miR106b regulates retinoblastoma Y79 cells through Runx3

  • Authors:
    • Ge Yang
    • Yang Fu
    • Luxi Zhang
    • Xiaoyan Lu
    • Qiuming Li
  • View Affiliations

  • Published online on: August 30, 2017     https://doi.org/10.3892/or.2017.5931
  • Pages: 3039-3043
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Abstract

MicroRNAs are increasingly recognized as important regulators of cancer. The aim of the present study was to investigate the role of miR-106b in the regulation of Y79 retinoblastoma. Y79 cells were transfected with antisense oligonucleotides (ASO) against miR-106b (ASO-miR-106b) or ASO-control. After transfection, the levels of miR-106b were monitored with real-time PCR (RT-PCR). The effects of ASO-miR-106b transfection on cell viability was evaluated by Cell Counting Kit-8 (CCK-8) analysis at 24, 48 and 72 h after transfection. Subsequently, the cells were stained with Annexin V-FITC and propidium iodide (PI) and subjected to flow cytometry to assess cell apoptosis. Transwell assay was used to analyze cell migration. Changes in Runt-related transcription factor 3 (Runx3) mRNA and proteins levels were also evaluated. miR-106b was downregulated by ASO-miR-106b at 48 and 72 h after transfection, accompanied by a decrease in cell viability and proliferation, as well as an increase in cell apoptosis. Transwell analysis indicated that cells treated with ASO-miR-106b exhibited significantly lower cell migratory abilities. The mRNA and protein level of Runx3 were upregulated after transfection. These results demonstrated that suppression of miR-106b inhibited Y79 cell proliferation and migration. The upregulation of Runx3 after miR-106b suppression ascertained that Runx3 is a tumor-suppressor in retinoblastoma and is a target of miR-106b.
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November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang G, Fu Y, Zhang L, Lu X and Li Q: miR106b regulates retinoblastoma Y79 cells through Runx3. Oncol Rep 38: 3039-3043, 2017
APA
Yang, G., Fu, Y., Zhang, L., Lu, X., & Li, Q. (2017). miR106b regulates retinoblastoma Y79 cells through Runx3. Oncology Reports, 38, 3039-3043. https://doi.org/10.3892/or.2017.5931
MLA
Yang, G., Fu, Y., Zhang, L., Lu, X., Li, Q."miR106b regulates retinoblastoma Y79 cells through Runx3". Oncology Reports 38.5 (2017): 3039-3043.
Chicago
Yang, G., Fu, Y., Zhang, L., Lu, X., Li, Q."miR106b regulates retinoblastoma Y79 cells through Runx3". Oncology Reports 38, no. 5 (2017): 3039-3043. https://doi.org/10.3892/or.2017.5931