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Article

miR106b regulates retinoblastoma Y79 cells through Runx3

  • Authors:
    • Ge Yang
    • Yang Fu
    • Luxi Zhang
    • Xiaoyan Lu
    • Qiuming Li
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 3039-3043
    |
    Published online on: August 30, 2017
       https://doi.org/10.3892/or.2017.5931
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Abstract

MicroRNAs are increasingly recognized as important regulators of cancer. The aim of the present study was to investigate the role of miR-106b in the regulation of Y79 retinoblastoma. Y79 cells were transfected with antisense oligonucleotides (ASO) against miR-106b (ASO-miR-106b) or ASO-control. After transfection, the levels of miR-106b were monitored with real-time PCR (RT-PCR). The effects of ASO-miR-106b transfection on cell viability was evaluated by Cell Counting Kit-8 (CCK-8) analysis at 24, 48 and 72 h after transfection. Subsequently, the cells were stained with Annexin V-FITC and propidium iodide (PI) and subjected to flow cytometry to assess cell apoptosis. Transwell assay was used to analyze cell migration. Changes in Runt-related transcription factor 3 (Runx3) mRNA and proteins levels were also evaluated. miR-106b was downregulated by ASO-miR-106b at 48 and 72 h after transfection, accompanied by a decrease in cell viability and proliferation, as well as an increase in cell apoptosis. Transwell analysis indicated that cells treated with ASO-miR-106b exhibited significantly lower cell migratory abilities. The mRNA and protein level of Runx3 were upregulated after transfection. These results demonstrated that suppression of miR-106b inhibited Y79 cell proliferation and migration. The upregulation of Runx3 after miR-106b suppression ascertained that Runx3 is a tumor-suppressor in retinoblastoma and is a target of miR-106b.
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1 

Dimaras H, Kimani K, Dimba EA, Gronsdahl P, White A, Chan HS and Gallie BL: Retinoblastoma. Lancet. 379:1436–1446. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Liang Y and Cao R: Employment assistance policies of Chinese government play positive roles! The impact of post-earthquake employment assistance policies on the health-related quality of life of Chinese earthquake populations. Soc Indic Res. 120:835–857. 2014. View Article : Google Scholar

3 

Liang Y and Guo M: Utilization of health services and health-related quality of life research of rural-to-urban migrants in china: A cross-sectional analysis. Soc Indic Res. 120:277–295. 2015. View Article : Google Scholar

4 

Clarke AR, Maandag ER, van Roon M, van der Lugt NM, van der Valk M, Hooper ML, Berns A and te Riele H: Requirement for a functional Rb-1 gene in murine development. Nature. 359:328–330. 1992. View Article : Google Scholar : PubMed/NCBI

5 

Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Conkrite K, Sundby M, Mukai S, Thomson JM, Mu D, Hammond SM and MacPherson D: miR-17~92 cooperates with RB pathway mutations to promote retinoblastoma. Genes Dev. 25:1734–1745. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Xu Y, Wang K, Gao W, Zhang C, Huang F, Wen S and Wang B: MicroRNA-106b regulates the tumor suppressor RUNX3 in laryngeal carcinoma cells. FEBS Lett. 587:3166–3174. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Trompeter HI, Abbad H, Iwaniuk KM, Hafner M, Renwick N, Tuschl T, Schira J, Müller HW and Wernet P: MicroRNAs MiR-17, MiR-20a, and MiR-106b act in concert to modulate E2F activity on cell cycle arrest during neuronal lineage differentiation of USSC. PLoS One. 6:e161382011. View Article : Google Scholar : PubMed/NCBI

9 

Tucker A and Sharpe P: The cutting-edge of mammalian development; how the embryo makes teeth. Nat Rev Genet. 5:499–508. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Kim EJ, Kim YJ, Jeong P, Ha YS, Bae SC and Kim WJ: Methylation of the RUNX3 promoter as a potential prognostic marker for bladder tumor. J Urol. 180:1141–1145. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Guo WH, Weng LQ, Ito K, Chen LF, Nakanishi H, Tatematsu M and Ito Y: Inhibition of growth of mouse gastric cancer cells by Runx3, a novel tumor suppressor. Oncogene. 21:8351–8355. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Mueller W, Nutt CL, Ehrich M, Riemenschneider MJ, von Deimling A, van den Boom D and Louis DN: Downregulation of RUNX3 and TES by hypermethylation in glioblastoma. Oncogene. 26:583–593. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Honavar SG, Shields CL, Shields JA, Demirci H and Naduvilath TJ: Intraocular surgery after treatment of retinoblastoma. Arch Ophthalmol. 119:1613–1621. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Errico A: Cancer therapy: Retinoblastoma - chemotherapy increases the risk of secondary cancer. Nat Rev Clin Oncol. 11:623. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Smith SJ and Smith BD: Evaluating the risk of extraocular tumour spread following intravitreal injection therapy for retinoblastoma: A systematic review. Br J Ophthalmol. 97:1231–1236. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Tong AW and Nemunaitis J: Modulation of miRNA activity in human cancer: A new paradigm for cancer gene therapy? Cancer Gene Ther. 15:341–355. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Liang Y: Satisfaction with economic and social rights and quality of life in a post-disaster zone in China: Evidence from earthquake-prone Sichuan. Disaster Med Public Health Prep. 9:111–118. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Liang Y: Correlations between health-related quality of life and interpersonal trust: Comparisons between two generations of Chinese rural-to-urban migrants. Soc Indic Res. 123:677–700. 2015. View Article : Google Scholar

19 

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:pp. 2257–2261. 2006; View Article : Google Scholar : PubMed/NCBI

20 

Feng S, Cong S, Zhang X, Bao X, Wang W, Li H, Wang Z, Wang G, Xu J, Du B, et al: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells. Nucleic Acids Res. 39:6669–6678. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Zhao JJ, Yang J, Lin J, Yao N, Zhu Y, Zheng J, Xu J, Cheng JQ, Lin JY and Ma X: Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis. Childs Nerv Syst. 25:13–20. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Zhang J, Benavente CA, McEvoy J, Flores-Otero J, Ding L, Chen X, Ulyanov A, Wu G, Wilson M, Wang J, et al: A novel retinoblastoma therapy from genomic and epigenetic analyses. Nature. 481:329–334. 2012.PubMed/NCBI

23 

Zheng L, Zhang Y, Liu Y, Zhou M, Lu Y, Yuan L, Zhang C, Hong M, Wang S and Li X: MiR-106b induces cell radioresistance via the PTEN/PI3K/AKT pathways and p21 in colorectal cancer. J Transl Med. 13:2522015. View Article : Google Scholar : PubMed/NCBI

24 

Gong C, Qu S, Liu B, Pan S, Jiao Y, Nie Y, Su F, Liu Q and Song E: MiR-106b expression determines the proliferation paradox of TGF-β in breast cancer cells. Oncogene. 34:84–93. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Wei D, Gong W, Oh SC, Li Q, Kim WD, Wang L, Le X, Yao J, Wu TT, Huang S, et al: Loss of RUNX3 expression significantly affects the clinical outcome of gastric cancer patients and its restoration causes drastic suppression of tumor growth and metastasis. Cancer Res. 65:4809–4816. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Smith AL, Iwanaga R, Drasin DJ, Micalizzi DS, Vartuli RL and Ford HL: Abstract A20: The Six1-regulated miR-106b-25 cluster is a mediator of the tumor promotional effects of TGF-β signaling in human breast cancer. Cancer Res. 72 Suppl 2:A20. 2012. View Article : Google Scholar

27 

Li YL, Wang YH, Zhou H, Yong YH and Cao YD: miR-106b promoted growth and inhibited apoptosis of nasopharyngeal carcinoma cells by suppressing the tumor suppressor PTEN. Int J Clin Exp Pathol. 9:7078–7086. 2016.

28 

Malcomson FC, Willis ND, McCallum I, Xie L, Kelly S, Bradburn M, Belshaw NJ, Johnson IT and Mathers JC: Differences in the expression of microRNAs implicated in colorectal carcinogenesis and involved in the WNT signalling pathway in the macroscopically-normal epithelium of people at higher-risk of colorectal cancer. Proc Nutr Soc. 74(OCE1): pp. E462015; View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yang G, Fu Y, Zhang L, Lu X and Li Q: miR106b regulates retinoblastoma Y79 cells through Runx3. Oncol Rep 38: 3039-3043, 2017.
APA
Yang, G., Fu, Y., Zhang, L., Lu, X., & Li, Q. (2017). miR106b regulates retinoblastoma Y79 cells through Runx3. Oncology Reports, 38, 3039-3043. https://doi.org/10.3892/or.2017.5931
MLA
Yang, G., Fu, Y., Zhang, L., Lu, X., Li, Q."miR106b regulates retinoblastoma Y79 cells through Runx3". Oncology Reports 38.5 (2017): 3039-3043.
Chicago
Yang, G., Fu, Y., Zhang, L., Lu, X., Li, Q."miR106b regulates retinoblastoma Y79 cells through Runx3". Oncology Reports 38, no. 5 (2017): 3039-3043. https://doi.org/10.3892/or.2017.5931
Copy and paste a formatted citation
x
Spandidos Publications style
Yang G, Fu Y, Zhang L, Lu X and Li Q: miR106b regulates retinoblastoma Y79 cells through Runx3. Oncol Rep 38: 3039-3043, 2017.
APA
Yang, G., Fu, Y., Zhang, L., Lu, X., & Li, Q. (2017). miR106b regulates retinoblastoma Y79 cells through Runx3. Oncology Reports, 38, 3039-3043. https://doi.org/10.3892/or.2017.5931
MLA
Yang, G., Fu, Y., Zhang, L., Lu, X., Li, Q."miR106b regulates retinoblastoma Y79 cells through Runx3". Oncology Reports 38.5 (2017): 3039-3043.
Chicago
Yang, G., Fu, Y., Zhang, L., Lu, X., Li, Q."miR106b regulates retinoblastoma Y79 cells through Runx3". Oncology Reports 38, no. 5 (2017): 3039-3043. https://doi.org/10.3892/or.2017.5931
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