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Article

Role of triosephosphate isomerase and downstream functional genes on gastric cancer

  • Authors:
    • Tingting Chen
    • Zhigang Huang
    • Yunxiao Tian
    • Haiwei Wang
    • Ping Ouyang
    • Haoqin Chen
    • Lili Wu
    • Bode Lin
    • Rongwei He
  • View Affiliations / Copyright

    Affiliations: Department of Epidemiology and Health Statistics, School of Public Health, Guangdong Medical University, Dongguan, Guangdong, P.R. China, Department of Pathology, Handan Central Hospital, Handan, Hebei, P.R. China, Scientific Research Centre, Guangdong Medical University, Dongguan, Guangdong, P.R. China, Department of Internal Medicine, Dalang Hospital of Dongguan City, Dongguan, Guangdong, P.R. China
  • Pages: 1822-1832
    |
    Published online on: July 24, 2017
       https://doi.org/10.3892/or.2017.5846
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Abstract

Triosephosphate isomerase (TPI) is highly expressed in many types of human tumors and is involved in migration and invasion of cancer cells. However, TPI clinicopathological significance and malignant function in gastric cancer (GC) have not been well defined. The present study aimed to examine TPI expression in GC tissue and its biological functions. Furthermore, we investigated its downstream genes by gene chip technology. Our results showed that TPI expression was higher in gastric cancer tissues than adjacent tissues, although no statistical differences were found between TPI expression and clinicopathological factors. TPI overexpression in human gastric carcinoma cell line BGC-823 enhanced cell proliferation, invasion and migration, but did not change cell cycle distribution, while TPI knockdown suppressed proliferation, invasion and migration, induced apoptosis and increased G2/M arrest of human gastric carcinoma cell line MGC-803. Since the cell division cycle associated 5 (CDCA5) was identified as the one with the most decreased expression after TPI knockdown, we investigated its role in MGC-803 cells. The results showed that CDCA5 knockdown also inhibited proliferation, migration, induced apoptosis and increased G2/M arrest similarly to TPI knockdown. CDCA5 overexpression promoted MGC-803 cell proliferation, clone formation and migration abilities. These results indicated that TPI expression level might affect GC cell behavior, suggesting that both TPI and CDCA5 might be considered as potential tumor markers related with GC development and might be potential new targets in GC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Chen T, Huang Z, Tian Y, Wang H, Ouyang P, Chen H, Wu L, Lin B and He R: Role of triosephosphate isomerase and downstream functional genes on gastric cancer. Oncol Rep 38: 1822-1832, 2017.
APA
Chen, T., Huang, Z., Tian, Y., Wang, H., Ouyang, P., Chen, H. ... He, R. (2017). Role of triosephosphate isomerase and downstream functional genes on gastric cancer. Oncology Reports, 38, 1822-1832. https://doi.org/10.3892/or.2017.5846
MLA
Chen, T., Huang, Z., Tian, Y., Wang, H., Ouyang, P., Chen, H., Wu, L., Lin, B., He, R."Role of triosephosphate isomerase and downstream functional genes on gastric cancer". Oncology Reports 38.3 (2017): 1822-1832.
Chicago
Chen, T., Huang, Z., Tian, Y., Wang, H., Ouyang, P., Chen, H., Wu, L., Lin, B., He, R."Role of triosephosphate isomerase and downstream functional genes on gastric cancer". Oncology Reports 38, no. 3 (2017): 1822-1832. https://doi.org/10.3892/or.2017.5846
Copy and paste a formatted citation
x
Spandidos Publications style
Chen T, Huang Z, Tian Y, Wang H, Ouyang P, Chen H, Wu L, Lin B and He R: Role of triosephosphate isomerase and downstream functional genes on gastric cancer. Oncol Rep 38: 1822-1832, 2017.
APA
Chen, T., Huang, Z., Tian, Y., Wang, H., Ouyang, P., Chen, H. ... He, R. (2017). Role of triosephosphate isomerase and downstream functional genes on gastric cancer. Oncology Reports, 38, 1822-1832. https://doi.org/10.3892/or.2017.5846
MLA
Chen, T., Huang, Z., Tian, Y., Wang, H., Ouyang, P., Chen, H., Wu, L., Lin, B., He, R."Role of triosephosphate isomerase and downstream functional genes on gastric cancer". Oncology Reports 38.3 (2017): 1822-1832.
Chicago
Chen, T., Huang, Z., Tian, Y., Wang, H., Ouyang, P., Chen, H., Wu, L., Lin, B., He, R."Role of triosephosphate isomerase and downstream functional genes on gastric cancer". Oncology Reports 38, no. 3 (2017): 1822-1832. https://doi.org/10.3892/or.2017.5846
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