CtBP2 is associated with angiogenesis and regulates the apoptosis of prostate cancer cells

Xuan,Qiang; Zhong,Xiaoge; Li,Weidong; Mo,Zengnan; Huang,Yuanjie; Hu,Yanling

doi:10.3892/or.2017.5763

CtBP2 is associated with angiogenesis and regulates the apoptosis of prostate cancer cells

Authors:
- Qiang Xuan
- Xiaoge Zhong
- Weidong Li
- Zengnan Mo
- Yuanjie Huang
- Yanling Hu
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Affiliations: Department of Urology, Anhui Provincial Hospital Affiliated Anhui Medical University, Hefei, Anhui 230022, P.R. China, Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Life Sciences Institute, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: June 28, 2017 https://doi.org/10.3892/or.2017.5763
Pages: 1259-1267

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Abstract

Angiogenesis is associated with prostate cancer (PCa) development and progression. Aberrant expression of C-terminal binding protein (CtBP)2 has been observed in PCa, but whether its change in expression plays a significant role in angiogenesis has not been completely characterized. we attempted to integrate and analyze the genome-wide association study (GWAS) of follicle stimulating hormone receptor (FSHR) and CtBP2, the Cancer Genome Atlas (TCGA) data and CtBP2 binding data in CistromeMap (18) to explore the mechanism of CtBP2 in PCa, and performed pathway enrichment analysis. We revealed that the top 6 pathways were closely related with angiogenesis. We used siRNA and overexpression plasmids to silence and overexpress CtBP2 expression. Altered expression of CtBP2 affected the expression of VEGFA, FSHR, FHL2 and SMAD3 which are closely related with angiogenesis. In addition, silencing of CtBP2 markedly increased the apoptosis of PCa cells in vitro, and decreased the expression of IL-8, AT2R, CCND1 and MMP9 which are associated with cancer progression. These results highlight the association between CtBP2 and angiogenesis in PCa and indicate that CtBP2 may be a potential therapeutic target for PCa.

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