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Article

Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway

  • Authors:
    • Ran Tao
    • Wen-Yi Sun
    • De-Hai Yu
    • Wei Qiu
    • Wei-Qun Yan
    • Yan-Hua Ding
    • Guang-Yi Wang
    • Hai-Jun Li
  • View Affiliations / Copyright

    Affiliations: Institute of Translational Medicine, the First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Clinical Pharmacy and Pharmaceutical Management, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Cancer Center, the First Hospital of Jilin University, Changchun, Jilin 130061, P.R. China, Department of Surgery, the First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Phase Ⅰ Clinical Research Center, the First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 1233-1239
    |
    Published online on: July 3, 2017
       https://doi.org/10.3892/or.2017.5779
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Abstract

The function of sodium cantharidinate on inducing hepatocellular carcinoma cell apoptosis was investigated for the first time. Sodium cantharidinate inhibits HepG2 cell growth mainly by LC3 autophagy pathway. MTT results show that sodium cantharidinate effectively inhibits the proliferation of HepG2 cells in a dose- and time-dependent manner and induce cell apoptosis by caspase-3 activity. The further western blotting and FACS detection show that sodium cantharidinate initiates HepG2 cell autophagy program by LC3 pathway. Autophagy-specific inhibitor 3-MA reduce sodium cantharidinate-induced caspase-3 activity and HepG2 cell apoptosis. Silence of the LC3 gene in HepG2 cell lines also reduce sodium cantharidinate-induced cell apoptosis. Collectively, our data indicate that sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway. Sodium cantharidinate has potential for development as a new drug for treatment of human HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Tao R, Sun W, Yu D, Qiu W, Yan W, Ding Y, Wang G and Li H: Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway . Oncol Rep 38: 1233-1239, 2017.
APA
Tao, R., Sun, W., Yu, D., Qiu, W., Yan, W., Ding, Y. ... Li, H. (2017). Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway . Oncology Reports, 38, 1233-1239. https://doi.org/10.3892/or.2017.5779
MLA
Tao, R., Sun, W., Yu, D., Qiu, W., Yan, W., Ding, Y., Wang, G., Li, H."Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway ". Oncology Reports 38.2 (2017): 1233-1239.
Chicago
Tao, R., Sun, W., Yu, D., Qiu, W., Yan, W., Ding, Y., Wang, G., Li, H."Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway ". Oncology Reports 38, no. 2 (2017): 1233-1239. https://doi.org/10.3892/or.2017.5779
Copy and paste a formatted citation
x
Spandidos Publications style
Tao R, Sun W, Yu D, Qiu W, Yan W, Ding Y, Wang G and Li H: Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway . Oncol Rep 38: 1233-1239, 2017.
APA
Tao, R., Sun, W., Yu, D., Qiu, W., Yan, W., Ding, Y. ... Li, H. (2017). Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway . Oncology Reports, 38, 1233-1239. https://doi.org/10.3892/or.2017.5779
MLA
Tao, R., Sun, W., Yu, D., Qiu, W., Yan, W., Ding, Y., Wang, G., Li, H."Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway ". Oncology Reports 38.2 (2017): 1233-1239.
Chicago
Tao, R., Sun, W., Yu, D., Qiu, W., Yan, W., Ding, Y., Wang, G., Li, H."Sodium cantharidinate induces HepG2 cell apoptosis through LC3 autophagy pathway ". Oncology Reports 38, no. 2 (2017): 1233-1239. https://doi.org/10.3892/or.2017.5779
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