Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
May-2017 Volume 37 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2017 Volume 37 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells

  • Authors:
    • Hong Zhu
    • Qing Mao
    • Weiwei Liu
    • Zhenhai Yang
    • Xiaoqing Jian
    • Le Qu
    • Chundi He
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Dermatology, Chengdu Fifth People's Hospital, Chengdu, Sichuan 611130, P.R. China, Department of Dermatology, Chaoyang Second Hospital, Chaoyang, Liaoning 122000, P.R. China
  • Pages: 2875-2882
    |
    Published online on: April 11, 2017
       https://doi.org/10.3892/or.2017.5574
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cutaneous squamous cell carcinoma (cSCC) is a common malignant tumor. Mammary serine protease inhibitor (Maspin), a member of serpin family, has been reported as a tumor suppressor in various carcinomas. In this study, we detected the expression level of Maspin in cSCC tissues by real-time PCR and western blotting, and found that Maspin was downregulated in the cSCC tissues compared with the adjacent normal tissues. Moreover, Maspin was stably overexpressed in A431 cells, and CCK-8 assay, colony formation assay, Transwell assay, Hoechst staining and western blotting were carried out to detect the growth, proliferation, invasion, cell cycle and apoptosis of A431 cells. The results revealed that overexpression of Maspin inhibited growth, proliferation, invasion and cell cycle G1/S/G2 transition and enhanced apoptosis of A431 cells. The pro-apoptotic protein cleaved caspase-3, poly(ADP-ribose) polymerase (PARP) and Bax increased, and the anti-apoptotic protein Bcl-2 decreased after Maspin overexpression. Therefore, we demonstrated that Maspin suppressed growth, proliferation and invasion by delaying cell cycle transition and promoting apoptosis in cSCC cells, which may provide new insights for the clinical diagnosis and therapy of cSCC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Lohmann CM and Solomon AR: Clinicopathologic variants of cutaneous squamous cell carcinoma. Adv Anat Pathol. 8:27–36. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Alam M and Ratner D: Cutaneous squamous-cell carcinoma. N Engl J Med. 344:975–983. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Bonerandi JJ, Beauvillain C, Caquant L, Chassagne JF, Chaussade V, Clavère P, Desouches C, Garnier F, Grolleau JL, Grossin M, et al: French Dermatology Recommendations Association (aRED): Guidelines for the diagnosis and treatment of cutaneous squamous cell carcinoma and precursor lesions. J Eur Acad Dermatol Venereol. 25:(Suppl 5). 1–51. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Aboutalebi S and Strickland FM: Immune protection, natural products, and skin cancer: Is there anything new under the sun? J Drugs Dermatol. 5:512–517. 2006.PubMed/NCBI

5 

Weinberg AS, Ogle CA and Shim EK: Metastatic cutaneous squamous cell carcinoma: An update. Dermatol Surg. 33:885–899. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Lippman SM, Parkinson DR, Itri LM, Weber RS, Schantz SP, Ota DM, Schusterman MA, Krakoff IH, Gutterman JU and Hong WK: 13-cis-retinoic acid and interferon alpha-2a: Effective combination therapy for advanced squamous cell carcinoma of the skin. J Natl Cancer Inst. 84:235–241. 1992. View Article : Google Scholar : PubMed/NCBI

7 

Kwa RE, Campana K and Moy RL: Biology of cutaneous squamous cell carcinoma. J Am Acad Dermatol. 26:1–26. 1992. View Article : Google Scholar : PubMed/NCBI

8 

Tanvetyanon T, Padhya T, McCaffrey J, Kish JA, Deconti RC, Trotti A and Rao NG: Postoperative concurrent chemotherapy and radiotherapy for high-risk cutaneous squamous cell carcinoma of the head and neck. Head Neck. 37:840–845. 2015. View Article : Google Scholar : PubMed/NCBI

9 

El-Maqsoud Abd NM and Tawfiek ER: Loss of Maspin expression in bladder cancer: Its relationship with p53 and clinicopathological parameters. J Egypt Natl Canc Inst. 22:1–12. 2010.PubMed/NCBI

10 

Hopkins PC and Whisstock J: Function of maspin. Science. 265:1893–1894. 1994. View Article : Google Scholar : PubMed/NCBI

11 

Pemberton PA, Wong DT, Gibson HL, Kiefer MC, Fitzpatrick PA, Sager R and Barr PJ: The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases. Evidence that maspin is not a protease inhibitory serpin. J Biol Chem. 270:15832–15837. 1995. View Article : Google Scholar : PubMed/NCBI

12 

Al-Ayyoubi M, Gettins PG and Volz K: Crystal structure of human maspin, a serpin with antitumor properties: Reactive center loop of maspin is exposed but constrained. J Biol Chem. 279:55540–55544. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Bodenstine TM, Seftor RE, Khalkhali-Ellis Z, Seftor EA, Pemberton PA and Hendrix MJ: Maspin: Molecular mechanisms and therapeutic implications. Cancer Metastasis Rev. 31:529–551. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Zou Z, Anisowicz A, Hendrix MJ, Thor A, Neveu M, Sheng S, Rafidi K, Seftor E and Sager R: Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science. 263:526–529. 1994. View Article : Google Scholar : PubMed/NCBI

15 

Cai Z, Zhou Y, Lei T, Chiu JF and He QY: Mammary serine protease inhibitor inhibits epithelial growth factor-induced epithelial-mesenchymal transition of esophageal carcinoma cells. Cancer. 115:36–48. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Wu S, Yu L, Cheng Z, Song W, Zhou L and Tao Y: Expression of maspin in non-small cell lung cancer and its relationship to vasculogenic mimicry. J Huazhong Univ Sci Technolog Med Sci. 32:346–352. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Zhang W, Shi HY and Zhang M: Maspin overexpression modulates tumor cell apoptosis through the regulation of Bcl-2 family proteins. BMC Cancer. 5:502005. View Article : Google Scholar : PubMed/NCBI

18 

Li X, Chen D, Yin S, Meng Y, Yang H, Landis-Piwowar KR, Li Y, Sarkar FH, Reddy GP, Dou QP, et al: Maspin augments proteasome inhibitor-induced apoptosis in prostate cancer cells. J Cell Physiol. 212:298–306. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Dzinic SH, Chen K, Thakur A, Kaplun A, Bonfil RD, Li X, Liu J, Bernardo MM, Saliganan A, Back JB, et al: Maspin expression in prostate tumor elicits host anti-tumor immunity. Oncotarget. 5:11225–11236. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Reis-Filho JS, Torio B, Albergaria A and Schmitt FC: Maspin expression in normal skin and usual cutaneous carcinomas. Virchows Arch. 441:551–558. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Abdou AG, Maraee AH, El-Monaem Shoeib MA and Abo Saida AM: Maspin expression in epithelial skin tumours: An immunohistochemical study. J Cutan Aesthet Surg. 4:111–117. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Wang JH, Zhang L, Ma YW, Xiao J, Zhang Y, Liu M and Tang H: microRNA-34a-upregulated retinoic acid-inducible gene-I promotes apoptosis and delays cell cycle transition in cervical cancer cells. DNA Cell Biol. 35:267–279. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Cassarino DS, Derienzo DP and Barr RJ: Cutaneous squamous cell carcinoma: A comprehensive clinicopathologic classification. Part one. J Cutan Pathol. 33:191–206. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Cassarino DS, Derienzo DP and Barr RJ: Cutaneous squamous cell carcinoma: A comprehensive clinicopathologic classification - part two. J Cutan Pathol. 33:261–279. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Demchenko AP: The change of cellular membranes on apoptosis: Fluorescence detection. Exp Oncol. 34:263–268. 2012.PubMed/NCBI

27 

Bounda GA, Zhou W, Wang DD and Yu F: Rhein elicits in vitro cytotoxicity in primary human liver HL-7702 cells by inducing apoptosis through mitochondria-mediated pathway. Evid Based Complement Alternat Med. 2015:3298312015. View Article : Google Scholar : PubMed/NCBI

28 

Nicholson DW and Thornberry NA: Caspases: Killer proteases. Trends Biochem Sci. 22:299–306. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Yi CH and Yuan J: The Jekyll and Hyde functions of caspases. Dev Cell. 16:21–34. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Sarvothaman S, Undi RB, Pasupuleti SR, Gutti U and Gutti RK: Apoptosis: Role in myeloid cell development. Blood Res. 50:73–79. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Snigdha S, Smith ED, Prieto GA and Cotman CW: Caspase-3 activation as a bifurcation point between plasticity and cell death. Neurosci Bull. 28:14–24. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Gerö D and Szabó C: Poly(ADP-ribose) polymerase: A new therapeutic target? Curr Opin Anaesthesiol. 21:111–121. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, Roth KA, MacGregor GR, Thompson CB and Korsmeyer SJ: Proapoptotic BAX and BAK: A requisite gateway to mitochondrial dysfunction and death. Science. 292:727–730. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Besbes S, Mirshahi M, Pocard M and Billard C: New dimension in therapeutic targeting of BCL-2 family proteins. Oncotarget. 6:12862–12871. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Danial NN and Korsmeyer SJ: Cell death: Critical control points. Cell. 116:205–219. 2004. View Article : Google Scholar : PubMed/NCBI

36 

Nagata Y, Nagahisa H, Aida Y, Okutomi K, Nagasawa T and Todokoro K: Thrombopoietin induces megakaryocyte differentiation in hematopoietic progenitor FDC-P2 cells. J Biol Chem. 270:19673–19675. 1995. View Article : Google Scholar : PubMed/NCBI

37 

Zou Z, Gao C, Nagaich AK, Connell T, Saito S, Moul JW, Seth P, Appella E and Srivastava S: p53 regulates the expression of the tumor suppressor gene maspin. J Biol Chem. 275:6051–6054. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Eitel JA, Bijangi-Vishehsaraei K, Saadatzadeh MR, Bhavsar JR, Murphy MP, Pollok KE and Mayo LD: PTEN and p53 are required for hypoxia induced expression of maspin in glioblastoma cells. Cell Cycle. 8:896–901. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Chen WS, Yen CJ, Chen YJ, Chen JY, Wang LY, Chiu SJ, Shih WL, Ho CY, Wei TT, Pan HL, et al: miRNA-7/21/107 contribute to HBx-induced hepatocellular carcinoma progression through suppression of maspin. Oncotarget. 6:25962–25974. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Yuan K, Xie K, Fox J, Zeng H, Gao H, Huang C and Wu M: Decreased levels of miR-224 and the passenger strand of miR-221 increase MBD2, suppressing maspin and promoting colorectal tumor growth and metastasis in mice. Gastroenterology. 145:853–864, e859. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu H, Mao Q, Liu W, Yang Z, Jian X, Qu L and He C: Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells. Oncol Rep 37: 2875-2882, 2017.
APA
Zhu, H., Mao, Q., Liu, W., Yang, Z., Jian, X., Qu, L., & He, C. (2017). Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells. Oncology Reports, 37, 2875-2882. https://doi.org/10.3892/or.2017.5574
MLA
Zhu, H., Mao, Q., Liu, W., Yang, Z., Jian, X., Qu, L., He, C."Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells". Oncology Reports 37.5 (2017): 2875-2882.
Chicago
Zhu, H., Mao, Q., Liu, W., Yang, Z., Jian, X., Qu, L., He, C."Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells". Oncology Reports 37, no. 5 (2017): 2875-2882. https://doi.org/10.3892/or.2017.5574
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu H, Mao Q, Liu W, Yang Z, Jian X, Qu L and He C: Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells. Oncol Rep 37: 2875-2882, 2017.
APA
Zhu, H., Mao, Q., Liu, W., Yang, Z., Jian, X., Qu, L., & He, C. (2017). Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells. Oncology Reports, 37, 2875-2882. https://doi.org/10.3892/or.2017.5574
MLA
Zhu, H., Mao, Q., Liu, W., Yang, Z., Jian, X., Qu, L., He, C."Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells". Oncology Reports 37.5 (2017): 2875-2882.
Chicago
Zhu, H., Mao, Q., Liu, W., Yang, Z., Jian, X., Qu, L., He, C."Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells". Oncology Reports 37, no. 5 (2017): 2875-2882. https://doi.org/10.3892/or.2017.5574
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team