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Article

Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells

  • Authors:
    • Yoon Jae Kim
    • Seok Hoo Jeong
    • Eun-Kyung Kim
    • Eui Joo Kim
    • Jae Hee Cho
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology, Department of Internal Medicine, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea, Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University, International St. Mary's Hospital, Incheon 22711, Republic of Korea
  • Pages: 3632-3638
    |
    Published online on: October 19, 2017
       https://doi.org/10.3892/or.2017.6045
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Abstract

Reactive oxygen species (ROS) play a key role in cancer development and progression. Ursodeoxycholic acid (UDCA) may possess antioxidant, anti-inflammatory and chemoprophylatic effects. Therefore, we aimed to investigate the effects and mechanisms of UDCA treatment on pancreatic cancer cells. The pancreatic cancer cell lines HPAC and Capan-1 were treated with 0.2 mM UDCA. To examine alterations in the levels of intracellular ROS, the DCF-DA stain was used and both stemness and epithelial-mesenchymal transition (EMT)-related genes were quantified using qRT-PCR and western blot analysis. The pancreatic cancer sphere culture was performed following seven days of treatment with 0.2 mM UDCA, as an indicator of stemness. Following treatment with UDCA, the level of intracellular ROS was decreased in the pancreatic cancer cells. UDCA decreased both the phosphorylation of STAT3 and the expression of peroxiredoxin II (Prx2). Furthermore, the treatment resulted in the upregulation of E-cadherin and in the downregulation of N-cadherin. In addition, UDCA decreased the expression of sex determining region Y-box 2 (Sox2) and it diminished the number of pancreatic cancer spheres formed. In conclusion UDCA suppressed the levels of intracellular ROS and Prx2 and it decreased EMT and stem cell formation in pancreatic cancer cells. Therefore, UDCA may provide favorable therapeutic benefits, through its antioxidant effects, for patients with pancreatic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kim YJ, Jeong SH, Kim E, Kim EJ and Cho JH: Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells. Oncol Rep 38: 3632-3638, 2017.
APA
Kim, Y.J., Jeong, S.H., Kim, E., Kim, E.J., & Cho, J.H. (2017). Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells. Oncology Reports, 38, 3632-3638. https://doi.org/10.3892/or.2017.6045
MLA
Kim, Y. J., Jeong, S. H., Kim, E., Kim, E. J., Cho, J. H."Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells". Oncology Reports 38.6 (2017): 3632-3638.
Chicago
Kim, Y. J., Jeong, S. H., Kim, E., Kim, E. J., Cho, J. H."Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells". Oncology Reports 38, no. 6 (2017): 3632-3638. https://doi.org/10.3892/or.2017.6045
Copy and paste a formatted citation
x
Spandidos Publications style
Kim YJ, Jeong SH, Kim E, Kim EJ and Cho JH: Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells. Oncol Rep 38: 3632-3638, 2017.
APA
Kim, Y.J., Jeong, S.H., Kim, E., Kim, E.J., & Cho, J.H. (2017). Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells. Oncology Reports, 38, 3632-3638. https://doi.org/10.3892/or.2017.6045
MLA
Kim, Y. J., Jeong, S. H., Kim, E., Kim, E. J., Cho, J. H."Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells". Oncology Reports 38.6 (2017): 3632-3638.
Chicago
Kim, Y. J., Jeong, S. H., Kim, E., Kim, E. J., Cho, J. H."Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells". Oncology Reports 38, no. 6 (2017): 3632-3638. https://doi.org/10.3892/or.2017.6045
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