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Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route

  • Authors:
    • Matthieu Meryet-Figuière
    • Charlotte Lecerf
    • Emilie Varin
    • Jean-Luc Coll
    • Marie-Hélène Louis
    • Soizic Dutoit
    • Florence Giffard
    • Cécile Blanc-Fournier
    • Siham Hedir
    • Nicolas Vigneron
    • Emilie Brotin
    • Laurent Pelletier
    • Véronique Josserand
    • Christophe Denoyelle
    • Laurent Poulain
  • View Affiliations / Copyright

    Affiliations: INSERM U1086 ‘ANTICIPE’ Interdisciplinary Research Unit for Cancer Prevention and Treatment, Axe 2: ‘Biology and Innovative Therapeutics for Locally Aggressive Cancers’ (BioTICLA), Comprehensive Cancer Center François Baclesse, 14076 Caen Cedex 5, France, INSERM U1209, Institute of Advanced Biosciences, Institut pour l'Avancée des Biosciences, Centre de Recherche UGA, Site Santé, 38700 La Tronche, France, INSERM U836, Grenoble Institute of Neurosciences, Bâtiment Edmond J. Safra, Chemin Fortuné Ferrini, Site Santé, 38706 La Tronche Cedex, France
    Copyright: © Meryet-Figuière et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1949-1958
    |
    Published online on: August 4, 2017
       https://doi.org/10.3892/or.2017.5882
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Abstract

Ovarian cancer is the leading cause of death from gynecological malignancies worldwide, and innate or acquired chemoresistance of ovarian cancer cells is the major cause of therapeutic failure. It has been demonstrated that the concomitant inhibition of Bcl-xL and Mcl-1 anti-apoptotic activities is able to trigger apoptosis in chemoresistant ovarian cancer cells. In this context, siRNA-mediated Bcl‑xL and Mcl-1 inhibition constitutes an appealing strategy by which to eliminate chemoresistant cancer cells. However, the safest and most efficient way to vectorize siRNAs in vivo is still under debate. In the present study, using in vivo bioluminescence imaging, we evaluated the interest of atelocollagen to vectorize siRNAs by intraperitoneal (i.p.) or intravenous (i.v.) administration in 2 xenografted ovarian cancer models (peritoneal carcinomatosis and subcutaneous tumors in nude mice). Whereas i.p. administration of atelocollagen-vectorized siRNA in the peritoneal carcinomatosis model did not induce any gene downregulation, a 70% transient downregulation of luciferase expression was achieved after i.v. injection of atelocollagen-vectorized siRNA in the subcutaneous (s.c.) model. However, the use of siRNA targeting Bcl-xL or Mcl-1 did not induce target-specific downregulation in vivo in nude mice. Our results therefore show that atelocollagen complex formulation, the administration route, tumor site and the identity of the siRNA target influence the efficiency of atelocollagen‑mediated siRNA delivery.
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Copy and paste a formatted citation
Spandidos Publications style
Meryet-Figuière M, Lecerf C, Varin E, Coll J, Louis M, Dutoit S, Giffard F, Blanc-Fournier C, Hedir S, Vigneron N, Vigneron N, et al: Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route. Oncol Rep 38: 1949-1958, 2017.
APA
Meryet-Figuière, M., Lecerf, C., Varin, E., Coll, J., Louis, M., Dutoit, S. ... Poulain, L. (2017). Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route. Oncology Reports, 38, 1949-1958. https://doi.org/10.3892/or.2017.5882
MLA
Meryet-Figuière, M., Lecerf, C., Varin, E., Coll, J., Louis, M., Dutoit, S., Giffard, F., Blanc-Fournier, C., Hedir, S., Vigneron, N., Brotin, E., Pelletier, L., Josserand, V., Denoyelle, C., Poulain, L."Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route". Oncology Reports 38.4 (2017): 1949-1958.
Chicago
Meryet-Figuière, M., Lecerf, C., Varin, E., Coll, J., Louis, M., Dutoit, S., Giffard, F., Blanc-Fournier, C., Hedir, S., Vigneron, N., Brotin, E., Pelletier, L., Josserand, V., Denoyelle, C., Poulain, L."Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route". Oncology Reports 38, no. 4 (2017): 1949-1958. https://doi.org/10.3892/or.2017.5882
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Spandidos Publications style
Meryet-Figuière M, Lecerf C, Varin E, Coll J, Louis M, Dutoit S, Giffard F, Blanc-Fournier C, Hedir S, Vigneron N, Vigneron N, et al: Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route. Oncol Rep 38: 1949-1958, 2017.
APA
Meryet-Figuière, M., Lecerf, C., Varin, E., Coll, J., Louis, M., Dutoit, S. ... Poulain, L. (2017). Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route. Oncology Reports, 38, 1949-1958. https://doi.org/10.3892/or.2017.5882
MLA
Meryet-Figuière, M., Lecerf, C., Varin, E., Coll, J., Louis, M., Dutoit, S., Giffard, F., Blanc-Fournier, C., Hedir, S., Vigneron, N., Brotin, E., Pelletier, L., Josserand, V., Denoyelle, C., Poulain, L."Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route". Oncology Reports 38.4 (2017): 1949-1958.
Chicago
Meryet-Figuière, M., Lecerf, C., Varin, E., Coll, J., Louis, M., Dutoit, S., Giffard, F., Blanc-Fournier, C., Hedir, S., Vigneron, N., Brotin, E., Pelletier, L., Josserand, V., Denoyelle, C., Poulain, L."Atelocollagen-mediated in vivo siRNA transfection in ovarian carcinoma is influenced by tumor site, siRNA target and administration route". Oncology Reports 38, no. 4 (2017): 1949-1958. https://doi.org/10.3892/or.2017.5882
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