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Article

The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway

  • Authors:
    • Yan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, Binzhou Tuberculosis Prevention and Control Hospital, Huimin, Binzhou, Shandong 251700, P.R. China
  • Pages: 3520-3526
    |
    Published online on: May 2, 2017
       https://doi.org/10.3892/or.2017.5618
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Abstract

In the present study, the role of microRNA‑15a (miR‑15a) was investigated in hepatitis B virus (HBV)‑associated liver cancer. The results revealed that the expression levels of miR-15a were increased in HBV-associated liver cancer tissues compared with the levels in normal tumor‑adjacent tissues. Moreover, Smad-7 protein expression in patients with HBV-associated liver cancer was higher than that in normal tumor-adjacent tissues. In addition, miR-15a expression and Smad-7 protein expression were increased in HepG2 hepatocellular carcinoma cells compared with that noted in L-02 normal hepatocytes. In HepG2 cells, miR-15a inhibition suppressed cell proliferation and increased Smad-7 protein expression. The inhibition of miR-15a was also demonstrated to decrease transforming growth factor (TGF)-β1 protein expression and Smad-2, p-Smad-2 and Smad-4 expression levels in HepG2 cells. Furthermore, FSP1 protein expression and caspase-3/-7 activities were enhanced by miR-15a inhibition in HepG2 cells compared with the control group. Treatment with recombinant TGF-β1 was demonstrated to activate Smad‑2/-4 and FSP1 protein expression and increase caspase-3/-7 activity in HepG2 cells. Collectively, these findings demonstrate that the miR-15a/Smad-7/TGF-β pathway is important in HBV-associated liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y: The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway. Oncol Rep 37: 3520-3526, 2017.
APA
Wang, Y. (2017). The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway. Oncology Reports, 37, 3520-3526. https://doi.org/10.3892/or.2017.5618
MLA
Wang, Y."The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway". Oncology Reports 37.6 (2017): 3520-3526.
Chicago
Wang, Y."The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway". Oncology Reports 37, no. 6 (2017): 3520-3526. https://doi.org/10.3892/or.2017.5618
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y: The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway. Oncol Rep 37: 3520-3526, 2017.
APA
Wang, Y. (2017). The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway. Oncology Reports, 37, 3520-3526. https://doi.org/10.3892/or.2017.5618
MLA
Wang, Y."The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway". Oncology Reports 37.6 (2017): 3520-3526.
Chicago
Wang, Y."The inhibition of microRNA-15a suppresses hepatitis B virus-associated liver cancer cell growth through the Smad/TGF-β pathway". Oncology Reports 37, no. 6 (2017): 3520-3526. https://doi.org/10.3892/or.2017.5618
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