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Article

Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5

  • Authors:
    • Weibo Jiang
    • Jia Zheng
    • Tao Yu
    • Jincheng Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, P.R. China
  • Pages: 1099-1107
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    Published online on: June 13, 2017
       https://doi.org/10.3892/or.2017.5715
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Abstract

It has been suggested that microRNAs (miRNAs) act as critical regulators in tumorigenesis. MicroRNA-495 (miR-495) has been suggested as a cancer-associated miRNA in various types of cancers; however, the role of miR-495 in osteosarcoma is unknown. The aim of the present study was to determine whether miR-495 is involved in osteosarcoma, and to investigate the potential molecular mechanism of its involvement. We found that miR-495 was significantly downregulated in osteosarcoma tissues and cell lines, as detected by real-time quantitative polymerase chain reaction (RT-qPCR). Overexpression of miR-495 inhibited osteosarcoma cell proliferation in 3-(4,5-dimethylthiazol-2-yl)- ,5-diphenyltetrazolium bromide, colony formation and cell cycle assays. Overexpression of miR-495 induced osteosarcoma cell apoptosis. Moreover, miR-495 overexpression also inhibited osteosarcoma cell invasion. Bioinformatics and luciferase reporter assays demonstrated that miR-495 targets the 3'-untranslated region of high-mobility group nucleosome‑binding domain 5 (HMGN5), a potential oncogene in various types of cancers. Overexpression of miR-495 inhibited the expression of HMGN5, cyclin B1, Bcl-2 and matrix metalloproteinase 9. In addition, restoration of HMGN5 protein expression abrogated the miR-495-induced effects. Taken together, the present study indicated that miR-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting HMGN5, providing a novel insight into the molecular pathogenesis of osteosarcoma and suggesting a potential molecular target for the development of an miRNA-targeted therapeutic strategy for osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang W, Zheng J, Yu T and Wang J: Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5. Oncol Rep 38: 1099-1107, 2017.
APA
Jiang, W., Zheng, J., Yu, T., & Wang, J. (2017). Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5. Oncology Reports, 38, 1099-1107. https://doi.org/10.3892/or.2017.5715
MLA
Jiang, W., Zheng, J., Yu, T., Wang, J."Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5". Oncology Reports 38.2 (2017): 1099-1107.
Chicago
Jiang, W., Zheng, J., Yu, T., Wang, J."Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5". Oncology Reports 38, no. 2 (2017): 1099-1107. https://doi.org/10.3892/or.2017.5715
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang W, Zheng J, Yu T and Wang J: Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5. Oncol Rep 38: 1099-1107, 2017.
APA
Jiang, W., Zheng, J., Yu, T., & Wang, J. (2017). Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5. Oncology Reports, 38, 1099-1107. https://doi.org/10.3892/or.2017.5715
MLA
Jiang, W., Zheng, J., Yu, T., Wang, J."Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5". Oncology Reports 38.2 (2017): 1099-1107.
Chicago
Jiang, W., Zheng, J., Yu, T., Wang, J."Overexpression of microRNA-495 suppresses the proliferation and invasion and induces the apoptosis of osteosarcoma cells by targeting high-mobility group nucleosome-binding domain 5". Oncology Reports 38, no. 2 (2017): 1099-1107. https://doi.org/10.3892/or.2017.5715
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