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Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells

  • Authors:
    • Siyue Du
    • Wenjiao Qin
    • Haiyan Leng
    • Zi Chen
    • Tao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Huashan Hospital Affiliated to Fudan University, Jingan, Shanghai, P.R. China, Department of Laboratory Medicine, Huashan Hospital Affiliated to Fudan University, Jingan, Shanghai, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 809-818
    |
    Published online on: June 30, 2017
       https://doi.org/10.3892/or.2017.5770
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Abstract

Multiple myeloma (MM) is one of the most common malignant blood cancers. Previous studies have reported that proteasome 26S subunit non-ATPase 10 (PSMD10) is an oncoprotein with complex roles in hepatocellular carcinoma and other malignant tumors. Notably, research on the relationship between PSMD10 and tumorigenesis of MM has rarely been reported. The present study was designed to explore the possibility of PSMD10 as a therapeutic target in the treatment of MM, and the use of RNA interference (RNAi) to determine the function PSMD10. A recombinant lentivirus-mediated short hairpin RNA (shRNA) targeting human PSMD10 mRNA was constructed and used to decrease endogenous PSMD10 expression in the MM RPMI-8226 cell line in vitro. Expression of the PSMD10-targeting shRNA in RPMI-8226 cells transduced with the recombinant vector could be tracked by observing the expression of green fluorescent protein after infection. A transient transgenic RPMI-8226 cell line was generated by transducing cells with the packaged viral particles. Western blot analysis indicated that the protein levels of PSMD10 in the PSMD10-shRNA MM cells were significantly lower than those in the cells transduced with the negative control shRNA. Notably, RT-qPCR analysis did not reveal a marked change in the PSMD10 mRNA level; thus, the knockdown effect of the PSMD10-shRNA may occur during translation. Furthermore, apoptosis of MM cells was increased by silencing PSMD10 expression. Overall, the results demonstrated that the lentivirus-mediated shRNA vector-based RNAi expression system is an efficient method to silence PSMD10 gene expression in the MM RPMI-8226 cell line. It may provide a basis to study the role of PSMD10 in tumor cells, and may be a reliable gene therapy strategy in the clinic.
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View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI

2 

National Cancer Institute SEER database: Surveillance, Epidemiology, and End Results Program. 2014.(cited June 30). http://seer.cancer.gov/

3 

Becker N: Epidemiology of multiple myeloma. Multiple Myeloma: Recent Results in Cancer Research. Moehler T and Goldschmid H: Berlin, Heidelberg, Germany: Springer-Verlag; pp. 25–35. 2011, View Article : Google Scholar

4 

Bergsagel PL: Epidemiology, etiology, and molecularpathogenesis. Multiple Myeloma. Richardson PG and Anderson KC: London, Chicago: Remedica Publi-shing; pp. 1–24. 2004

5 

Liu Y, Xu J, Jiang M, Ni L, Chen Y and Ling Y: Association between functional PSMD10 Rs111638916 variant regulated by MiR-505 and gastric cancer risk in a Chinese population. Cell Physiol Biochem. 37:1010–1017. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Uddin MH, Choi MH, Kim WH, Jang JJ and Hong ST: Involvement of PSMD10, CDK4, and tumor suppressors in development of intrahepatic cholangiocarcinoma of Syrian Golden Hamsters induced by Clonorchis sinensis and N-nitrosodimethylamine. PLoS Negl Trop Dis. 9:e00040082015. View Article : Google Scholar : PubMed/NCBI

7 

Li J, Tian F, Li D, Chen J, Jiang P, Zheng S, Li X and Wang S: MiR-605 represses PSMD10/Gankyrin and inhibits intrahepatic cholangiocarcinoma cell progression. FEBS Lett. 588:3491–3500. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Chen BF, Suen YK, Gu S, Li L and Chan WY: A miR-199a/miR-214 self-regulatory network via PSMD10, TP53 and DNMT1 in testicular germ cell tumor. Sci Rep. 4:64132014. View Article : Google Scholar : PubMed/NCBI

9 

Luo T, Fu J, Xu A, Su B, Ren Y, Li N, Zhu J, Zhao X, Dai R, Cao J, et al: PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy. 12:1355–1371. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Yu T, Tao Y, Yang M, Chen P, Gao X, Zhang Y, Zhang T, Chen Z, Hou J, Zhang Y, et al: Profiling human protein degradome delineates cellular responses to proteasomal inhibition and reveals a feedback mechanism in regulating proteasome homeostasis. Cell Res. 24:1214–1230. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Tomko RJ Jr, Funakoshi M, Schneider K, Wang J and Hochstrasser M: Heterohexameric ring arrangement of the eukaryotic proteasomal ATPases: Implications for proteasome structure and assembly. Mol Cell. 38:393–403. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Higashitsuji H, Itoh K, Nagao T, Dawson S, Nonoguchi K, Kido T, Mayer RJ, Arii S and Fujita J: Reduced stability of retinoblastoma protein by gankyrin, an oncogenic ankyrin-repeat protein overexpressed in hepatomas. Nat Med. 6:96–99. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Higashitsuji H, Higashitsuji H, Itoh K, Sakurai T, Nagao T, Sumitomo Y, Masuda T, Dawson S, Shimada Y, Mayer RJ, et al: The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53. Cancer Cell. 8:75–87. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Bai Z, Tai Y, Li W, Zhen C, Gu W, Jian Z, Wang Q, Lin JE, Zhao Q, Gong W, et al: Gankyrin activates IL-8 to promote hepatic metastasis of colorectal cancer. Cancer Res. 73:4548–4558. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Zhen C, Chen L, Zhao Q, Liang B, Gu YX, Bai ZF, Wang K, Xu X, Han QY, Fang DF, et al: Gankyrin promotes breast cancer cell metastasis by regulating Rac1 activity. Oncogene. 32:3452–3460. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Meng Y, He L, Guo X, Tang S, Zhao X, Du R, Jin J, Bi Q, Li H, Nie Y, et al: Gankyrin promotes the proliferation of human pancreatic cancer. Cancer Lett. 297:9–17. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Misiewicz-Krzeminska I, Sarasquete ME, Quwaider D, Krzeminski P, Ticona FV, Paíno T, Delgado M, Aires A, Ocio EM, García-Sanz R, et al: Restoration of microRNA-214 expression reduces growth of myeloma cells through positive regulation of P53 and inhibition of DNA replication. Haematologica. 98:640–648. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K and Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494–498. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Karagiannis TC and El-Osta A: siRNAs: Mechanism of RNA interference, in vivo and potential clinical applications. Cancer Biol Ther. 3:1069–1074. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Brummelkamp TR, Bernards R and Agami R: A system for stable expression of short interfering RNAs in mammalian cells. Science. 296:550–553. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Paddison PJ, Caudy AA, Bernstein E, Hannon GJ and Conklin DS: Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16:948–958. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS and Khvorova A: Rational siRNA design for RNA interference. Nat Biotechnol. 22:326–330. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Barøy T, Sørensen K, Lindeberg MM and Frengen E: shRNA expression constructs designed directly from siRNA oligonucleotide sequences. Mol Biotechnol. 45:116–120. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Kumar SK, Rajkumar SV, Dispenzieri A, Lacy MQ, Hayman SR, Buadi FK, Zeldenrust SR, Dingli D, Russell SJ, Lust JA, et al: Improved survival in multiple myeloma and the impact of novel therapies. Blood. 111:2516–2520. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Rovira M, Rosinol L, Martinez C, Fernández-Avilés F and Blade J: Is there a curative potential of autologous stem cell transplantation in multiple myeloma? Long-term results from a single-centre series. Bone Marrow Transplant. 42:S147(abstract P592). 2009.

26 

Gahrton G, Svensson H, Cavo M, Apperly J, Bacigalupo A, Björkstrand B, Bladé J, Cornelissen J, de Laurenzi A, Facon T, et al: European Group for Blood and Marrow Transplantation: Progress in allogenic bone marrow and peripheral blood stem cell transplantation for multiple myeloma: A comparison between transplants performed 1983–93 and 1994–98 at European Group for Blood and Marrow Transplantation centres. Br J Haematol. 113:209–216. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Kyle RA and Rajkumar SV: Multiple myeloma. Blood. 111:2962–2972. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Adams J: The proteasome: A suitable antineoplastic target. Nat Rev Cancer. 4:349–360. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Bianchi G, Oliva L, Cascio P, Pengo N, Fontana F, Cerruti F, Orsi A, Pasqualetto E, Mezghrani A, Calbi V, et al: The proteasome load versus capacity balance determines apoptotic sensitivity of multiple myeloma cells to proteasome inhibition. Blood. 113:3040–3049. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Meister S, Schubert U, Neubert K, Herrmann K, Burger R, Gramatzki M, Hahn S, Schreiber S, Wilhelm S, Herrmann M, et al: Extensive immunoglobulin production sensitizes myeloma cells for proteasome inhibition. Cancer Res. 67:1783–1792. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Ortiz-Navarrete V, Seelig A, Gernold M, Frentzel S, Kloetzel PM and Hämmerling GJ: Subunit of the 20S proteasome (multicatalytic proteinase) encoded by the major histocompatibility complex. Nature. 353:662–664. 1991. View Article : Google Scholar : PubMed/NCBI

32 

Rivett AJ, Bose S, Brooks P and Broadfoot KI: Regulation of proteasome complexes by gamma-interferon and phosphorylation. Biochimie. 83:363–366. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Niewerth D, Jansen G, Assaraf YG, Zweegman S, Kaspers GJ and Cloos J: Molecular basis of resistance to proteasome inhibitors in hematological malignancies. Drug Resist Updat. 18:18–35. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Agarwal AK, Xing C, DeMartino GN, Mizrachi D, Hernandez MD, Sousa AB, de Martínez Villarreal L, dos Santos HG and Garg A: PSMB8 encoding the β5i proteasome subunit is mutated in joint contractures, muscle atrophy, microcytic anemia, and panniculitis-induced lipodystrophy syndrome. Am J Hum Genet. 87:866–872. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Kincaid EZ, Che JW, York I, Escobar H, Reyes-Vargas E, Delgado JC, Welsh RM, Karow ML, Murphy AJ, Valenzuela DM, et al: Mice completely lacking immunoproteasomes show major changes in antigen presentation. Nat Immunol. 13:129–135. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Chondrogianni N, Stratford FLL, Trougakos IP, Friguet B, Rivett AJ and Gonos ES: Central role of the proteasome in senescence and survival of human fibroblasts: Induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation. J Biol Chem. 278:28026–28037. 2003. View Article : Google Scholar : PubMed/NCBI

37 

Chondrogianni N, Tzavelas C, Pemberton AJ, Nezis IP, Rivett AJ and Gonos ES: Overexpression of proteasome beta5 assembled subunit increases the amount of proteasome and confers ameliorated response to oxidative stress and higher survival rates. J Biol Chem. 280:11840–11850. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Franke NE, Niewerth D, Assaraf YG, van Meerloo J, Vojtekova K, van Zantwijk CH, Zweegman S, Chan ET, Kirk CJ, Geerke DP, et al: Impaired bortezomib binding to mutant β5 subunit of the proteasome is the underlying basis for bortezomib resistance in leukemia cells. Leukemia. 26:757–768. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Blackburn C, Gigstad KM, Hales P, Garcia K, Jones M, Bruzzese FJ, Barrett C, Liu JX, Soucy TA, Sappal DS, et al: Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S beta5-subunit. Biochem J. 430:461–476. 2010. View Article : Google Scholar : PubMed/NCBI

40 

Rückrich T, Kraus M, Gogel J, Beck A, Ovaa H, Verdoes M, Overkleeft HS, Kalbacher H and Driessen C: Characterization of the ubiquitin-proteasome system in bortezomib-adapted cells. Leukemia. 23:1098–1105. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Niewerth D, Franke NE, Jansen G, Assaraf YG, van Meerloo J, Kirk CJ, Degenhardt J, Anderl J, Schimmer AD, Zweegman S, et al: Higher ratio immune versus constitutive proteasome level as novel indicator of sensitivity of pediatric acute leukemia cells to proteasome inhibitors. Haematologica. 98:1896–1904. 2013.b. View Article : Google Scholar : PubMed/NCBI

42 

Heckmann D, Laufs S, Maier P, Zucknick M, Giordano FA, Veldwijk MR, Eckstein V, Wenz F, Zeller WJ, Fruehauf S, et al: A lentiviral CXCR4 overexpression and knockdown model in colorectal cancer cell lines reveals plerixafor-dependent suppression of SDF-1α-induced migration and invasion. Onkologie. 34:502–508. 2011. View Article : Google Scholar : PubMed/NCBI

43 

Christoph T, Bahrenberg G, De Vry J, Englberger W, Erdmann VA, Frech M, Kögel B, Röhl T, Schiene K, Schröder W, et al: Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice. Mol Cell Neurosci. 37:579–589. 2008. View Article : Google Scholar : PubMed/NCBI

44 

Sacca R, Engle SJ, Qin W, Stock JL and McNeish JD: Genetically engineered mouse models in drug discovery research. Methods Mol Biol. 602:37–54. 2010. View Article : Google Scholar : PubMed/NCBI

45 

Lozano G and Zambetti GP: Gankyrin: An intriguing name for a novel regulator of p53 and RB. Cancer Cell. 8:3–4. 2005. View Article : Google Scholar : PubMed/NCBI

46 

Umemura A, Itoh Y, Itoh K, Yamaguchi K, Nakajima T, Higashitsuji H, Onoue H, Fukumoto M, Okanoue T and Fujita J: Association of gankyrin protein expression with early clinical stages and insulin-like growth factor-binding protein 5 expression in human hepatocellular carcinoma. Hepatology. 47:493–502. 2008. View Article : Google Scholar : PubMed/NCBI

47 

Ooi MG, Hayden PJ, Kotoula V, McMillin DW, Charalambous E, Daskalaki E, Raje NS, Munshi NC, Chauhan D, Hideshima T, et al: Interactions of the Hdm2/p53 and proteasome pathways may enhance the antitumor activity of bortezomib. Clin Cancer Res. 15:7153–7160. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Du S, Qin W, Leng H, Chen Z and Zhang T: Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells. Oncol Rep 38: 809-818, 2017.
APA
Du, S., Qin, W., Leng, H., Chen, Z., & Zhang, T. (2017). Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells. Oncology Reports, 38, 809-818. https://doi.org/10.3892/or.2017.5770
MLA
Du, S., Qin, W., Leng, H., Chen, Z., Zhang, T."Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells". Oncology Reports 38.2 (2017): 809-818.
Chicago
Du, S., Qin, W., Leng, H., Chen, Z., Zhang, T."Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells". Oncology Reports 38, no. 2 (2017): 809-818. https://doi.org/10.3892/or.2017.5770
Copy and paste a formatted citation
x
Spandidos Publications style
Du S, Qin W, Leng H, Chen Z and Zhang T: Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells. Oncol Rep 38: 809-818, 2017.
APA
Du, S., Qin, W., Leng, H., Chen, Z., & Zhang, T. (2017). Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells. Oncology Reports, 38, 809-818. https://doi.org/10.3892/or.2017.5770
MLA
Du, S., Qin, W., Leng, H., Chen, Z., Zhang, T."Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells". Oncology Reports 38.2 (2017): 809-818.
Chicago
Du, S., Qin, W., Leng, H., Chen, Z., Zhang, T."Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI‑8226 multiple myeloma cells". Oncology Reports 38, no. 2 (2017): 809-818. https://doi.org/10.3892/or.2017.5770
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