Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells

  • Authors:
    • Wei Li
    • Ying Zhou
    • Jin Yang
    • Haining Li
    • Huanhuan Zhang
    • Ping Zheng
  • View Affiliations

  • Published online on: May 11, 2017     https://doi.org/10.3892/or.2017.5637
  • Pages: 3459-3466
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Abstract

Curcumin possesses an anticancer effect against a wide assortment of tumors with selective cytotoxicity for tumor cells. However, the mechanism involved in the curcumin‑induced anticancer effect remain unclear. In the present study, we investigated the efficacy of curcumin against human gastric cancer cell growth and the molecular mechanism involved. Our results demonstrated that curcumin inhibited the viabilities of gastric cancer cell lines BGC-823, SGC-7901 and MKN-28 in both a time- and dose-dependent manner. In addition, curcumin treatment induced gastric cancer cell apoptosis in a dose‑responsive manner. Western blotting of apoptosis‑related proteins further confirmed the pro-apoptotic potential of curcumin. After exposure to curcumin, a robust induction of autophagy was observed in gastric cancer cells, which was characterized by the formation of acidic vesicular organelles (AVOs), conversion of LC3-I to LC3-II and an increase in the levels of autophagy‑related proteins. Activation of the PI3K/Akt/mTOR signaling pathway was suppressed in gastric cancer cells with curcumin treatment. However, administration of the autophagy inhibitor 3-methyladenine (3-MA) significantly promoted the apoptotic cell death induced by curcumin. Collectively, our findings provide new evidence that curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells in vitro. Autophagy inhibitor treatment may provide a novel and effective strategy for improving the anticancer effect of curcumin against gastric cancer.
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June-2017
Volume 37 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li W, Zhou Y, Yang J, Li H, Zhang H and Zheng P: Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells. Oncol Rep 37: 3459-3466, 2017
APA
Li, W., Zhou, Y., Yang, J., Li, H., Zhang, H., & Zheng, P. (2017). Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells. Oncology Reports, 37, 3459-3466. https://doi.org/10.3892/or.2017.5637
MLA
Li, W., Zhou, Y., Yang, J., Li, H., Zhang, H., Zheng, P."Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells". Oncology Reports 37.6 (2017): 3459-3466.
Chicago
Li, W., Zhou, Y., Yang, J., Li, H., Zhang, H., Zheng, P."Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells". Oncology Reports 37, no. 6 (2017): 3459-3466. https://doi.org/10.3892/or.2017.5637