Naringenin inhibits alcoholic injury by improving lipid metabolism and reducing apoptosis in zebrafish larvae

Lin,Haiyan; Zhou,Zhenting; Zhong,Weichao; Huang,Peng; Ma,Ning; Zhang,Yuqing; Zhou,Chuying; Lai,Yuling; Huang,Shaohui; An,Haiyan; Sun,Xuegang; Gao,Lei; Lv,Zhiping

doi:10.3892/or.2017.5965

Naringenin inhibits alcoholic injury by improving lipid metabolism and reducing apoptosis in zebrafish larvae

Authors:
- Haiyan Lin
- Zhenting Zhou
- Weichao Zhong
- Peng Huang
- Ning Ma
- Yuqing Zhang
- Chuying Zhou
- Yuling Lai
- Shaohui Huang
- Haiyan An
- Xuegang Sun
- Lei Gao
- Zhiping Lv
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Affiliations: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Liver Diseases, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, Guangdong 518033, P.R. China, Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, Institute of Genetic Engineering, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Published online on: September 19, 2017 https://doi.org/10.3892/or.2017.5965
Pages: 2877-2884

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Abstract

Alcoholic liver disease (ALD) includes a spectrum of hepatic abnormalities that range from isolated alcoholic steatosis to steatohepatitis and cirrhosis. Naringenin, a predominant flavanone in grapefruit, increases resistance to oxidative stress and inflammation and protects against multiple organ injury in various animal models. However, the specific mechanisms responsible for protection against alcoholic injury are poorly understood. In the present study, we aimed to investigate the effect of naringenin on alcoholic events and the molecular regulatory mechanisms of naringenin in the liver and whole body of zebrafish larvae following exposure to 350 mmol/l ethanol for 32 h. Zebrafish larvae {4 days post‑fertilization (dpf); wild-type (WT) and a transgenic line with liver-specific eGFP expression [Tg(lfabp10α-eGFP)]} were used to establish an alcoholic fatty liver model in order to evaluate the effects of naringenin treatment on anti-alcoholic injury. Naringenin significantly reduced alcoholic liver morphological phenotypes and the expression of alcohol and lipid metabolism-related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, fabp10α, fads2 and echs1, in zebrafish larvae. Naringenin also attenuated hepatic apoptosis in larvae as detected by TUNEL staining, consistent with the expression of critical biomarkers of endoplasmic reticulum stress and of DNA damage genes (chop, gadd45αa and edem1). The present study showed that naringenin inhibited alcohol-induced liver steatosis and injury in zebrafish larvae by reducing apoptosis and DNA damage and by harmonizing alcohol and lipid metabolism.

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