS peaks in the saliva tested from the two groups. A total of 25 metabolites were revealed as potential markers to discriminate between patients with OSCC and healthy controls: Choline, p-hydroxyphenylacetic acid, and 2-hydroxy-4-methylvaleric acid (P<0.001); valine, 3-phenyllactic acid, leucine, hexanoic acid, octanoic acid, terephthalic acid, γ-butyrobetaine, and 3-(4-hydroxyphenyl)propionic acid (P<0.01); and isoleucine, tryptophan, 3-phenylpropionic acid, 2-hydroxyvaleric acid, butyric acid, cadaverine, 2-oxoisovaleric acid, N6,N6,N6-trimethyllysine, taurine, glycolic acid, 3-hydroxybutyric acid, heptanoic acid, alanine, and urea (P<0.05, according to the Wilcoxon rank sum test). A previous study by Sugimoto and co-workers detected 24 discriminatory metabolites, 7 of which (taurine, valine, leucine, isoleucine, choline, cadaverine, and tryptophan) were also detected in the present study. In the present study, however, choline, metabolites in the branched chain amino acids (BCAA) cycle, urea, and 3-hydroxybutyric acid were also characterized. Choline and metabolites of the BCAA cycle have previously been reported in OSCC using metabolome analysis. To the best of our knowledge, no previous reports have identified urea and 3-hydroxybuyric acid in the metabolome of patients with OSCC. These findings suggest the usefulness of metabolites as salivary biomarkers for Japanese patients with OSCC. Further studies using larger patient cohorts should be conducted to validate these results.
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Copy and paste a formatted citation
Spandidos Publications style
Ohshima M, Sugahara K, Kasahara K and Katakura A: Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma. Oncol Rep 37: 2727-2734, 2017.
APA
Ohshima, M., Sugahara, K., Kasahara, K., & Katakura, A. (2017). Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma. Oncology Reports, 37, 2727-2734. https://doi.org/10.3892/or.2017.5561
MLA
Ohshima, M., Sugahara, K., Kasahara, K., Katakura, A."Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma". Oncology Reports 37.5 (2017): 2727-2734.
Chicago
Ohshima, M., Sugahara, K., Kasahara, K., Katakura, A."Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma". Oncology Reports 37, no. 5 (2017): 2727-2734. https://doi.org/10.3892/or.2017.5561
Copy and paste a formatted citation
x
Spandidos Publications style
Ohshima M, Sugahara K, Kasahara K and Katakura A: Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma. Oncol Rep 37: 2727-2734, 2017.
APA
Ohshima, M., Sugahara, K., Kasahara, K., & Katakura, A. (2017). Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma. Oncology Reports, 37, 2727-2734. https://doi.org/10.3892/or.2017.5561
MLA
Ohshima, M., Sugahara, K., Kasahara, K., Katakura, A."Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma". Oncology Reports 37.5 (2017): 2727-2734.
Chicago
Ohshima, M., Sugahara, K., Kasahara, K., Katakura, A."Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma". Oncology Reports 37, no. 5 (2017): 2727-2734. https://doi.org/10.3892/or.2017.5561
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