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Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway

  • Authors:
    • Wenyan Tian
    • Jiaqing Shen
    • Weichang Chen
  • View Affiliations / Copyright

    Affiliations: The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 745-754
    |
    Published online on: June 22, 2017
       https://doi.org/10.3892/or.2017.5743
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Abstract

Midkine (MK) is reported to be associated with the clinical stages and distant metastases in gastric cancer, and to positively regulate the proliferation of human gastric cancer cells. However, the possible mechanisms of MK in the development of gastric cancer are still not fully clarified. In this study, the therapeutic effect of MK inhibition in gastric cancer in vivo and in vitro was investigated, by knock-down of MK expression with a small interfering RNA (siRNA). MK was expressed in gastric carcinoma tissues and cancer cells. The cytotoxic effect of cisplatin on AGS cells in vitro was attenuated by recombinant human MK, but was promoted by suppressing MK expression via downregulating the Notch signaling pathway-related proteins (Notch 1, Notch 2, Delta‑like 1 and Jagged 1). Suppression of MK expression also promoted the inhibitory effect of cisplatin on AGS cells in vivo. In concusion, suppression of midkine gene promoted the antitumoral effect of cisplatin on human gastric cell line AGS in vitro and in vivo via Notch signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Tian W, Shen J and Chen W: Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway. Oncol Rep 38: 745-754, 2017.
APA
Tian, W., Shen, J., & Chen, W. (2017). Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway. Oncology Reports, 38, 745-754. https://doi.org/10.3892/or.2017.5743
MLA
Tian, W., Shen, J., Chen, W."Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway". Oncology Reports 38.2 (2017): 745-754.
Chicago
Tian, W., Shen, J., Chen, W."Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway". Oncology Reports 38, no. 2 (2017): 745-754. https://doi.org/10.3892/or.2017.5743
Copy and paste a formatted citation
x
Spandidos Publications style
Tian W, Shen J and Chen W: Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway. Oncol Rep 38: 745-754, 2017.
APA
Tian, W., Shen, J., & Chen, W. (2017). Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway. Oncology Reports, 38, 745-754. https://doi.org/10.3892/or.2017.5743
MLA
Tian, W., Shen, J., Chen, W."Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway". Oncology Reports 38.2 (2017): 745-754.
Chicago
Tian, W., Shen, J., Chen, W."Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway". Oncology Reports 38, no. 2 (2017): 745-754. https://doi.org/10.3892/or.2017.5743
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