MicroRNA and target mRNA selection through invasion and cytotoxicity cell modeling and bioinformatics approaches in esophageal squamous cell carcinoma

Lu,Mu; Song,Yaqin; Fu,Wenbo; Liu,Yang; Huai,Shitao; Cui,Xiaobin; Pang,Lijuan; Yang,Lan; Wei,Yutao

doi:10.3892/or.2017.5776

MicroRNA and target mRNA selection through invasion and cytotoxicity cell modeling and bioinformatics approaches in esophageal squamous cell carcinoma

Authors:
- Mu Lu
- Yaqin Song
- Wenbo Fu
- Yang Liu
- Shitao Huai
- Xiaobin Cui
- Lijuan Pang
- Lan Yang
- Yutao Wei
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Affiliations: Medical College of Shihezi University, Shihezi, Xinjiang, P.R. China, Department of Nutrition, Jining No. 1 People's Hospital, Jining, Shandong, P.R. China, Department of Cardiology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, P.R. China, Department of Pathology, Medical College of Shihezi University, Shihezi, Xinjiang, P.R. China, Department of Thoracic and Cardiovascular Surgery, First Hospital Affiliated to Medical College of Shihezi University, Shihezi, Xinjiang, P.R. China
Published online on: June 30, 2017 https://doi.org/10.3892/or.2017.5776
Pages: 1181-1189

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Abstract

This study analyzed microRNA (miRNA) and mRNA expression profiles and investigated the biological characteristics of ESCC by using invasion and cytotoxicity cell models. miRNA profiles were evaluated through miRNA microarray. Transwell chamber and nedaplatin (NDP) were used to construct invasion and cytotoxicity cell models. Invasion Transwell and cytotoxicity assays were performed to examine the invasiveness and proliferation in the cell models. Functional miRNAs were selected from dysregulated miRNAs through qRT-PCR. Biometric Research Program (BRB)-array tools, Cytoscape plugins, and DAVID were utilized to find potential mRNAs targeted by these two miRNAs between ESCC and paired normal adjacent tissues. Our microarray obtained 11 dysregulated miRNAs expressed in three paired ESCC samples from Kazakhs (ethnicity in Northwestern China). qRT-PCR demonstrated the miRNA expression in the invasion and cytotoxicity cell models. miR‑652-5p and miR‑21‑5p exhibited a consistent expression level in the microarray and cell models. Bioinformatics revealed that the potential targets of PLD1, MSH2, STC1, and DSG1 might be involved in ESCC invasion and proliferation. Cell models with bioinformatics approaches may help distinguish functional genes. miR‑652-5p, miR‑21‑5p, and their potential target genes may participate in ESCC development and metastasis.

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